Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1349 and Pf1N1B4_1350 are separated by 9 nucleotides Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides Pf1N1B4_1352 and Pf1N1B4_1353 are separated by 126 nucleotides
Pf1N1B4_1349 - Urease gamma subunit (EC 3.5.1.5), at 1,546,092 to 1,546,394
_1349
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1545.978 kb on - strand at 1545.978 kb on - strand at 1546.047 kb on - strand at 1546.067 kb on - strand at 1546.204 kb on - strand, within Pf1N1B4_1349 at 1546.410 kb on + strand at 1546.410 kb on + strand at 1546.411 kb on - strand at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.462 kb on - strand, within Pf1N1B4_1350 at 1546.598 kb on - strand, within Pf1N1B4_1350 at 1546.668 kb on + strand, within Pf1N1B4_1350 at 1546.669 kb on - strand, within Pf1N1B4_1350 at 1546.849 kb on + strand, within Pf1N1B4_1350 at 1546.939 kb on - strand at 1547.087 kb on + strand, within Pf1N1B4_1351 at 1547.088 kb on - strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.325 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand at 1547.448 kb on - strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.593 kb on + strand, within Pf1N1B4_1352 at 1547.782 kb on + strand at 1547.933 kb on - strand at 1548.023 kb on - strand at 1548.309 kb on + strand, within Pf1N1B4_1353
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,545,978 - +0.1 1,545,978 - +0.5 1,546,047 - +1.9 1,546,067 - +0.9 1,546,204 - Pf1N1B4_1349 0.37 +0.5 1,546,410 + +0.6 1,546,410 + +0.4 1,546,411 - +0.3 1,546,461 + Pf1N1B4_1350 0.11 -2.1 1,546,461 + Pf1N1B4_1350 0.11 +0.9 1,546,461 + Pf1N1B4_1350 0.11 +1.7 1,546,461 + Pf1N1B4_1350 0.11 +0.7 1,546,462 - Pf1N1B4_1350 0.11 -0.9 1,546,598 - Pf1N1B4_1350 0.36 +0.9 1,546,668 + Pf1N1B4_1350 0.49 +0.7 1,546,669 - Pf1N1B4_1350 0.50 -1.9 1,546,849 + Pf1N1B4_1350 0.83 +0.6 1,546,939 - -1.4 1,547,087 + Pf1N1B4_1351 0.27 -1.5 1,547,088 - Pf1N1B4_1351 0.27 +1.3 1,547,324 + Pf1N1B4_1351 0.71 -1.7 1,547,324 + Pf1N1B4_1351 0.71 -1.3 1,547,324 + Pf1N1B4_1351 0.71 -1.3 1,547,325 - Pf1N1B4_1351 0.72 -0.7 1,547,364 - Pf1N1B4_1351 0.79 +0.5 1,547,364 - Pf1N1B4_1351 0.79 -0.3 1,547,364 - Pf1N1B4_1351 0.79 -0.1 1,547,364 - +0.3 1,547,448 - +0.3 1,547,450 + +0.3 1,547,450 + -0.7 1,547,450 + +1.3 1,547,451 - +1.9 1,547,451 - -1.3 1,547,451 - +0.4 1,547,451 - +0.9 1,547,593 + Pf1N1B4_1352 0.38 +1.6 1,547,782 + +0.1 1,547,933 - -0.3 1,548,023 - +0.4 1,548,309 + Pf1N1B4_1353 0.23 +1.7
Or see this region's nucleotide sequence