Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1502 and Pf1N1B4_1503 overlap by 20 nucleotides Pf1N1B4_1503 and Pf1N1B4_1504 are separated by 9 nucleotides Pf1N1B4_1504 and Pf1N1B4_1505 overlap by 4 nucleotides Pf1N1B4_1505 and Pf1N1B4_1506 are separated by 373 nucleotides
Pf1N1B4_1502 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,736,019 to 1,736,828
_1502
Pf1N1B4_1503 - Acyl carrier protein (ACP1), at 1,736,809 to 1,737,069
_1503
Pf1N1B4_1504 - Acyl carrier protein, at 1,737,079 to 1,737,333
_1504
Pf1N1B4_1505 - FIG017861: hypothetical protein, at 1,737,330 to 1,737,875
_1505
Pf1N1B4_1506 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 1,738,249 to 1,739,550
_1506
Position (kb)
1737
1738 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1736.122 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.462 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.772 kb on + strand at 1736.772 kb on + strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.789 kb on + strand at 1736.789 kb on + strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.793 kb on - strand at 1736.972 kb on - strand, within Pf1N1B4_1503 at 1737.010 kb on - strand, within Pf1N1B4_1503 at 1737.175 kb on + strand, within Pf1N1B4_1504 at 1737.176 kb on - strand, within Pf1N1B4_1504 at 1737.176 kb on - strand, within Pf1N1B4_1504 at 1737.280 kb on - strand, within Pf1N1B4_1504 at 1737.375 kb on + strand at 1737.376 kb on - strand at 1737.376 kb on - strand at 1737.376 kb on - strand at 1737.390 kb on + strand, within Pf1N1B4_1505 at 1737.390 kb on + strand, within Pf1N1B4_1505 at 1737.391 kb on - strand, within Pf1N1B4_1505 at 1737.391 kb on - strand, within Pf1N1B4_1505 at 1737.618 kb on + strand, within Pf1N1B4_1505 at 1737.618 kb on + strand, within Pf1N1B4_1505 at 1737.619 kb on - strand, within Pf1N1B4_1505 at 1737.668 kb on - strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.807 kb on + strand, within Pf1N1B4_1505 at 1737.807 kb on + strand, within Pf1N1B4_1505 at 1737.808 kb on - strand, within Pf1N1B4_1505 at 1737.860 kb on - strand at 1738.089 kb on + strand at 1738.089 kb on + strand at 1738.089 kb on + strand at 1738.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,736,122 + Pf1N1B4_1502 0.13 +2.5 1,736,262 + Pf1N1B4_1502 0.30 +0.7 1,736,262 + Pf1N1B4_1502 0.30 +0.6 1,736,262 + Pf1N1B4_1502 0.30 +0.6 1,736,263 - Pf1N1B4_1502 0.30 -0.3 1,736,263 - Pf1N1B4_1502 0.30 -0.6 1,736,263 - Pf1N1B4_1502 0.30 +1.3 1,736,263 - Pf1N1B4_1502 0.30 -0.7 1,736,263 - Pf1N1B4_1502 0.30 -1.5 1,736,263 - Pf1N1B4_1502 0.30 -1.0 1,736,437 - Pf1N1B4_1502 0.52 +0.1 1,736,437 - Pf1N1B4_1502 0.52 -2.1 1,736,437 - Pf1N1B4_1502 0.52 -0.6 1,736,437 - Pf1N1B4_1502 0.52 -0.1 1,736,437 - Pf1N1B4_1502 0.52 -1.3 1,736,462 + Pf1N1B4_1502 0.55 +1.0 1,736,710 + Pf1N1B4_1502 0.85 +1.3 1,736,710 + Pf1N1B4_1502 0.85 +0.7 1,736,710 + Pf1N1B4_1502 0.85 +0.7 1,736,711 - Pf1N1B4_1502 0.85 -1.9 1,736,711 - Pf1N1B4_1502 0.85 -0.8 1,736,711 - Pf1N1B4_1502 0.85 -2.4 1,736,711 - Pf1N1B4_1502 0.85 -0.8 1,736,711 - Pf1N1B4_1502 0.85 -0.6 1,736,711 - Pf1N1B4_1502 0.85 +0.1 1,736,711 - Pf1N1B4_1502 0.85 -0.9 1,736,711 - Pf1N1B4_1502 0.85 -1.0 1,736,772 + +2.1 1,736,772 + -1.8 1,736,773 - -1.5 1,736,773 - -1.5 1,736,773 - +1.6 1,736,773 - -0.1 1,736,784 + +0.7 1,736,784 + +0.6 1,736,784 + +0.3 1,736,784 + +1.7 1,736,785 - +0.3 1,736,785 - +0.6 1,736,785 - +1.3 1,736,785 - -0.4 1,736,785 - +0.5 1,736,785 - +0.3 1,736,785 - -1.9 1,736,785 - -1.0 1,736,789 + +0.9 1,736,789 + -0.8 1,736,790 - -0.2 1,736,790 - +0.9 1,736,790 - +1.1 1,736,790 - -1.2 1,736,793 - +1.7 1,736,972 - Pf1N1B4_1503 0.62 +0.2 1,737,010 - Pf1N1B4_1503 0.77 -0.0 1,737,175 + Pf1N1B4_1504 0.38 -0.1 1,737,176 - Pf1N1B4_1504 0.38 -2.1 1,737,176 - Pf1N1B4_1504 0.38 -0.1 1,737,280 - Pf1N1B4_1504 0.79 -1.3 1,737,375 + -0.7 1,737,376 - -0.2 1,737,376 - -0.1 1,737,376 - +0.3 1,737,390 + Pf1N1B4_1505 0.11 +0.6 1,737,390 + Pf1N1B4_1505 0.11 +1.3 1,737,391 - Pf1N1B4_1505 0.11 -0.1 1,737,391 - Pf1N1B4_1505 0.11 -0.3 1,737,618 + Pf1N1B4_1505 0.53 -0.4 1,737,618 + Pf1N1B4_1505 0.53 +0.3 1,737,619 - Pf1N1B4_1505 0.53 +0.3 1,737,668 - Pf1N1B4_1505 0.62 -0.2 1,737,789 + Pf1N1B4_1505 0.84 +0.8 1,737,789 + Pf1N1B4_1505 0.84 +0.7 1,737,789 + Pf1N1B4_1505 0.84 +1.2 1,737,789 + Pf1N1B4_1505 0.84 +1.1 1,737,789 + Pf1N1B4_1505 0.84 +0.3 1,737,789 + Pf1N1B4_1505 0.84 +0.9 1,737,790 - Pf1N1B4_1505 0.84 -2.6 1,737,790 - Pf1N1B4_1505 0.84 +0.0 1,737,790 - Pf1N1B4_1505 0.84 -0.9 1,737,807 + Pf1N1B4_1505 0.87 +0.9 1,737,807 + Pf1N1B4_1505 0.87 +0.5 1,737,808 - Pf1N1B4_1505 0.88 -0.4 1,737,860 - +0.2 1,738,089 + -1.3 1,738,089 + +0.6 1,738,089 + +1.3 1,738,090 - +0.0
Or see this region's nucleotide sequence