Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_188

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_187 and Pf1N1B4_188 are separated by 168 nucleotidesPf1N1B4_188 and Pf1N1B4_189 are separated by 98 nucleotidesPf1N1B4_189 and Pf1N1B4_190 are separated by 36 nucleotides Pf1N1B4_187 - Threonine and homoserine efflux system, at 236,464 to 237,351 _187 Pf1N1B4_188 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 237,520 to 237,801 _188 Pf1N1B4_189 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 237,900 to 238,640 _189 Pf1N1B4_190 - 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent, at 238,677 to 239,273 _190 Position (kb) 237 238Strain fitness (log2 ratio) -2 -1 0 1 2 3at 236.640 kb on - strand, within Pf1N1B4_187at 236.731 kb on + strand, within Pf1N1B4_187at 236.731 kb on + strandat 236.731 kb on + strand, within Pf1N1B4_187at 236.731 kb on + strand, within Pf1N1B4_187at 236.731 kb on + strandat 236.731 kb on + strand, within Pf1N1B4_187at 236.731 kb on + strand, within Pf1N1B4_187at 236.731 kb on + strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.732 kb on - strand, within Pf1N1B4_187at 236.733 kb on + strand, within Pf1N1B4_187at 236.733 kb on + strand, within Pf1N1B4_187at 236.733 kb on + strand, within Pf1N1B4_187at 236.733 kb on + strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strandat 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.734 kb on - strand, within Pf1N1B4_187at 236.803 kb on - strand, within Pf1N1B4_187at 236.806 kb on + strand, within Pf1N1B4_187at 236.806 kb on + strand, within Pf1N1B4_187at 236.862 kb on - strand, within Pf1N1B4_187at 236.867 kb on + strand, within Pf1N1B4_187at 236.868 kb on - strand, within Pf1N1B4_187at 236.953 kb on + strand, within Pf1N1B4_187at 236.953 kb on + strand, within Pf1N1B4_187at 236.953 kb on + strand, within Pf1N1B4_187at 236.953 kb on + strand, within Pf1N1B4_187at 236.953 kb on + strand, within Pf1N1B4_187at 236.954 kb on - strand, within Pf1N1B4_187at 236.954 kb on - strand, within Pf1N1B4_187at 236.954 kb on - strand, within Pf1N1B4_187at 237.035 kb on + strand, within Pf1N1B4_187at 237.036 kb on - strand, within Pf1N1B4_187at 237.133 kb on + strand, within Pf1N1B4_187at 237.165 kb on - strand, within Pf1N1B4_187at 237.244 kb on + strand, within Pf1N1B4_187at 237.244 kb on + strand, within Pf1N1B4_187at 237.244 kb on + strand, within Pf1N1B4_187at 237.245 kb on - strand, within Pf1N1B4_187at 237.321 kb on + strandat 237.321 kb on + strandat 237.374 kb on + strandat 237.389 kb on + strandat 237.390 kb on - strandat 237.390 kb on - strandat 237.409 kb on + strandat 237.409 kb on + strandat 237.409 kb on + strandat 237.409 kb on + strandat 237.616 kb on - strand, within Pf1N1B4_188at 237.655 kb on + strand, within Pf1N1B4_188at 237.691 kb on + strand, within Pf1N1B4_188at 237.691 kb on + strand, within Pf1N1B4_188at 237.800 kb on - strandat 237.996 kb on + strand, within Pf1N1B4_189at 237.997 kb on - strand, within Pf1N1B4_189at 237.997 kb on - strand, within Pf1N1B4_189at 237.999 kb on - strand, within Pf1N1B4_189at 238.225 kb on - strand, within Pf1N1B4_189at 238.563 kb on + strandat 238.563 kb on + strand, within Pf1N1B4_189at 238.563 kb on + strandat 238.563 kb on + strand, within Pf1N1B4_189at 238.563 kb on + strand, within Pf1N1B4_189at 238.564 kb on - strand, within Pf1N1B4_189at 238.564 kb on - strand, within Pf1N1B4_189at 238.564 kb on - strand, within Pf1N1B4_189at 238.564 kb on - strand, within Pf1N1B4_189at 238.569 kb on + strandat 238.569 kb on + strandat 238.570 kb on - strandat 238.570 kb on - strandat 238.570 kb on - strandat 238.658 kb on + strandat 238.658 kb on + strandat 238.659 kb on - strandat 238.659 kb on - strandat 238.725 kb on + strandat 238.734 kb on + strandat 238.734 kb on + strandat 238.734 kb on + strandat 238.736 kb on + strandat 238.737 kb on - strand, within Pf1N1B4_190at 238.782 kb on + strand, within Pf1N1B4_190at 238.782 kb on + strand, within Pf1N1B4_190at 238.783 kb on - strand, within Pf1N1B4_190at 238.783 kb on - strand, within Pf1N1B4_190at 238.783 kb on - strand, within Pf1N1B4_190

