Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07690 and MMP_RS07695 are separated by 24 nucleotides MMP_RS07695 and MMP_RS07700 overlap by 4 nucleotides MMP_RS07700 and MMP_RS07705 are separated by 17 nucleotides
MMP_RS07690: MMP_RS07690 - phenylalanine--tRNA ligase subunit alpha, at 1,457,002 to 1,458,504
_RS07690
MMP_RS07695: MMP_RS07695 - DUF3236 domain-containing protein, at 1,458,529 to 1,459,008
_RS07695
MMP_RS07700: MMP_RS07700 - DUF1188 domain-containing protein, at 1,459,005 to 1,459,787
_RS07700
MMP_RS07705: MMP_RS07705 - transcriptional regulator, at 1,459,805 to 1,460,317
_RS07705
Position (kb) 1458
1459
1460 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1458.101 kb on - strand at 1458.439 kb on + strand at 1458.630 kb on + strand, within MMP_RS07695 at 1458.654 kb on + strand, within MMP_RS07695 at 1458.683 kb on - strand, within MMP_RS07695 at 1458.789 kb on + strand, within MMP_RS07695 at 1458.818 kb on - strand, within MMP_RS07695 at 1458.824 kb on - strand, within MMP_RS07695 at 1458.941 kb on - strand, within MMP_RS07695 at 1458.942 kb on + strand, within MMP_RS07695 at 1459.019 kb on - strand at 1459.108 kb on + strand, within MMP_RS07700 at 1459.115 kb on + strand, within MMP_RS07700 at 1459.131 kb on + strand, within MMP_RS07700 at 1459.144 kb on - strand, within MMP_RS07700 at 1459.170 kb on - strand, within MMP_RS07700 at 1459.208 kb on - strand, within MMP_RS07700 at 1459.234 kb on - strand, within MMP_RS07700 at 1459.235 kb on + strand, within MMP_RS07700 at 1459.297 kb on - strand, within MMP_RS07700 at 1459.347 kb on + strand, within MMP_RS07700 at 1459.370 kb on + strand, within MMP_RS07700 at 1459.449 kb on + strand, within MMP_RS07700 at 1459.452 kb on + strand, within MMP_RS07700 at 1459.511 kb on - strand, within MMP_RS07700 at 1459.555 kb on - strand, within MMP_RS07700 at 1459.556 kb on + strand, within MMP_RS07700 at 1459.556 kb on + strand, within MMP_RS07700 at 1459.589 kb on + strand, within MMP_RS07700 at 1459.699 kb on - strand, within MMP_RS07700 at 1459.722 kb on - strand at 1459.722 kb on - strand at 1459.763 kb on + strand at 1459.763 kb on + strand at 1459.841 kb on + strand at 1459.861 kb on - strand, within MMP_RS07705 at 1459.907 kb on - strand, within MMP_RS07705 at 1459.908 kb on + strand, within MMP_RS07705 at 1459.954 kb on + strand, within MMP_RS07705 at 1459.956 kb on + strand, within MMP_RS07705 at 1459.984 kb on - strand, within MMP_RS07705 at 1459.985 kb on + strand, within MMP_RS07705
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,458,101 - -2.0 1,458,439 + -2.3 1,458,630 + MMP_RS07695 0.21 -0.4 1,458,654 + MMP_RS07695 0.26 -1.9 1,458,683 - MMP_RS07695 0.32 -0.9 1,458,789 + MMP_RS07695 0.54 -1.3 1,458,818 - MMP_RS07695 0.60 -1.6 1,458,824 - MMP_RS07695 0.61 -1.1 1,458,941 - MMP_RS07695 0.86 -1.3 1,458,942 + MMP_RS07695 0.86 -0.9 1,459,019 - -0.6 1,459,108 + MMP_RS07700 0.13 -1.2 1,459,115 + MMP_RS07700 0.14 -0.7 1,459,131 + MMP_RS07700 0.16 -0.9 1,459,144 - MMP_RS07700 0.18 -1.2 1,459,170 - MMP_RS07700 0.21 -0.3 1,459,208 - MMP_RS07700 0.26 -1.9 1,459,234 - MMP_RS07700 0.29 +0.2 1,459,235 + MMP_RS07700 0.29 -1.3 1,459,297 - MMP_RS07700 0.37 -0.6 1,459,347 + MMP_RS07700 0.44 -1.0 1,459,370 + MMP_RS07700 0.47 -2.4 1,459,449 + MMP_RS07700 0.57 +0.4 1,459,452 + MMP_RS07700 0.57 -0.1 1,459,511 - MMP_RS07700 0.65 -1.6 1,459,555 - MMP_RS07700 0.70 +0.4 1,459,556 + MMP_RS07700 0.70 -1.5 1,459,556 + MMP_RS07700 0.70 -0.7 1,459,589 + MMP_RS07700 0.75 -1.0 1,459,699 - MMP_RS07700 0.89 -0.8 1,459,722 - -0.2 1,459,722 - -1.5 1,459,763 + -0.3 1,459,763 + +0.1 1,459,841 + +0.0 1,459,861 - MMP_RS07705 0.11 +0.4 1,459,907 - MMP_RS07705 0.20 +0.1 1,459,908 + MMP_RS07705 0.20 +0.5 1,459,954 + MMP_RS07705 0.29 +4.7 1,459,956 + MMP_RS07705 0.29 +4.5 1,459,984 - MMP_RS07705 0.35 +0.4 1,459,985 + MMP_RS07705 0.35 -0.2
Or see this region's nucleotide sequence