Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides Pf1N1B4_1352 and Pf1N1B4_1353 are separated by 126 nucleotides Pf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotides
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135
_1354
Position (kb)
1547
1548
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1546.939 kb on - strand at 1547.087 kb on + strand, within Pf1N1B4_1351 at 1547.088 kb on - strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.325 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand at 1547.448 kb on - strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.593 kb on + strand, within Pf1N1B4_1352 at 1547.782 kb on + strand at 1547.933 kb on - strand at 1548.023 kb on - strand at 1548.309 kb on + strand, within Pf1N1B4_1353 at 1548.718 kb on + strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.785 kb on + strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1549.114 kb on + strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.233 kb on - strand, within Pf1N1B4_1353 at 1549.495 kb on + strand at 1549.496 kb on - strand at 1549.496 kb on - strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.529 kb on - strand at 1549.529 kb on - strand at 1549.556 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.598 kb on - strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.621 kb on - strand at 1549.736 kb on - strand at 1549.739 kb on + strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.791 kb on + strand at 1549.919 kb on + strand at 1549.919 kb on + strand at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,546,939 - -1.4 1,547,087 + Pf1N1B4_1351 0.27 -1.5 1,547,088 - Pf1N1B4_1351 0.27 +1.3 1,547,324 + Pf1N1B4_1351 0.71 -1.7 1,547,324 + Pf1N1B4_1351 0.71 -1.3 1,547,324 + Pf1N1B4_1351 0.71 -1.3 1,547,325 - Pf1N1B4_1351 0.72 -0.7 1,547,364 - Pf1N1B4_1351 0.79 +0.5 1,547,364 - Pf1N1B4_1351 0.79 -0.3 1,547,364 - Pf1N1B4_1351 0.79 -0.1 1,547,364 - +0.3 1,547,448 - +0.3 1,547,450 + +0.3 1,547,450 + -0.7 1,547,450 + +1.3 1,547,451 - +1.9 1,547,451 - -1.3 1,547,451 - +0.4 1,547,451 - +0.9 1,547,593 + Pf1N1B4_1352 0.38 +1.6 1,547,782 + +0.1 1,547,933 - -0.3 1,548,023 - +0.4 1,548,309 + Pf1N1B4_1353 0.23 +1.7 1,548,718 + Pf1N1B4_1353 0.47 +0.7 1,548,719 - Pf1N1B4_1353 0.48 +0.5 1,548,719 - Pf1N1B4_1353 0.48 +0.8 1,548,785 + Pf1N1B4_1353 0.51 +0.5 1,548,786 - Pf1N1B4_1353 0.51 -0.3 1,548,786 - Pf1N1B4_1353 0.51 -0.0 1,548,786 - Pf1N1B4_1353 0.51 -0.3 1,549,114 + Pf1N1B4_1353 0.71 +1.1 1,549,115 - Pf1N1B4_1353 0.71 +1.9 1,549,115 - Pf1N1B4_1353 0.71 -0.7 1,549,126 + Pf1N1B4_1353 0.71 -0.5 1,549,126 + Pf1N1B4_1353 0.71 +0.7 1,549,126 + Pf1N1B4_1353 0.71 -1.3 1,549,126 + Pf1N1B4_1353 0.71 -1.7 1,549,233 - Pf1N1B4_1353 0.78 +1.7 1,549,495 + -1.9 1,549,496 - +0.1 1,549,496 - +1.5 1,549,528 + +1.5 1,549,528 + -1.3 1,549,528 + +1.0 1,549,528 + -0.0 1,549,528 + -0.3 1,549,529 - +1.7 1,549,529 - +0.3 1,549,556 + +0.6 1,549,597 + -1.3 1,549,597 + +0.2 1,549,597 + +1.9 1,549,598 - +0.3 1,549,620 + -2.3 1,549,620 + -1.2 1,549,620 + +0.3 1,549,621 - +1.0 1,549,736 - -0.0 1,549,739 + -0.3 1,549,740 - -0.2 1,549,740 - +0.3 1,549,740 - +0.2 1,549,740 - +0.9 1,549,740 - -0.3 1,549,740 - -0.5 1,549,740 - +1.3 1,549,740 - -0.3 1,549,740 - +1.1 1,549,791 + +1.3 1,549,919 + -0.4 1,549,919 + +0.1 1,549,935 - Pf1N1B4_1354 0.10 +0.3 1,549,935 - Pf1N1B4_1354 0.10 +1.2 1,550,179 - Pf1N1B4_1354 0.20 +0.5 1,550,179 - Pf1N1B4_1354 0.20 -0.1 1,550,374 - Pf1N1B4_1354 0.28 -2.0 1,550,374 - Pf1N1B4_1354 0.28 +0.3 1,550,374 - Pf1N1B4_1354 0.28 -0.0 1,550,374 - Pf1N1B4_1354 0.28 -1.0 1,550,374 - Pf1N1B4_1354 0.28 +0.1
Or see this region's nucleotide sequence