Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05575 and MMP_RS05580 are separated by 3 nucleotides MMP_RS05580 and MMP_RS08890 are separated by 173 nucleotides MMP_RS08890 and MMP_RS05590 are separated by 713 nucleotides
MMP_RS05575: MMP_RS05575 - hypothetical protein, at 1,068,603 to 1,069,547
_RS05575
MMP_RS05580: MMP_RS05580 - glycosyltransferase, at 1,069,551 to 1,070,360
_RS05580
MMP_RS08890: MMP_RS08890 - glycosyltransferase family 4 protein, at 1,070,534 to 1,071,628
_RS08890
MMP_RS05590: MMP_RS05590 - N-acetyl sugar amidotransferase, at 1,072,342 to 1,073,454
_RS05590
Position (kb)
1070
1071
1072 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1069.546 kb on + strand at 1069.550 kb on - strand at 1069.550 kb on - strand at 1069.592 kb on - strand at 1069.666 kb on - strand, within MMP_RS05580 at 1069.683 kb on + strand, within MMP_RS05580 at 1069.704 kb on + strand, within MMP_RS05580 at 1069.705 kb on - strand, within MMP_RS05580 at 1069.713 kb on - strand, within MMP_RS05580 at 1069.739 kb on - strand, within MMP_RS05580 at 1069.742 kb on + strand, within MMP_RS05580 at 1069.763 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.781 kb on - strand, within MMP_RS05580 at 1069.781 kb on - strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.792 kb on - strand, within MMP_RS05580 at 1069.793 kb on + strand, within MMP_RS05580 at 1069.849 kb on - strand, within MMP_RS05580 at 1069.865 kb on + strand, within MMP_RS05580 at 1069.868 kb on - strand, within MMP_RS05580 at 1069.868 kb on - strand, within MMP_RS05580 at 1069.870 kb on - strand, within MMP_RS05580 at 1069.882 kb on + strand, within MMP_RS05580 at 1069.929 kb on - strand, within MMP_RS05580 at 1069.982 kb on - strand, within MMP_RS05580 at 1069.983 kb on + strand, within MMP_RS05580 at 1069.989 kb on + strand, within MMP_RS05580 at 1069.992 kb on + strand, within MMP_RS05580 at 1070.079 kb on - strand, within MMP_RS05580 at 1070.079 kb on - strand, within MMP_RS05580 at 1070.080 kb on + strand, within MMP_RS05580 at 1070.080 kb on + strand, within MMP_RS05580 at 1070.112 kb on + strand, within MMP_RS05580 at 1070.132 kb on - strand, within MMP_RS05580 at 1070.133 kb on + strand, within MMP_RS05580 at 1070.133 kb on + strand, within MMP_RS05580 at 1070.216 kb on + strand, within MMP_RS05580 at 1070.219 kb on - strand, within MMP_RS05580 at 1070.223 kb on + strand, within MMP_RS05580 at 1070.332 kb on - strand at 1070.333 kb on + strand at 1070.333 kb on + strand at 1070.335 kb on - strand at 1070.346 kb on - strand at 1070.347 kb on + strand at 1070.348 kb on - strand at 1070.348 kb on - strand at 1070.348 kb on - strand at 1070.359 kb on + strand at 1070.370 kb on - strand at 1070.373 kb on - strand at 1070.429 kb on - strand at 1070.438 kb on + strand at 1070.466 kb on - strand at 1070.470 kb on + strand at 1070.478 kb on - strand at 1070.479 kb on + strand at 1070.479 kb on + strand at 1070.548 kb on + strand at 1070.548 kb on + strand at 1070.561 kb on + strand at 1070.614 kb on - strand at 1070.629 kb on - strand at 1070.629 kb on - strand at 1070.632 kb on - strand at 1070.632 kb on - strand at 1070.633 kb on + strand at 1070.634 kb on - strand at 1070.634 kb on - strand at 1070.688 kb on - strand, within MMP_RS08890 at 1070.688 kb on - strand, within MMP_RS08890 at 1070.688 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.694 kb on - strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.695 kb on + strand, within MMP_RS08890 at 1070.698 kb on - strand, within MMP_RS08890 at 1070.699 kb on + strand, within MMP_RS08890 at 1070.699 kb on + strand, within MMP_RS08890 at 1070.699 kb on + strand, within MMP_RS08890 at 1070.699 kb on + strand, within MMP_RS08890 at 1070.734 kb on - strand, within MMP_RS08890 at 1070.740 kb on - strand, within MMP_RS08890 at 1070.741 kb on + strand, within MMP_RS08890 at 1070.741 kb on + strand, within MMP_RS08890 at 1070.741 kb on + strand, within MMP_RS08890 at 1070.899 kb on + strand, within MMP_RS08890 at 1070.941 kb on + strand, within MMP_RS08890 at 1070.991 kb on - strand, within MMP_RS08890 at 1070.992 kb on + strand, within MMP_RS08890 at 1070.995 kb on - strand, within MMP_RS08890 at 1071.056 kb on + strand, within MMP_RS08890 at 1071.183 kb on - strand, within MMP_RS08890 at 1071.187 kb on - strand, within MMP_RS08890 at 1071.187 kb on - strand, within MMP_RS08890 at 1071.187 kb on - strand, within MMP_RS08890 at 1071.188 kb on + strand, within MMP_RS08890 at 1071.188 kb on + strand, within MMP_RS08890 at 1071.189 kb on - strand, within MMP_RS08890 at 1071.189 kb on - strand, within MMP_RS08890 at 1071.207 kb on - strand, within MMP_RS08890 at 1071.208 kb on + strand, within MMP_RS08890 at 1071.231 kb on + strand, within MMP_RS08890 at 1071.231 kb on + strand, within MMP_RS08890 at 1071.239 kb on + strand, within MMP_RS08890 at 1071.244 kb on + strand, within MMP_RS08890 at 1071.244 kb on + strand, within MMP_RS08890 at 1071.244 kb on + strand, within MMP_RS08890 at 1071.301 kb on - strand, within MMP_RS08890 at 1071.592 kb on - strand at 1071.599 kb on - strand at 1071.617 kb on + strand at 1071.617 kb on + strand at 1071.622 kb on - strand at 1071.626 kb on - strand at 1071.702 kb on - strand at 1071.755 kb on + strand at 1071.755 kb on + strand at 1071.768 kb on - strand at 1071.769 kb on + strand at 1071.769 kb on + strand at 1071.823 kb on - strand at 1071.824 kb on + strand at 1071.837 kb on - strand at 1071.838 kb on + strand at 1071.848 kb on - strand at 1071.848 kb on - strand at 1071.848 kb on - strand at 1071.849 