Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_126 and Pf1N1B4_127 are separated by 105 nucleotides Pf1N1B4_127 and Pf1N1B4_128 are separated by 254 nucleotides
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Pf1N1B4_127 - FIG032225: Transcriptional regulator, LysR family, at 138,939 to 139,847
_127
Pf1N1B4_128 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 140,102 to 140,905
_128
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 137.955 kb on - strand at 137.955 kb on - strand at 138.289 kb on - strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.811 kb on + strand at 138.926 kb on + strand at 138.964 kb on - strand at 138.966 kb on + strand at 138.967 kb on - strand at 139.134 kb on + strand, within Pf1N1B4_127 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.294 kb on - strand, within Pf1N1B4_127 at 139.498 kb on + strand, within Pf1N1B4_127 at 139.542 kb on + strand, within Pf1N1B4_127 at 140.007 kb on + strand at 140.063 kb on + strand at 140.129 kb on - strand at 140.521 kb on + strand, within Pf1N1B4_128 at 140.522 kb on - strand, within Pf1N1B4_128 at 140.574 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 137,955 - +0.6 137,955 - +1.6 138,289 - Pf1N1B4_126 0.37 -1.2 138,616 + Pf1N1B4_126 0.75 -1.1 138,616 + Pf1N1B4_126 0.75 -0.2 138,616 + Pf1N1B4_126 0.75 -1.7 138,811 + -1.6 138,926 + +2.6 138,964 - -1.6 138,966 + -1.8 138,967 - +0.8 139,134 + Pf1N1B4_127 0.21 +0.3 139,134 + Pf1N1B4_127 0.21 +1.2 139,281 + Pf1N1B4_127 0.38 +0.8 139,281 + Pf1N1B4_127 0.38 +0.8 139,282 - Pf1N1B4_127 0.38 +0.8 139,282 - Pf1N1B4_127 0.38 +2.1 139,282 - Pf1N1B4_127 0.38 -0.2 139,282 - Pf1N1B4_127 0.38 -0.5 139,293 + Pf1N1B4_127 0.39 +2.0 139,293 + Pf1N1B4_127 0.39 -2.2 139,294 - Pf1N1B4_127 0.39 +2.6 139,498 + Pf1N1B4_127 0.61 +2.3 139,542 + Pf1N1B4_127 0.66 +1.9 140,007 + -0.2 140,063 + -0.5 140,129 - +0.5 140,521 + Pf1N1B4_128 0.52 -0.2 140,522 - Pf1N1B4_128 0.52 +0.2 140,574 - Pf1N1B4_128 0.59 +2.1 140,634 - Pf1N1B4_128 0.66 +0.2 140,634 - Pf1N1B4_128 0.66 -1.2 140,634 - Pf1N1B4_128 0.66 +1.5 140,634 - Pf1N1B4_128 0.66 -1.1
Or see this region's nucleotide sequence