Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1053 and Pf1N1B4_1054 are separated by 15 nucleotides Pf1N1B4_1054 and Pf1N1B4_1055 overlap by 4 nucleotides Pf1N1B4_1055 and Pf1N1B4_1056 are separated by 0 nucleotides
Pf1N1B4_1053 - hypothetical protein, at 1,188,680 to 1,188,991
_1053
Pf1N1B4_1054 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,007 to 1,189,840
_1054
Pf1N1B4_1055 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,837 to 1,191,105
_1055
Pf1N1B4_1056 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,191,106 to 1,192,014
_1056
Position (kb)
1189
1190
1191
1192 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.968 kb on + strand at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054 at 1189.730 kb on - strand, within Pf1N1B4_1054 at 1189.753 kb on + strand, within Pf1N1B4_1054 at 1189.753 kb on + strand, within Pf1N1B4_1054 at 1189.753 kb on + strand, within Pf1N1B4_1054 at 1189.963 kb on + strand at 1189.964 kb on - strand, within Pf1N1B4_1055 at 1190.353 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.386 kb on - strand, within Pf1N1B4_1055 at 1190.386 kb on - strand, within Pf1N1B4_1055 at 1190.605 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.831 kb on - strand, within Pf1N1B4_1055 at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.053 kb on - strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.103 kb on + strand at 1191.103 kb on + strand at 1191.119 kb on + strand at 1191.359 kb on - strand, within Pf1N1B4_1056 at 1191.370 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.548 kb on - strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.901 kb on + strand, within Pf1N1B4_1056 at 1191.902 kb on - strand, within Pf1N1B4_1056 at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,188,871 + Pf1N1B4_1053 0.61 +2.2 1,188,871 + Pf1N1B4_1053 0.61 +0.2 1,188,968 + -1.0 1,189,283 + Pf1N1B4_1054 0.33 -1.2 1,189,283 + Pf1N1B4_1054 0.33 -0.2 1,189,352 + Pf1N1B4_1054 0.41 +0.2 1,189,352 + Pf1N1B4_1054 0.41 +0.4 1,189,730 - Pf1N1B4_1054 0.87 -1.7 1,189,753 + Pf1N1B4_1054 0.89 -0.8 1,189,753 + Pf1N1B4_1054 0.89 +1.3 1,189,753 + Pf1N1B4_1054 0.89 -1.2 1,189,963 + +0.8 1,189,964 - Pf1N1B4_1055 0.10 -0.3 1,190,353 + Pf1N1B4_1055 0.41 +0.1 1,190,385 + Pf1N1B4_1055 0.43 -1.7 1,190,385 + Pf1N1B4_1055 0.43 +0.6 1,190,385 + Pf1N1B4_1055 0.43 -0.4 1,190,385 + Pf1N1B4_1055 0.43 -0.4 1,190,386 - Pf1N1B4_1055 0.43 +1.7 1,190,386 - Pf1N1B4_1055 0.43 -1.2 1,190,605 + Pf1N1B4_1055 0.61 -1.2 1,190,722 + Pf1N1B4_1055 0.70 -0.3 1,190,722 + Pf1N1B4_1055 0.70 -0.4 1,190,722 + -0.7 1,190,722 + Pf1N1B4_1055 0.70 -0.3 1,190,831 - Pf1N1B4_1055 0.78 +0.4 1,191,052 + -0.1 1,191,052 + -0.2 1,191,052 + +0.6 1,191,052 + +1.9 1,191,052 + -0.2 1,191,052 + -1.4 1,191,053 - +0.1 1,191,093 + -0.3 1,191,093 + -2.8 1,191,093 + -0.5 1,191,093 + -0.2 1,191,093 + +2.1 1,191,093 + +0.4 1,191,093 + -0.2 1,191,093 + -0.2 1,191,094 - +0.8 1,191,094 - -0.2 1,191,094 - +0.4 1,191,094 - -0.1 1,191,094 - +2.1 1,191,094 - -1.9 1,191,103 + +0.3 1,191,103 + -0.2 1,191,119 + -0.4 1,191,359 - Pf1N1B4_1056 0.28 -1.7 1,191,370 + Pf1N1B4_1056 0.29 -0.1 1,191,421 + Pf1N1B4_1056 0.35 -0.4 1,191,421 + Pf1N1B4_1056 0.35 -0.3 1,191,421 + Pf1N1B4_1056 0.35 +1.7 1,191,421 + Pf1N1B4_1056 0.35 -1.0 1,191,422 - Pf1N1B4_1056 0.35 +0.0 1,191,422 - Pf1N1B4_1056 0.35 -0.4 1,191,548 - Pf1N1B4_1056 0.49 +0.1 1,191,680 + Pf1N1B4_1056 0.63 -1.2 1,191,680 + Pf1N1B4_1056 0.63 -0.4 1,191,680 + Pf1N1B4_1056 0.63 +0.6 1,191,681 - Pf1N1B4_1056 0.63 +1.1 1,191,681 - Pf1N1B4_1056 0.63 -0.4 1,191,901 + Pf1N1B4_1056 0.87 -1.2 1,191,902 - Pf1N1B4_1056 0.88 +2.0 1,191,997 + -0.2 1,191,997 + -0.4 1,191,997 + +1.0 1,191,998 - +0.8
Or see this region's nucleotide sequence