kb on + strand at 1071.849 kb on + strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.854 kb on + strand at 1071.866 kb on + strand at 1071.926 kb on - strand at 1072.001 kb on + strand at 1072.002 kb on - strand at 1072.007 kb on - strand at 1072.041 kb on + strand at 1072.045 kb on - strand at 1072.063 kb on - strand at 1072.063 kb on - strand at 1072.070 kb on + strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.105 kb on + strand at 1072.114 kb on - strand at 1072.114 kb on - strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.178 kb on - strand at 1072.184 kb on + strand at 1072.284 kb on - strand at 1072.285 kb on + strand at 1072.285 kb on + strand at 1072.285 kb on + strand at 1072.288 kb on - strand at 1072.288 kb on - strand at 1072.288 kb on - strand at 1072.289 kb on + strand at 1072.289 kb on + strand at 1072.300 kb on - strand at 1072.301 kb on + strand at 1072.304 kb on - strand at 1072.304 kb on - strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.310 kb on + strand at 1072.310 kb on + strand at 1072.331 kb on + strand at 1072.334 kb on - strand at 1072.337 kb on - strand at 1072.359 kb on - strand at 1072.371 kb on - strand at 1072.429 kb on - strand at 1072.429 kb on - strand at 1072.430 kb on + strand at 1072.464 kb on + strand, within MMP_RS05590 at 1072.464 kb on + strand, within MMP_RS05590 at 1072.537 kb on - strand, within MMP_RS05590 at 1072.576 kb on + strand, within MMP_RS05590 at 1072.579 kb on - strand, within MMP_RS05590 at 1072.579 kb on - strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.583 kb on - strand, within MMP_RS05590 at 1072.583 kb on - strand, within MMP_RS05590 at 1072.588 kb on - strand, within MMP_RS05590 at 1072.588 kb on - strand, within MMP_RS05590 at 1072.589 kb on + strand, within MMP_RS05590 at 1072.589 kb on + strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.612 kb on + strand, within MMP_RS05590
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,069,546 + +2.4 1,069,550 - +1.9 1,069,550 - +2.4 1,069,592 - +2.9 1,069,666 - MMP_RS05580 0.14 +1.4 1,069,683 + MMP_RS05580 0.16 +3.8 1,069,704 + MMP_RS05580 0.19 +2.8 1,069,705 - MMP_RS05580 0.19 +2.3 1,069,713 - MMP_RS05580 0.20 +2.0 1,069,739 - MMP_RS05580 0.23 +2.8 1,069,742 + MMP_RS05580 0.24 +2.1 1,069,763 + MMP_RS05580 0.26 +2.7 1,069,780 + MMP_RS05580 0.28 +2.6 1,069,780 + MMP_RS05580 0.28 +2.1 1,069,780 + MMP_RS05580 0.28 +2.7 1,069,780 + MMP_RS05580 0.28 +1.9 1,069,781 - MMP_RS05580 0.28 +3.1 1,069,781 - MMP_RS05580 0.28 +0.7 1,069,782 + MMP_RS05580 0.29 +2.5 1,069,782 + MMP_RS05580 0.29 +2.8 1,069,782 + MMP_RS05580 0.29 +2.8 1,069,782 + MMP_RS05580 0.29 +2.7 1,069,784 - MMP_RS05580 0.29 +2.0 1,069,784 - MMP_RS05580 0.29 +4.0 1,069,784 - MMP_RS05580 0.29 +2.3 1,069,784 - MMP_RS05580 