Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1270 and Pf1N1B4_1271 are separated by 271 nucleotides Pf1N1B4_1271 and Pf1N1B4_1272 are separated by 2 nucleotides
Pf1N1B4_1270 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 1,451,460 to 1,452,737
_1270
Pf1N1B4_1271 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,453,009 to 1,454,361
_1271
Pf1N1B4_1272 - Acyl dehydratase, at 1,454,364 to 1,455,218
_1272
Position (kb)
1453
1454
1455 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1452.366 kb on + strand, within Pf1N1B4_1270 at 1452.366 kb on + strand, within Pf1N1B4_1270 at 1452.367 kb on - strand, within Pf1N1B4_1270 at 1452.484 kb on + strand, within Pf1N1B4_1270 at 1452.484 kb on + strand, within Pf1N1B4_1270 at 1452.485 kb on - strand, within Pf1N1B4_1270 at 1452.485 kb on - strand, within Pf1N1B4_1270 at 1452.485 kb on - strand, within Pf1N1B4_1270 at 1452.485 kb on - strand, within Pf1N1B4_1270 at 1452.553 kb on + strand, within Pf1N1B4_1270 at 1452.553 kb on + strand, within Pf1N1B4_1270 at 1452.553 kb on + strand, within Pf1N1B4_1270 at 1452.554 kb on - strand at 1452.554 kb on - strand, within Pf1N1B4_1270 at 1452.605 kb on + strand, within Pf1N1B4_1270 at 1452.606 kb on - strand, within Pf1N1B4_1270 at 1452.607 kb on + strand, within Pf1N1B4_1270 at 1452.607 kb on + strand, within Pf1N1B4_1270 at 1452.607 kb on + strand, within Pf1N1B4_1270 at 1452.608 kb on - strand, within Pf1N1B4_1270 at 1452.695 kb on + strand at 1452.696 kb on - strand at 1452.696 kb on - strand at 1452.752 kb on + strand at 1452.753 kb on - strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.760 kb on + strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.761 kb on - strand at 1452.893 kb on + strand at 1452.894 kb on - strand at 1452.930 kb on + strand at 1452.930 kb on + strand at 1452.931 kb on - strand at 1452.937 kb on + strand at 1452.937 kb on + strand at 1452.938 kb on - strand at 1452.957 kb on + strand at 1452.958 kb on - strand at 1452.958 kb on - strand at 1452.984 kb on - strand at 1452.984 kb on - strand at 1453.021 kb on + strand at 1453.021 kb on + strand at 1453.022 kb on - strand at 1453.022 kb on - strand at 1453.022 kb on - strand at 1453.022 kb on - strand at 1453.022 kb on - strand at 1453.022 kb on - strand at 1453.207 kb on + strand, within Pf1N1B4_1271 at 1453.207 kb on + strand, within Pf1N1B4_1271 at 1453.207 kb on + strand, within Pf1N1B4_1271 at 1453.282 kb on + strand, within Pf1N1B4_1271 at 1453.282 kb on + strand, within Pf1N1B4_1271 at 1453.282 kb on + strand, within Pf1N1B4_1271 at 1453.282 kb on + strand, within Pf1N1B4_1271 at 1453.283 kb on - strand, within Pf1N1B4_1271 at 1453.283 kb on - strand, within Pf1N1B4_1271 at 1453.379 kb on + strand, within