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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236,640 - Pf1N1B4_187 0.20 -0.2
236,731 + Pf1N1B4_187 0.30 -1.8
236,731 + +3.3
236,731 + Pf1N1B4_187 0.30 +1.0
236,731 + Pf1N1B4_187 0.30 +1.2
236,731 + -0.2
236,731 + Pf1N1B4_187 0.30 -0.2
236,731 + Pf1N1B4_187 0.30 +0.2
236,731 + Pf1N1B4_187 0.30 +0.4
236,732 - Pf1N1B4_187 0.30 -1.6
236,732 - Pf1N1B4_187 0.30 -0.2
236,732 - Pf1N1B4_187 0.30 +0.8
236,732 - Pf1N1B4_187 0.30 +1.3
236,732 - Pf1N1B4_187 0.30 -1.2
236,732 - Pf1N1B4_187 0.30 -0.6
236,732 - Pf1N1B4_187 0.30 -0.1
236,732 - Pf1N1B4_187 0.30 -1.6
236,732 - Pf1N1B4_187 0.30 +0.9
236,733 + Pf1N1B4_187 0.30 -0.2
236,733 + Pf1N1B4_187 0.30 +0.3
236,733 + Pf1N1B4_187 0.30 +1.2
236,733 + Pf1N1B4_187 0.30 +0.4
236,734 - Pf1N1B4_187 0.30 +0.6
236,734 - Pf1N1B4_187 0.30 -0.1
236,734 - -0.2
236,734 - Pf1N1B4_187 0.30 +0.2
236,734 - Pf1N1B4_187 0.30 +0.0
236,734 - Pf1N1B4_187 0.30 -1.2
236,734 - Pf1N1B4_187 0.30 +1.4
236,734 - Pf1N1B4_187 0.30 -0.4
236,734 - Pf1N1B4_187 0.30 -1.4
236,803 - Pf1N1B4_187 0.38 +0.0
236,806 + Pf1N1B4_187 0.39 +0.1
236,806 + Pf1N1B4_187 0.39 +0.4
236,862 - Pf1N1B4_187 0.45 +2.1
236,867 + Pf1N1B4_187 0.45 -0.2
236,868 - Pf1N1B4_187 0.45 +1.3
236,953 + Pf1N1B4_187 0.55 -1.8
236,953 + Pf1N1B4_187 0.55 -0.2
236,953 + Pf1N1B4_187 0.55 -0.2
236,953 + Pf1N1B4_187 0.55 +3.1
236,953 + Pf1N1B4_187 0.55 -0.2
236,954 - Pf1N1B4_187 0.55 +2.6
236,954 - Pf1N1B4_187 0.55 +0.3
236,954 - Pf1N1B4_187 0.55 +1.9
237,035 + Pf1N1B4_187 0.64 -0.6
237,036 - Pf1N1B4_187 0.64 +0.8
237,133 + Pf1N1B4_187 0.75 +0.4
237,165 - Pf1N1B4_187 0.79 +0.8
237,244 + Pf1N1B4_187 0.88 -0.8
237,244 + Pf1N1B4_187 0.88 -0.6
237,244 + Pf1N1B4_187 0.88 +0.3
237,245 - Pf1N1B4_187 0.88 -0.2
237,321 + -0.2
237,321 + -0.1
237,374 + +0.1
237,389 + +0.1
237,390 - +0.4
237,390 - -1.7
237,409 + -0.1
237,409 + -0.8
237,409 + +0.3
237,409 + +0.6
237,616 - Pf1N1B4_188 0.34 -2.0
237,655 + Pf1N1B4_188 0.48 +0.4
237,691 + Pf1N1B4_188 0.61 -0.5
237,691 + Pf1N1B4_188 0.61 +0.2
237,800 - +0.8
237,996 + Pf1N1B4_189 0.13 +0.8
237,997 - Pf1N1B4_189 0.13 -0.2
237,997 - Pf1N1B4_189 0.13 -0.1
237,999 - Pf1N1B4_189 0.13 +0.0
238,225 - Pf1N1B4_189 0.44 -0.2
238,563 + -0.8
238,563 + Pf1N1B4_189 0.89 -0.4
238,563 + -0.2
238,563 + Pf1N1B4_189 0.89 +1.8
238,563 + Pf1N1B4_189 0.89 -0.6
238,564 - Pf1N1B4_189 0.90 +0.8
238,564 - Pf1N1B4_189 0.90 +0.7
238,564 - Pf1N1B4_189 0.90 -0.4
238,564 - Pf1N1B4_189 0.90 +2.8
238,569 + -0.7
238,569 + +2.8
238,570 - +0.4
238,570 - -0.8
238,570 - -0.6
238,658 + +0.5
238,658 + +0.4
238,659 - +1.4
238,659 - +0.9
238,725 + +1.1
238,734 + +0.5
238,734 + -0.4
238,734 + +2.4
238,736 + -1.2
238,737 - Pf1N1B4_190 0.10 +0.8
238,782 + Pf1N1B4_190 0.18 +0.1
238,782 + Pf1N1B4_190 0.18 -0.6
238,783 - Pf1N1B4_190 0.18 +0.8
238,783 - Pf1N1B4_190 0.18 +0.8
238,783 - Pf1N1B4_190 0.18 +1.4

Or see this region's nucleotide sequence