0.29 +2.4 1,069,785 + MMP_RS05580 0.29 +2.9 1,069,785 + MMP_RS05580 0.29 +2.8 1,069,785 + MMP_RS05580 0.29 +0.7 1,069,785 + MMP_RS05580 0.29 +2.6 1,069,785 + MMP_RS05580 0.29 +2.3 1,069,792 - MMP_RS05580 0.30 +2.3 1,069,793 + MMP_RS05580 0.30 +2.4 1,069,849 - MMP_RS05580 0.37 +2.5 1,069,865 + MMP_RS05580 0.39 +2.5 1,069,868 - MMP_RS05580 0.39 +2.8 1,069,868 - MMP_RS05580 0.39 +2.8 1,069,870 - MMP_RS05580 0.39 +2.9 1,069,882 + MMP_RS05580 0.41 +2.8 1,069,929 - MMP_RS05580 0.47 +2.6 1,069,982 - MMP_RS05580 0.53 +3.2 1,069,983 + MMP_RS05580 0.53 +2.6 1,069,989 + MMP_RS05580 0.54 +4.4 1,069,992 + MMP_RS05580 0.54 +2.6 1,070,079 - MMP_RS05580 0.65 +2.5 1,070,079 - MMP_RS05580 0.65 +2.2 1,070,080 + MMP_RS05580 0.65 +2.3 1,070,080 + MMP_RS05580 0.65 +2.0 1,070,112 + MMP_RS05580 0.69 +1.8 1,070,132 - MMP_RS05580 0.72 +2.3 1,070,133 + MMP_RS05580 0.72 +2.4 1,070,133 + MMP_RS05580 0.72 +1.5 1,070,216 + MMP_RS05580 0.82 +2.5 1,070,219 - MMP_RS05580 0.82 +2.1 1,070,223 + MMP_RS05580 0.83 +2.4 1,070,332 - +2.7 1,070,333 + +2.8 1,070,333 + +2.4 1,070,335 - +2.4 1,070,346 - +1.7 1,070,347 + +3.1 1,070,348 - -2.6 1,070,348 - -1.7 1,070,348 - -3.3 1,070,359 + -1.9 1,070,370 - -2.0 1,070,373 - -2.2 1,070,429 - +4.0 1,070,438 + +3.8 1,070,466 - -0.4 1,070,470 + +0.4 1,070,478 - -1.0 1,070,479 + -0.1 1,070,479 + -0.8 1,070,548 + +4.0 1,070,548 + -4.4 1,070,561 + -2.7 1,070,614 - -4.0 1,070,629 - -2.9 1,070,629 - +4.0 1,070,632 - -2.7 1,070,632 - -1.5 1,070,633 + -1.2 1,070,634 - -1.8 1,070,634 - -2.8 1,070,688 - MMP_RS08890 0.14 -2.2 1,070,688 - MMP_RS08890 0.14 -2.8 1,070,688 - MMP_RS08890 0.14 -1.0 1,070,694 - MMP_RS08890 0.15 -1.2 1,070,694 - MMP_RS08890 0.15 -1.4 1,070,694 - MMP_RS08890 0.15 -2.0 1,070,694 - MMP_RS08890 0.15 -2.1 1,070,694 - MMP_RS08890 0.15 -3.0 1,070,694 - MMP_RS08890 0.15 -4.5 1,070,694 - MMP_RS08890 0.15 -1.7 1,070,695 + MMP_RS08890 0.15 -2.3 1,070,695 + MMP_RS08890 0.15 -2.8 1,070,695 + MMP_RS08890 0.15 -2.1 1,070,695 + MMP_RS08890 0.15 -3.0 1,070,695 + MMP_RS08890 0.15 -3.8 1,070,695 + MMP_RS08890 0.15 -2.1 1,070,695 + MMP_RS08890 0.15 -1.0 1,070,698 - MMP_RS08890 0.15 -3.7 1,070,699 + MMP_RS08890 0.15 -1.5 1,070,699 + MMP_RS08890 0.15 -1.2 1,070,699 + MMP_RS08890 0.15 -3.2 1,070,699 + MMP_RS08890 0.15 -2.8 1,070,734 - MMP_RS08890 0.18 -2.7 1,070,740 - MMP_RS08890 0.19 -4.2 1,070,741 + MMP_RS08890 0.19 -4.6 1,070,741 + MMP_RS08890 0.19 -1.6 1,070,741 + MMP_RS08890 0.19 -3.7 1,070,899 + MMP_RS08890 0.33 -1.9 1,070,941 + MMP_RS08890 0.37 -2.3 1,070,991 - MMP_RS08890 0.42 -3.5 1,070,992 + MMP_RS08890 0.42 -1.9 1,070,995 - MMP_RS08890 0.42 -1.9 1,071,056 + MMP_RS08890 0.48 -2.1 1,071,183 - MMP_RS08890 0.59 -3.0 1,071,187 - MMP_RS08890 0.60 -2.2 1,071,187 - MMP_RS08890 0.60 -2.9 1,071,187 - MMP_RS08890 0.60 -3.7 1,071,188 + MMP_RS08890 0.60 -2.0 1,071,188 + MMP_RS08890 0.60 +4.1 1,071,189 - MMP_RS08890 0.60 -1.9 1,071,189 - MMP_RS08890 0.60 -3.9 1,071,207 - MMP_RS08890 0.61 -2.5 1,071,208 + MMP_RS08890 0.62 -1.0 1,071,231 + MMP_RS08890 0.64 +3.7 1,071,231 + MMP_RS08890 0.64 -1.7 1,071,239 + MMP_RS08890 0.64 -2.0 1,071,244 + MMP_RS08890 0.65 -2.4 1,071,244 + MMP_RS08890 0.65 -3.9 1,071,244 + MMP_RS08890 0.65 -1.9 1,071,301 - MMP_RS08890 0.70 -3.8 1,071,592 - -1.6 1,071,599 - -2.3 1,071,617 + -0.3 1,071,617 + +0.5 1,071,622 - +0.5 1,071,626 - -1.0 1,071,702 - +0.2 1,071,755 + -0.1 1,071,755 + +0.2 1,071,768 - -1.8 1,071,769 + +0.1 1,071,769 + +0.3 1,071,823 - -0.8 1,071,824 + +0.1 1,071,837 - -0.1 1,071,838 + -0.0 1,071,848 - +1.1 1,071,848 - -0.3 1,071,848 - +0.5 1,071,849 + -0.3 1,071,849 + -0.2 1,071,851 - +0.0 1,071,851 - -0.9 1,071,851 - -0.3 1,071,851 - +0.1 1,071,853 - +0.2 1,071,853 - -0.3 1,071,853 - +0.2 1,071,853 - -1.0 1,071,854 + -1.0 1,071,866 + +0.2 1,071,926 - +0.4 1,072,001 + +0.6 1,072,002 - +0.8 1,072,007 - -0.3 1,072,041 + -0.4 1,072,045 - +0.4 1,072,063 - -0.2 1,072,063 - -0.1 1,072,070 + +0.3 1,072,104 - +0.6 1,072,104 - +0.4 1,072,104 - +5.2 1,072,105 + -0.8 1,072,114 - -0.5 1,072,114 - -1.7 1,072,115 + -0.6 1,072,115 + +0.7 1,072,115 + -0.4 1,072,115 + -1.0 1,072,178 - -0.8 1,072,184 + -0.3 1,072,284 - +0.6 1,072,285 + +0.5 1,072,285 + -0.9 1,072,285 + +0.3 1,072,288 - +3.5 1,072,288 - -1.1 1,072,288 - -0.5 1,072,289 + +4.5 1,072,289 + +0.7 1,072,300 - -0.5 1,072,301 + +0.3 1,072,304 - -0.4 1,072,304 - -0.8 1,072,305 + -0.3 1,072,305 + -0.3 1,072,305 + -1.0 1,072,305 + -0.4 1,072,305 + +4.4 1,072,310 + +0.6 1,072,310 + -0.4 1,072,331 + +0.3 1,072,334 - -0.4 1,072,337 - -0.3 1,072,359 - +0.4 1,072,371 - +0.3 1,072,429 - -0.5 1,072,429 - -2.0 1,072,430 + -0.4 1,072,464 + MMP_RS05590 0.11 +0.7 1,072,464 + MMP_RS05590 0.11 +0.2 1,072,537 - MMP_RS05590 0.18 +0.1 1,072,576 + MMP_RS05590 0.21 +0.5 1,072,579 - MMP_RS05590 0.21 -0.2 1,072,579 - MMP_RS05590 0.21 +4.2 1,072,580 + MMP_RS05590 0.21 +0.1 1,072,580 + MMP_RS05590 0.21 -1.4 1,072,580 + MMP_RS05590 0.21 -0.5 1,072,580 + MMP_RS05590 0.21 +0.2 1,072,580 + MMP_RS05590 0.21 +0.7 1,072,580 + MMP_RS05590 0.21 -0.1 1,072,580 + MMP_RS05590 0.21 -0.9 1,072,580 + MMP_RS05590 0.21 -1.0 1,072,583 - MMP_RS05590 0.22 -1.3 1,072,583 - MMP_RS05590 0.22 -1.0 1,072,588 - MMP_RS05590 0.22 -0.0 1,072,588 - MMP_RS05590 0.22 -1.3 1,072,589 + MMP_RS05590 0.22 +0.2 1,072,589 + MMP_RS05590 0.22 -1.3 1,072,592 - MMP_RS05590 0.22 +0.8 1,072,592 - MMP_RS05590 0.22 -0.7 1,072,592 - MMP_RS05590 0.22 +4.6 1,072,592 - MMP_RS05590 0.22 -0.6 1,072,593 + MMP_RS05590 0.23 +0.4 1,072,593 + MMP_RS05590 0.23 +4.2 1,072,593 + MMP_RS05590 0.23 -0.3 1,072,612 + MMP_RS05590 0.24 -1.0
Or see this region's nucleotide sequence