Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1016 and Pf1N1B4_1017 are separated by 359 nucleotides Pf1N1B4_1017 and Pf1N1B4_1018 are separated by 66 nucleotides Pf1N1B4_1018 and Pf1N1B4_1019 are separated by 28 nucleotides
Pf1N1B4_1016 - FIG00954263: hypothetical protein, at 1,151,995 to 1,152,495
_1016
Pf1N1B4_1017 - FIG00957554: hypothetical protein, at 1,152,855 to 1,153,220
_1017
Pf1N1B4_1018 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 1,153,287 to 1,154,396
_1018
Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414
_1019
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1152.293 kb on + strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.407 kb on + strand, within Pf1N1B4_1016 at 1152.408 kb on - strand, within Pf1N1B4_1016 at 1152.746 kb on + strand at 1152.746 kb on + strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.751 kb on + strand at 1152.751 kb on + strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.785 kb on + strand at 1152.800 kb on - strand at 1152.800 kb on - strand at 1152.804 kb on - strand at 1152.812 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.873 kb on + strand at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand at 1153.026 kb on + strand at 1153.027 kb on - strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.219 kb on - strand at 1153.219 kb on - strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.239 kb on - strand at 1153.288 kb on + strand at 1153.288 kb on + strand at 1153.289 kb on - strand at 1153.289 kb on - strand at 1153.753 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1154.173 kb on + strand, within Pf1N1B4_1018 at 1154.245 kb on + strand, within Pf1N1B4_1018 at 1154.389 kb on + strand at 1154.389 kb on + strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.432 kb on + strand at 1154.433 kb on - strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.504 kb on + strand at 1154.577 kb on - strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.880 kb on - strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.311 kb on - strand, within Pf1N1B4_1019
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,152,293 + Pf1N1B4_1016 0.59 +0.2 1,152,294 - Pf1N1B4_1016 0.60 -1.3 1,152,294 - Pf1N1B4_1016 0.60 -0.5 1,152,294 - Pf1N1B4_1016 0.60 +0.1 1,152,294 - Pf1N1B4_1016 0.60 -0.1 1,152,294 - Pf1N1B4_1016 0.60 -0.1 1,152,294 - Pf1N1B4_1016 0.60 +1.0 1,152,407 + Pf1N1B4_1016 0.82 +0.6 1,152,408 - Pf1N1B4_1016 0.82 +0.1 1,152,746 + -0.5 1,152,746 + +0.1 1,152,747 - -0.5 1,152,747 - -0.1 1,152,747 - -2.2 1,152,747 - -1.2 1,152,751 + +1.1 1,152,751 + -0.5 1,152,752 - -0.0 1,152,752 - +1.5 1,152,752 - +1.1 1,152,752 - -0.1 1,152,752 - +0.8 1,152,785 + +1.9 1,152,800 - +0.1 1,152,800 - -0.3 1,152,804 - -0.3 1,152,812 + +0.4 1,152,829 + -0.9 1,152,829 + -0.2 1,152,829 + -0.5 1,152,829 + +0.6 1,152,829 + -0.2 1,152,829 + +2.7 1,152,829 + +0.3 1,152,830 - -0.6 1,152,830 - +0.7 1,152,830 - +0.9 1,152,830 - +0.5 1,152,830 - -1.5 1,152,871 + +0.1 1,152,871 + +0.6 1,152,871 + -1.7 1,152,872 - -0.9 1,152,872 - -2.3 1,152,872 - -0.3 1,152,872 - -1.8 1,152,872 - -0.3 1,152,873 + +0.3 1,153,026 + Pf1N1B4_1017 0.47 +1.5 1,153,026 + Pf1N1B4_1017 0.47 +1.1 1,153,026 + Pf1N1B4_1017 0.47 -0.7 1,153,026 + Pf1N1B4_1017 0.47 -0.5 1,153,026 + Pf1N1B4_1017 0.47 -1.0 1,153,026 + Pf1N1B4_1017 0.47 -0.5 1,153,026 + -0.2 1,153,026 + +1.1 1,153,027 - Pf1N1B4_1017 0.47 +0.4 1,153,048 + Pf1N1B4_1017 0.53 +1.5 1,153,048 + Pf1N1B4_1017 0.53 +0.1 1,153,048 + Pf1N1B4_1017 0.53 -0.9 1,153,048 + +0.1 1,153,048 + Pf1N1B4_1017 0.53 -1.0 1,153,049 - Pf1N1B4_1017 0.53 +0.8 1,153,049 - Pf1N1B4_1017 0.53 -2.5 1,153,049 - Pf1N1B4_1017 0.53 +0.1 1,153,150 + Pf1N1B4_1017 0.81 +0.3 1,153,150 + Pf1N1B4_1017 0.81 -0.5 1,153,150 + Pf1N1B4_1017 0.81 -0.5 1,153,150 + -0.5 1,153,218 + +0.8 1,153,218 + -1.1 1,153,218 + -1.0 1,153,218 + +0.3 1,153,218 + -0.7 1,153,219 - -1.7 1,153,219 - +0.4 1,153,238 + -0.4 1,153,238 + +0.3 1,153,238 + -1.2 1,153,239 - -0.9 1,153,288 + +0.1 1,153,288 + -0.9 1,153,289 - -1.2 1,153,289 - +1.1 1,153,753 - Pf1N1B4_1018 0.42 -0.5 1,153,900 - Pf1N1B4_1018 0.55 +0.1 1,153,900 - Pf1N1B4_1018 0.55 +0.5 1,153,900 - Pf1N1B4_1018 0.55 -0.7 1,153,900 - Pf1N1B4_1018 0.55 -2.0 1,154,173 + Pf1N1B4_1018 0.80 -1.6 1,154,245 + Pf1N1B4_1018 0.86 -0.6 1,154,389 + +0.4 1,154,389 + -0.4 1,154,390 - -0.2 1,154,390 - -0.3 1,154,390 - -1.2 1,154,432 + -0.4 1,154,433 - -1.4 1,154,486 + -0.7 1,154,486 + +1.4 1,154,486 + -0.7 1,154,486 + -0.2 1,154,486 + -0.3 1,154,486 + -0.4 1,154,486 + +0.0 1,154,486 + +2.1 1,154,486 + -0.9 1,154,486 + +0.9 1,154,486 + +0.0 1,154,486 + +1.1 1,154,487 - -0.7 1,154,487 - -0.3 1,154,487 - +0.9 1,154,487 - +1.7 1,154,487 - +0.5 1,154,504 + +0.1 1,154,577 - Pf1N1B4_1019 0.15 +2.1 1,154,793 + Pf1N1B4_1019 0.37 +0.1 1,154,793 + Pf1N1B4_1019 0.37 +0.1 1,154,793 + Pf1N1B4_1019 0.37 +0.6 1,154,794 - Pf1N1B4_1019 0.37 -1.2 1,154,794 - Pf1N1B4_1019 0.37 -1.1 1,154,794 - Pf1N1B4_1019 0.37 -0.3 1,154,880 - Pf1N1B4_1019 0.46 +1.0 1,154,902 + Pf1N1B4_1019 0.48 +0.4 1,154,902 + Pf1N1B4_1019 0.48 -0.4 1,154,903 - Pf1N1B4_1019 0.48 +1.1 1,154,903 - Pf1N1B4_1019 0.48 +0.7 1,155,146 + Pf1N1B4_1019 0.73 -0.9 1,155,146 + Pf1N1B4_1019 0.73 +0.5 1,155,146 + Pf1N1B4_1019 0.73 -0.2 1,155,146 + Pf1N1B4_1019 0.73 -0.2 1,155,147 - Pf1N1B4_1019 0.73 +0.7 1,155,147 - Pf1N1B4_1019 0.73 +0.1 1,155,147 - +0.1 1,155,147 - Pf1N1B4_1019 0.73 -0.7 1,155,311 - Pf1N1B4_1019 0.89 -1.4
Or see this region's nucleotide sequence