Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1090 and Pf1N1B4_1091 are separated by 109 nucleotides Pf1N1B4_1091 and Pf1N1B4_1092 are separated by 48 nucleotides
Pf1N1B4_1090 - TldE protein, part of TldE/TldD proteolytic complex, at 1,219,777 to 1,221,123
_1090
Pf1N1B4_1091 - FIG138315: Putative alpha helix protein, at 1,221,233 to 1,221,757
_1091
Pf1N1B4_1092 - TldD protein, part of TldE/TldD proteolytic complex, at 1,221,806 to 1,223,248
_1092
Position (kb)
1221
1222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1220.510 kb on + strand, within Pf1N1B4_1090 at 1220.511 kb on - strand, within Pf1N1B4_1090 at 1220.517 kb on - strand, within Pf1N1B4_1090 at 1220.596 kb on - strand, within Pf1N1B4_1090 at 1220.914 kb on + strand, within Pf1N1B4_1090 at 1220.915 kb on - strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.331 kb on + strand at 1221.331 kb on + strand at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.380 kb on - strand, within Pf1N1B4_1091 at 1221.380 kb on - strand, within Pf1N1B4_1091 at 1221.421 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.527 kb on + strand, within Pf1N1B4_1091 at 1221.527 kb on + strand, within Pf1N1B4_1091 at 1221.528 kb on - strand, within Pf1N1B4_1091 at 1221.716 kb on + strand at 1221.716 kb on + strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.808 kb on - strand at 1221.829 kb on + strand at 1221.830 kb on - strand at 1222.023 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.096 kb on - strand, within Pf1N1B4_1092 at 1222.096 kb on - strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.192 kb on - strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.261 kb on - strand, within Pf1N1B4_1092 at 1222.578 kb on + strand, within Pf1N1B4_1092 at 1222.609 kb on - strand, within Pf1N1B4_1092
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,220,510 + Pf1N1B4_1090 0.54 -0.3 1,220,511 - Pf1N1B4_1090 0.54 -0.2 1,220,517 - Pf1N1B4_1090 0.55 -1.2 1,220,596 - Pf1N1B4_1090 0.61 -1.4 1,220,914 + Pf1N1B4_1090 0.84 -0.1 1,220,915 - Pf1N1B4_1090 0.84 +0.9 1,220,918 + Pf1N1B4_1090 0.85 +0.9 1,220,918 + Pf1N1B4_1090 0.85 +0.1 1,220,918 + Pf1N1B4_1090 0.85 -1.9 1,220,919 - Pf1N1B4_1090 0.85 +1.1 1,220,919 - Pf1N1B4_1090 0.85 +1.8 1,220,919 - +1.1 1,220,919 - Pf1N1B4_1090 0.85 -0.4 1,220,919 - Pf1N1B4_1090 0.85 +0.1 1,220,919 - Pf1N1B4_1090 0.85 -1.4 1,220,919 - Pf1N1B4_1090 0.85 +1.1 1,220,919 - Pf1N1B4_1090 0.85 +0.1 1,220,919 - Pf1N1B4_1090 0.85 +0.8 1,220,919 - Pf1N1B4_1090 0.85 -1.0 1,221,331 + Pf1N1B4_1091 0.19 +1.5 1,221,331 + -0.1 1,221,331 + +1.6 1,221,331 + Pf1N1B4_1091 0.19 -0.2 1,221,331 + Pf1N1B4_1091 0.19 +1.7 1,221,380 - Pf1N1B4_1091 0.28 -1.2 1,221,380 - Pf1N1B4_1091 