Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_121 and Pf1N1B4_122 overlap by 4 nucleotides Pf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotides Pf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides
Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293
_121
Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882
_122
Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332
_123
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.350 kb on - strand, within Pf1N1B4_121 at 132.524 kb on + strand, within Pf1N1B4_121 at 132.641 kb on - strand, within Pf1N1B4_121 at 132.856 kb on + strand, within Pf1N1B4_121 at 132.968 kb on + strand, within Pf1N1B4_121 at 133.429 kb on + strand at 133.833 kb on + strand, within Pf1N1B4_122 at 133.834 kb on - strand, within Pf1N1B4_122 at 133.930 kb on - strand, within Pf1N1B4_122 at 133.975 kb on - strand, within Pf1N1B4_122 at 134.187 kb on + strand, within Pf1N1B4_122 at 134.293 kb on - strand, within Pf1N1B4_122 at 134.675 kb on + strand, within Pf1N1B4_122 at 134.676 kb on - strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.773 kb on - strand at 134.894 kb on - strand at 135.011 kb on - strand, within Pf1N1B4_123 at 135.011 kb on - strand, within Pf1N1B4_123 at 135.311 kb on - strand at 135.389 kb on - strand at 135.389 kb on - strand at 135.394 kb on + strand at 135.403 kb on - strand at 135.403 kb on - strand at 135.425 kb on + strand at 135.526 kb on + strand at 135.735 kb on + strand, within Pf1N1B4_124
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 132,349 + Pf1N1B4_121 0.16 +0.8 132,349 + Pf1N1B4_121 0.16 +0.3 132,350 - Pf1N1B4_121 0.16 -1.1 132,524 + Pf1N1B4_121 0.31 -1.2 132,641 - Pf1N1B4_121 0.42 -0.2 132,856 + Pf1N1B4_121 0.61 -1.2 132,968 + Pf1N1B4_121 0.71 -0.1 133,429 + +1.4 133,833 + Pf1N1B4_122 0.34 -0.8 133,834 - Pf1N1B4_122 0.34 +0.6 133,930 - Pf1N1B4_122 0.40 -1.1 133,975 - Pf1N1B4_122 0.43 -2.4 134,187 + Pf1N1B4_122 0.56 -0.0 134,293 - Pf1N1B4_122 0.63 -1.6 134,675 + Pf1N1B4_122 0.87 -0.8 134,676 - +2.3 134,772 + +0.4 134,772 + -0.2 134,772 + -0.8 134,773 - +1.3 134,894 - +1.9 135,011 - Pf1N1B4_123 0.27 -0.8 135,011 - Pf1N1B4_123 0.27 +0.9 135,311 - -0.2 135,389 - +0.5 135,389 - -0.6 135,394 + +1.6 135,403 - -0.2 135,403 - +0.4 135,425 + -0.7 135,526 + -1.4 135,735 + Pf1N1B4_124 0.11 -1.1
Or see this region's nucleotide sequence