Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotides Pf1N1B4_101 and Pf1N1B4_102 are separated by 374 nucleotides Pf1N1B4_102 and Pf1N1B4_103 are separated by 108 nucleotides
Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082
_100
Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311
_101
Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446
_102
Pf1N1B4_103 - Histidine permease YuiF, at 108,555 to 109,871
_103
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 105.791 kb on - strand at 105.828 kb on - strand at 105.828 kb on - strand at 105.829 kb on + strand at 105.830 kb on - strand at 105.830 kb on - strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.845 kb on + strand at 105.846 kb on - strand at 105.854 kb on + strand at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.886 kb on - strand, within Pf1N1B4_100 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.227 kb on - strand, within Pf1N1B4_101 at 106.293 kb on + strand, within Pf1N1B4_101 at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.428 kb on - strand at 106.428 kb on - strand at 106.501 kb on + strand at 106.502 kb on - strand at 106.549 kb on + strand at 106.550 kb on - strand at 106.677 kb on + strand at 107.716 kb on - strand, within Pf1N1B4_102 at 107.921 kb on - strand, within Pf1N1B4_102 at 107.974 kb on + strand, within Pf1N1B4_102 at 108.091 kb on + strand, within Pf1N1B4_102 at 108.196 kb on + strand, within Pf1N1B4_102 at 108.408 kb on + strand at 108.409 kb on - strand at 108.409 kb on - strand at 108.409 kb on - strand at 108.495 kb on + strand at 108.495 kb on + strand at 108.556 kb on + strand at 108.557 kb on - strand at 108.557 kb on - strand at 108.605 kb on - strand at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand at 109.219 kb on + strand, within Pf1N1B4_103 at 109.324 kb on - strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.400 kb on - strand, within Pf1N1B4_103
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 105,791 - -0.4 105,828 - +0.2 105,828 - +1.1 105,829 + +0.3 105,830 - +0.6 105,830 - +0.8 105,833 + +1.8 105,833 + +0.8 105,833 + -0.4 105,834 - +0.4 105,834 - -0.8 105,834 - -1.6 105,834 - -0.5 105,834 - +0.5 105,834 - +1.0 105,834 - +1.6 105,834 - +0.3 105,845 + -0.4 105,846 - -0.1 105,854 + -1.9 105,885 + Pf1N1B4_100 0.13 -0.2 105,885 + Pf1N1B4_100 0.13 -0.6 105,885 + Pf1N1B4_100 0.13 +0.3 105,886 - Pf1N1B4_100 0.14 -1.2 106,226 + Pf1N1B4_101 0.48 -1.2 106,226 + Pf1N1B4_101 0.48 +2.8 106,227 - Pf1N1B4_101 0.48 -0.5 106,293 + Pf1N1B4_101 0.88 +1.0 106,424 + -1.2 106,424 + -0.2 106,424 + -2.1 106,424 + +1.2 106,428 - +0.1 106,428 - -0.8 106,501 + -0.8 106,502 - -0.2 106,549 + +0.2 106,550 - +0.8 106,677 + +0.9 107,716 - Pf1N1B4_102 0.58 +1.8 107,921 - Pf1N1B4_102 0.70 -3.6 107,974 + Pf1N1B4_102 0.73 +1.5 108,091 + Pf1N1B4_102 0.80 -0.5 108,196 + Pf1N1B4_102 0.86 +2.2 108,408 + +0.1 108,409 - -0.2 108,409 - -2.1 108,409 - +1.0 108,495 + +0.0 108,495 + +0.8 108,556 + +1.8 108,557 - +0.5 108,557 - +0.0 108,605 - -1.0 109,175 - Pf1N1B4_103 0.47 +1.0 109,175 - Pf1N1B4_103 0.47 -1.8 109,175 - Pf1N1B4_103 0.47 -1.2 109,175 - Pf1N1B4_103 0.47 +0.2 109,175 - Pf1N1B4_103 0.47 -1.2 109,175 - Pf1N1B4_103 0.47 +0.5 109,175 - Pf1N1B4_103 0.47 +1.2 109,175 - Pf1N1B4_103 0.47 +1.3 109,175 - Pf1N1B4_103 0.47 +0.8 109,175 - Pf1N1B4_103 0.47 +0.2 109,175 - +0.8 109,219 + Pf1N1B4_103 0.50 +0.4 109,324 - Pf1N1B4_103 0.58 -1.2 109,399 + Pf1N1B4_103 0.64 -1.1 109,399 + Pf1N1B4_103 0.64 +0.8 109,399 + Pf1N1B4_103 0.64 +0.4 109,400 - Pf1N1B4_103 0.64 -1.6
Or see this region's nucleotide sequence