0.28 +0.0 1,221,421 + Pf1N1B4_1091 0.36 +1.5 1,221,442 + Pf1N1B4_1091 0.40 +1.1 1,221,442 + Pf1N1B4_1091 0.40 -0.9 1,221,442 + Pf1N1B4_1091 0.40 +0.4 1,221,442 + Pf1N1B4_1091 0.40 -1.6 1,221,442 + Pf1N1B4_1091 0.40 -1.4 1,221,442 + +1.1 1,221,442 + Pf1N1B4_1091 0.40 -1.2 1,221,442 + Pf1N1B4_1091 0.40 +1.0 1,221,442 + Pf1N1B4_1091 0.40 +1.1 1,221,442 + Pf1N1B4_1091 0.40 -0.2 1,221,442 + Pf1N1B4_1091 0.40 +0.9 1,221,442 + Pf1N1B4_1091 0.40 -1.7 1,221,442 + Pf1N1B4_1091 0.40 +2.5 1,221,443 - Pf1N1B4_1091 0.40 -0.1 1,221,443 - Pf1N1B4_1091 0.40 +1.6 1,221,443 - Pf1N1B4_1091 0.40 +1.1 1,221,443 - Pf1N1B4_1091 0.40 -0.1 1,221,527 + Pf1N1B4_1091 0.56 +2.1 1,221,527 + Pf1N1B4_1091 0.56 +2.5 1,221,528 - Pf1N1B4_1091 0.56 +1.6 1,221,716 + +0.1 1,221,716 + -0.3 1,221,717 - +1.1 1,221,717 - +0.4 1,221,717 - -0.7 1,221,717 - -0.2 1,221,717 - -0.9 1,221,717 - +0.1 1,221,808 - +1.1 1,221,829 + +0.9 1,221,830 - +1.6 1,222,023 + Pf1N1B4_1092 0.15 +1.1 1,222,095 + Pf1N1B4_1092 0.20 +0.3 1,222,095 + Pf1N1B4_1092 0.20 +0.9 1,222,095 + Pf1N1B4_1092 0.20 -0.4 1,222,095 + Pf1N1B4_1092 0.20 +0.5 1,222,095 + Pf1N1B4_1092 0.20 +0.0 1,222,096 - Pf1N1B4_1092 0.20 +0.5 1,222,096 - Pf1N1B4_1092 0.20 -0.9 1,222,155 + Pf1N1B4_1092 0.24 -0.4 1,222,155 + Pf1N1B4_1092 0.24 -0.2 1,222,155 + Pf1N1B4_1092 0.24 +0.3 1,222,155 + Pf1N1B4_1092 0.24 +0.3 1,222,155 + Pf1N1B4_1092 0.24 +0.8 1,222,155 + Pf1N1B4_1092 0.24 -1.2 1,222,155 + Pf1N1B4_1092 0.24 +0.2 1,222,155 + Pf1N1B4_1092 0.24 -0.9 1,222,156 - Pf1N1B4_1092 0.24 -2.0 1,222,156 - Pf1N1B4_1092 0.24 -0.4 1,222,156 - Pf1N1B4_1092 0.24 +1.7 1,222,156 - Pf1N1B4_1092 0.24 -0.3 1,222,156 - Pf1N1B4_1092 0.24 +1.5 1,222,156 - Pf1N1B4_1092 0.24 -0.2 1,222,156 - Pf1N1B4_1092 0.24 +0.1 1,222,156 - Pf1N1B4_1092 0.24 +0.9 1,222,156 - Pf1N1B4_1092 0.24 -0.2 1,222,156 - +2.1 1,222,156 - Pf1N1B4_1092 0.24 -0.4 1,222,156 - Pf1N1B4_1092 0.24 +0.7 1,222,192 - Pf1N1B4_1092 0.27 +0.1 1,222,258 + Pf1N1B4_1092 0.31 +1.1 1,222,258 + Pf1N1B4_1092 0.31 +0.7 1,222,258 + Pf1N1B4_1092 0.31 -1.2 1,222,258 + Pf1N1B4_1092 0.31 +0.1 1,222,258 + Pf1N1B4_1092 0.31 +0.1 1,222,259 - Pf1N1B4_1092 0.31 -1.4 1,222,259 - Pf1N1B4_1092 0.31 -0.9 1,222,259 - Pf1N1B4_1092 0.31 +0.1 1,222,259 - Pf1N1B4_1092 0.31 +0.1 1,222,259 - Pf1N1B4_1092 0.31 -0.4 1,222,259 - Pf1N1B4_1092 0.31 -0.3 1,222,259 - Pf1N1B4_1092 0.31 +1.6 1,222,259 - +0.1 1,222,260 + Pf1N1B4_1092 0.31 +0.1 1,222,260 + Pf1N1B4_1092 0.31 +1.0 1,222,260 + Pf1N1B4_1092 0.31 -0.9 1,222,260 + Pf1N1B4_1092 0.31 -0.2 1,222,260 + Pf1N1B4_1092 0.31 -0.7 1,222,260 + Pf1N1B4_1092 0.31 +1.1 1,222,261 - Pf1N1B4_1092 0.32 -0.1 1,222,578 + Pf1N1B4_1092 0.53 +0.1 1,222,609 - Pf1N1B4_1092 0.56 +0.7
Or see this region's nucleotide sequence