Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1033 and Pf1N1B4_1034 overlap by 30 nucleotides Pf1N1B4_1034 and Pf1N1B4_1035 overlap by 20 nucleotides Pf1N1B4_1035 and Pf1N1B4_1036 are separated by 103 nucleotides Pf1N1B4_1036 and Pf1N1B4_1037 are separated by 54 nucleotides Pf1N1B4_1037 and Pf1N1B4_1038 are separated by 19 nucleotides
Pf1N1B4_1033 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 1,174,260 to 1,175,042
_1033
Pf1N1B4_1034 - hypothetical protein, at 1,175,013 to 1,175,279
_1034
Pf1N1B4_1035 - Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C), at 1,175,260 to 1,175,715
_1035
Pf1N1B4_1036 - Signal transduction histidine kinase, at 1,175,819 to 1,176,295
_1036
Pf1N1B4_1037 - FIG00953110: hypothetical protein, at 1,176,350 to 1,176,955
_1037
Pf1N1B4_1038 - Transcriptional regulator, MerR family, at 1,176,975 to 1,177,934
_1038
Position (kb)
1175
1176
1177 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1175.095 kb on + strand, within Pf1N1B4_1034 at 1175.105 kb on + strand, within Pf1N1B4_1034 at 1175.105 kb on + strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.127 kb on - strand, within Pf1N1B4_1034 at 1175.127 kb on - strand, within Pf1N1B4_1034 at 1175.127 kb on - strand at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.685 kb on - strand at 1175.685 kb on - strand at 1175.743 kb on + strand at 1175.751 kb on - strand at 1175.779 kb on + strand at 1175.792 kb on - strand at 1175.915 kb on + strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.920 kb on - strand, within Pf1N1B4_1036 at 1175.949 kb on - strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1176.152 kb on + strand, within Pf1N1B4_1036 at 1176.152 kb on + strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.305 kb on - strand at 1176.544 kb on - strand, within Pf1N1B4_1037 at 1176.544 kb on - strand, within Pf1N1B4_1037 at 1176.591 kb on + strand, within Pf1N1B4_1037 at 1176.591 kb on + strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.614 kb on + strand, within Pf1N1B4_1037 at 1176.614 kb on + strand, within Pf1N1B4_1037 at 1176.615 kb on - strand at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.717 kb on + strand, within Pf1N1B4_1037 at 1176.718 kb on - strand, within Pf1N1B4_1037 at 1176.854 kb on + strand, within Pf1N1B4_1037 at 1176.861 kb on - strand, within Pf1N1B4_1037 at 1176.908 kb on + strand at 1176.909 kb on - strand at 1176.909 kb on - strand at 1176.909 kb on - strand at 1176.950 kb on - strand at 1176.950 kb on - strand at 1176.966 kb on + strand at 1176.967 kb on - strand at 1176.967 kb on - strand at 1176.967 kb on - strand at 1176.970 kb on + strand at 1176.970 kb on + strand at 1176.970 kb on + strand at 1176.971 kb on - strand at 1176.971 kb on - strand at 1176.980 kb on + strand at 1177.185 kb on + strand, within Pf1N1B4_1038 at 1177.263 kb on - strand, within Pf1N1B4_1038 at 1177.285 kb on + strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,175,095 + Pf1N1B4_1034 0.31 +0.9 1,175,105 + Pf1N1B4_1034 0.34 -1.1 1,175,105 + Pf1N1B4_1034 0.34 +0.6 1,175,106 - Pf1N1B4_1034 0.35 -0.4 1,175,106 - Pf1N1B4_1034 0.35 -0.5 1,175,106 - Pf1N1B4_1034 0.35 -0.3 1,175,126 + Pf1N1B4_1034 0.42 +0.8 1,175,126 + Pf1N1B4_1034 0.42 +0.4 1,175,126 + Pf1N1B4_1034 0.42 +0.7 1,175,126 + Pf1N1B4_1034 0.42 +0.8 1,175,126 + Pf1N1B4_1034 0.42 -0.9 1,175,126 + Pf1N1B4_1034 0.42 -0.6 1,175,126 + Pf1N1B4_1034 0.42 +0.7 1,175,127 - Pf1N1B4_1034 0.43 +1.1 1,175,127 - Pf1N1B4_1034 0.43 +0.1 1,175,127 - +1.5 1,175,504 + Pf1N1B4_1035 0.54 +1.5 1,175,504 + Pf1N1B4_1035 0.54 +0.4 1,175,504 + +1.1 1,175,504 + Pf1N1B4_1035 0.54 +1.6 1,175,504 + Pf1N1B4_1035 0.54 +1.7 1,175,504 + Pf1N1B4_1035 0.54 +3.1 1,175,505 - Pf1N1B4_1035 0.54 +0.3 1,175,505 - Pf1N1B4_1035 0.54 +1.6 1,175,505 - Pf1N1B4_1035 0.54 +1.5 1,175,505 - Pf1N1B4_1035 0.54 -0.4 1,175,685 - +0.4 1,175,685 - +0.9 1,175,743 + +1.9 1,175,751 - -0.2 1,175,779 + +0.3 1,175,792 - -0.4 1,175,915 + Pf1N1B4_1036 0.20 +0.2 1,175,916 - Pf1N1B4_1036 0.20 -0.8 1,175,916 - Pf1N1B4_1036 0.20 -1.4 1,175,916 - Pf1N1B4_1036 0.20 -0.7 1,175,920 - Pf1N1B4_1036 0.21 -2.4 1,175,949 - Pf1N1B4_1036 0.27 -1.1 1,175,950 + Pf1N1B4_1036 0.27 -1.8 1,175,950 + Pf1N1B4_1036 0.27 -0.3 1,175,950 + Pf1N1B4_1036 0.27 +0.9 1,175,950 + Pf1N1B4_1036 0.27 -0.4 1,175,951 - Pf1N1B4_1036 0.28 -0.7 1,175,951 - Pf1N1B4_1036 0.28 +0.4 1,175,951 - Pf1N1B4_1036 0.28 -0.3 1,175,951 - +2.2 1,175,951 - Pf1N1B4_1036 0.28 -2.0 1,176,152 + Pf1N1B4_1036 0.70 +0.2 1,176,152 + Pf1N1B4_1036 0.70 -1.2 1,176,153 - Pf1N1B4_1036 0.70 -0.4 1,176,153 - Pf1N1B4_1036 0.70 -1.2 1,176,153 - Pf1N1B4_1036 0.70 -1.2 1,176,153 - Pf1N1B4_1036 0.70 -0.6 1,176,153 - Pf1N1B4_1036 0.70 -1.2 1,176,153 - Pf1N1B4_1036 0.70 -0.6 1,176,153 - Pf1N1B4_1036 0.70 -1.4 1,176,305 - -0.8 1,176,544 - Pf1N1B4_1037 0.32 +0.0 1,176,544 - Pf1N1B4_1037 0.32 -1.8 1,176,591 + Pf1N1B4_1037 0.40 -0.4 1,176,591 + Pf1N1B4_1037 0.40 +1.2 1,176,592 - Pf1N1B4_1037 0.40 +0.4 1,176,592 - Pf1N1B4_1037 0.40 +1.0 1,176,592 - Pf1N1B4_1037 0.40 +0.6 1,176,592 - Pf1N1B4_1037 0.40 -0.2 1,176,614 + Pf1N1B4_1037 0.44 -0.0 1,176,614 + Pf1N1B4_1037 0.44 -0.0 1,176,615 - +1.2 1,176,615 - Pf1N1B4_1037 0.44 +0.4 1,176,615 - Pf1N1B4_1037 0.44 -0.4 1,176,615 - Pf1N1B4_1037 0.44 -1.4 1,176,615 - Pf1N1B4_1037 0.44 +0.6 1,176,615 - Pf1N1B4_1037 0.44 -1.7 1,176,615 - Pf1N1B4_1037 0.44 -0.8 1,176,615 - Pf1N1B4_1037 0.44 -0.1 1,176,717 + Pf1N1B4_1037 0.61 +0.7 1,176,718 - Pf1N1B4_1037 0.61 -0.8 1,176,854 + Pf1N1B4_1037 0.83 +1.4 1,176,861 - Pf1N1B4_1037 0.84 -2.4 1,176,908 + -0.2 1,176,909 - +1.3 1,176,909 - +0.8 1,176,909 - +0.6 1,176,950 - -1.7 1,176,950 - -2.4 1,176,966 + +0.7 1,176,967 - +0.2 1,176,967 - +0.5 1,176,967 - -0.1 1,176,970 + -0.5 1,176,970 + +1.8 1,176,970 + +1.5 1,176,971 - -0.4 1,176,971 - -1.2 1,176,980 + +1.0 1,177,185 + Pf1N1B4_1038 0.22 -1.2 1,177,263 - Pf1N1B4_1038 0.30 +0.9 1,177,285 + Pf1N1B4_1038 0.32 -0.3 1,177,286 - Pf1N1B4_1038 0.32 +3.3 1,177,286 - Pf1N1B4_1038 0.32 +1.2 1,177,286 - Pf1N1B4_1038 0.32 -0.7
Or see this region's nucleotide sequence