Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2144 and Pf1N1B4_2145 overlap by 29 nucleotides Pf1N1B4_2145 and Pf1N1B4_2146 are separated by 97 nucleotides
Pf1N1B4_2144 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 2,454,539 to 2,455,843
_2144
Pf1N1B4_2145 - Translation initiation inhibitor, at 2,455,815 to 2,456,168
_2145
Pf1N1B4_2146 - Alanine racemase (EC 5.1.1.1), at 2,456,266 to 2,457,339
_2146
Position (kb)
2455
2456
2457 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2454.878 kb on + strand, within Pf1N1B4_2144 at 2454.927 kb on - strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.349 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.774 kb on + strand at 2455.774 kb on + strand at 2455.874 kb on + strand, within Pf1N1B4_2145 at 2456.013 kb on + strand, within Pf1N1B4_2145 at 2456.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,454,878 + Pf1N1B4_2144 0.26 +0.4 2,454,927 - Pf1N1B4_2144 0.30 +1.1 2,455,283 + Pf1N1B4_2144 0.57 +0.8 2,455,283 + Pf1N1B4_2144 0.57 +1.3 2,455,305 - Pf1N1B4_2144 0.59 +0.6 2,455,305 - Pf1N1B4_2144 0.59 +0.1 2,455,349 + Pf1N1B4_2144 0.62 -1.4 2,455,430 + Pf1N1B4_2144 0.68 -0.9 2,455,430 + Pf1N1B4_2144 0.68 -0.2 2,455,430 + Pf1N1B4_2144 0.68 +0.5 2,455,430 + Pf1N1B4_2144 0.68 +2.0 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 +1.0 2,455,430 + Pf1N1B4_2144 0.68 +2.0 2,455,430 + Pf1N1B4_2144 0.68 -0.7 2,455,430 + Pf1N1B4_2144 0.68 +1.4 2,455,430 + Pf1N1B4_2144 0.68 +0.7 2,455,430 + Pf1N1B4_2144 0.68 +1.5 2,455,430 + Pf1N1B4_2144 0.68 +2.8 2,455,430 + Pf1N1B4_2144 0.68 +1.4 2,455,430 + Pf1N1B4_2144 0.68 +0.9 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 +1.2 2,455,430 + Pf1N1B4_2144 0.68 +1.7 2,455,430 + Pf1N1B4_2144 0.68 +0.8 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 +1.4 2,455,430 + Pf1N1B4_2144 0.68 +2.0 2,455,430 + Pf1N1B4_2144 0.68 +1.5 2,455,430 + Pf1N1B4_2144 0.68 +1.1 2,455,430 + Pf1N1B4_2144 0.68 +0.8 2,455,431 - Pf1N1B4_2144 0.68 -0.2 2,455,431 - Pf1N1B4_2144 0.68 +0.9 2,455,431 - Pf1N1B4_2144 0.68 +2.7 2,455,431 - Pf1N1B4_2144 0.68 +0.1 2,455,431 - Pf1N1B4_2144 0.68 +1.8 2,455,431 - Pf1N1B4_2144 0.68 +0.7 2,455,432 + Pf1N1B4_2144 0.68 +0.2 2,455,432 + Pf1N1B4_2144 0.68 +0.1 2,455,432 + Pf1N1B4_2144 0.68 -0.5 2,455,432 + Pf1N1B4_2144 0.68 +2.3 2,455,432 + Pf1N1B4_2144 0.68 -0.5 2,455,433 - Pf1N1B4_2144 0.69 -0.2 2,455,433 - Pf1N1B4_2144 0.69 -0.6 2,455,433 - Pf1N1B4_2144 0.69 +1.9 2,455,774 + +0.6 2,455,774 + +1.5 2,455,874 + Pf1N1B4_2145 0.17 -0.4 2,456,013 + Pf1N1B4_2145 0.56 -1.2 2,456,251 + +0.1
Or see this region's nucleotide sequence