Pf1N1B4_1271 at 1453.379 kb on + strand, within Pf1N1B4_1271 at 1453.380 kb on - strand at 1453.380 kb on - strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.504 kb on + strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.505 kb on - strand, within Pf1N1B4_1271 at 1453.970 kb on + strand, within Pf1N1B4_1271 at 1453.970 kb on + strand, within Pf1N1B4_1271 at 1453.971 kb on - strand, within Pf1N1B4_1271 at 1453.971 kb on - strand, within Pf1N1B4_1271 at 1454.074 kb on + strand, within Pf1N1B4_1271 at 1454.074 kb on + strand, within Pf1N1B4_1271 at 1454.074 kb on + strand, within Pf1N1B4_1271 at 1454.075 kb on - strand, within Pf1N1B4_1271 at 1454.415 kb on + strand at 1454.415 kb on + strand at 1454.415 kb on + strand at 1454.415 kb on + strand at 1454.415 kb on + strand at 1454.416 kb on - strand at 1454.416 kb on - strand at 1454.416 kb on - strand at 1454.416 kb on - strand at 1454.416 kb on - strand at 1454.551 kb on + strand, within Pf1N1B4_1272 at 1454.551 kb on + strand, within Pf1N1B4_1272 at 1454.562 kb on + strand, within Pf1N1B4_1272 at 1454.562 kb on + strand, within Pf1N1B4_1272 at 1454.563 kb on - strand at 1454.563 kb on - strand, within Pf1N1B4_1272 at 1454.563 kb on - strand, within Pf1N1B4_1272 at 1454.665 kb on + strand, within Pf1N1B4_1272 at 1454.943 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.029 kb on + strand, within Pf1N1B4_1272 at 1455.030 kb on - strand, within Pf1N1B4_1272 at 1455.030 kb on - strand, within Pf1N1B4_1272 at 1455.030 kb on - strand, within Pf1N1B4_1272 at 1455.030 kb on - strand, within Pf1N1B4_1272 at 1455.188 kb on + strand at 1455.188 kb on + strand at 1455.188 kb on + strand at 1455.189 kb on - strand at 1455.189 kb on - strand at 1455.189 kb on - strand at 1455.231 kb on + strand at 1455.231 kb on + strand at 1455.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,452,366 + Pf1N1B4_1270 0.71 -2.6 1,452,366 + Pf1N1B4_1270 0.71 -1.1 1,452,367 - Pf1N1B4_1270 0.71 -0.8 1,452,484 + Pf1N1B4_1270 0.80 -0.3 1,452,484 + Pf1N1B4_1270 0.80 +1.2 1,452,485 - Pf1N1B4_1270 0.80 +0.2 1,452,485 - Pf1N1B4_1270 0.80 +0.2 1,452,485 - Pf1N1B4_1270 0.80 +0.5 1,452,485 - Pf1N1B4_1270 0.80 +0.8 1,452,553 + Pf1N1B4_1270 0.86 +0.8 1,452,553 + Pf1N1B4_1270 0.86 +1.2 1,452,553 + Pf1N1B4_1270 0.86 -1.8 1,452,554 - +1.6 1,452,554 - Pf1N1B4_1270 0.86 -0.5 1,452,605 + Pf1N1B4_1270 0.90 -1.1 1,452,606 - Pf1N1B4_1270 0.90 +0.2 1,452,607 + Pf1N1B4_1270 0.90 +1.0 1,452,607 + Pf1N1B4_1270 0.90 -0.8 1,452,607 + Pf1N1B4_1270 0.90 -0.1 1,452,608 - Pf1N1B4_1270 0.90 -0.8 1,452,695 + +0.6 1,452,696 - +1.2 1,452,696 - +0.5 1,452,752 + +1.0 1,452,753 - -1.1 1,452,760 + -0.0 1,452,760 + +0.0 1,452,760 + -0.0 1,452,760 + -0.2 1,452,760 + +1.2 1,452,760 + +0.9 1,452,760 + -2.1 1,452,760 + -1.1 1,452,761 - -2.2 1,452,761 - -0.4 1,452,761 - -0.8 1,452,761 - +0.6 1,452,761 - -2.1 1,452,761 - -2.1 1,452,761 - -1.8 1,452,893 + +0.8 1,452,894 - -1.1 1,452,930 + -0.8 1,452,930 + -1.1 1,452,931 - -2.2 1,452,937 + +0.2 1,452,937 + -1.8 1,452,938 - -0.8 1,452,957 + -0.1 1,452,958 - -2.2 1,452,958 - -3.0 1,452,984 - -0.8 1,452,984 - -0.3 1,453,021 + -1.1 1,453,021 + +0.2 1,453,022 - -2.1 1,453,022 - -0.8 1,453,022 - -0.8 1,453,022 - -3.0 1,453,022 - -2.5 1,453,022 - -1.1 1,453,207 + Pf1N1B4_1271 0.15 -0.4 1,453,207 + Pf1N1B4_1271 0.15 +0.4 1,453,207 + Pf1N1B4_1271 0.15 -0.6 1,453,282 + Pf1N1B4_1271 0.20 -0.3 1,453,282 + Pf1N1B4_1271 0.20 +0.4 1,453,282 + Pf1N1B4_1271 0.20 +0.0 1,453,282 + Pf1N1B4_1271 0.20 +1.5 1,453,283 - Pf1N1B4_1271 0.20 -1.1 1,453,283 - Pf1N1B4_1271 0.20 -1.8 1,453,379 + Pf1N1B4_1271 0.27 -0.9 1,453,379 + Pf1N1B4_1271 0.27 -0.2 1,453,380 - -0.8 1,453,380 - Pf1N1B4_1271 0.27 -1.1 1,453,504 + Pf1N1B4_1271 0.37 -1.4 1,453,504 + Pf1N1B4_1271 0.37 -1.4 1,453,504 + Pf1N1B4_1271 0.37 -0.4 1,453,504 + Pf1N1B4_1271 0.37 +2.0 1,453,504 + Pf1N1B4_1271 0.37 -1.6 1,453,504 + Pf1N1B4_1271 0.37 -0.1 1,453,504 + Pf1N1B4_1271 0.37 -0.1 1,453,505 - Pf1N1B4_1271 0.37 -0.8 1,453,505 - Pf1N1B4_1271 0.37 +1.2 1,453,505 - Pf1N1B4_1271 0.37 -2.1 1,453,505 - Pf1N1B4_1271 0.37 -0.1 1,453,505 - Pf1N1B4_1271 0.37 -2.0 1,453,505 - Pf1N1B4_1271 0.37 -1.4 1,453,505 - Pf1N1B4_1271 0.37 -1.4 1,453,505 - Pf1N1B4_1271 0.37 -1.6 1,453,970 + Pf1N1B4_1271 0.71 -1.1 1,453,970 + Pf1N1B4_1271 0.71 -0.2 1,453,971 - Pf1N1B4_1271 0.71 -2.2 1,453,971 - Pf1N1B4_1271 0.71 -2.4 1,454,074 + Pf1N1B4_1271 0.79 -1.5 1,454,074 + Pf1N1B4_1271 0.79 +0.2 1,454,074 + Pf1N1B4_1271 0.79 +1.2 1,454,075 - Pf1N1B4_1271 0.79 +0.2 1,454,415 + -1.8 1,454,415 + -0.2 1,454,415 + -1.1 1,454,415 + -1.0 1,454,415 + +1.8 1,454,416 - -0.4 1,454,416 - -2.2 1,454,416 - -2.0 1,454,416 - -1.1 1,454,416 - -1.1 1,454,551 + Pf1N1B4_1272 0.22 -1.4 1,454,551 + Pf1N1B4_1272 0.22 +0.5 1,454,562 + Pf1N1B4_1272 0.23 +0.9 1,454,562 + Pf1N1B4_1272 0.23 +1.2 1,454,563 - +0.2 1,454,563 - Pf1N1B4_1272 0.23 +0.2 1,454,563 - Pf1N1B4_1272 0.23 -2.2 1,454,665 + Pf1N1B4_1272 0.35 -0.8 1,454,943 + Pf1N1B4_1272 0.68 +0.6 1,455,029 + Pf1N1B4_1272 0.78 +0.1 1,455,029 + Pf1N1B4_1272 0.78 -1.1 1,455,029 + Pf1N1B4_1272 0.78 +0.9 1,455,029 + Pf1N1B4_1272 0.78 -0.2 1,455,029 + Pf1N1B4_1272 0.78 -0.4 1,455,029 + Pf1N1B4_1272 0.78 -0.6 1,455,030 - Pf1N1B4_1272 0.78 -0.4 1,455,030 - Pf1N1B4_1272 0.78 +0.8 1,455,030 - Pf1N1B4_1272 0.78 -1.4 1,455,030 - Pf1N1B4_1272 0.78 -0.4 1,455,188 + +1.6 1,455,188 + -1.8 1,455,188 + +1.2 1,455,189 - -1.1 1,455,189 - +1.2 1,455,189 - +0.6 1,455,231 + +0.6 1,455,231 + +0.3 1,455,232 - -0.1
Or see this region's nucleotide sequence