Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04470 and MMP_RS04475 overlap by 35 nucleotides MMP_RS04475 and MMP_RS04480 are separated by 575 nucleotides MMP_RS04480 and MMP_RS04485 are separated by 0 nucleotides MMP_RS04485 and MMP_RS04490 are separated by 149 nucleotides
MMP_RS04470: MMP_RS04470 - nitrogenase, at 849,999 to 851,375
_RS04470
MMP_RS04475: MMP_RS04475 - nitrogen fixation, at 851,341 to 851,661
_RS04475
MMP_RS04480: MMP_RS04480 - lysine 2 3-aminomutase, at 852,237 to 853,538
_RS04480
MMP_RS04485: MMP_RS04485 - putative beta-lysine N-acetyltransferase, at 853,539 to 854,363
_RS04485
MMP_RS04490: MMP_RS04490 - CBS domain-containing protein, at 854,513 to 854,989
_RS04490
Position (kb) 852
853
854 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 851.239 kb on + strand at 851.240 kb on - strand at 851.241 kb on + strand at 851.260 kb on - strand at 851.261 kb on + strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.267 kb on - strand at 851.268 kb on + strand at 851.268 kb on + strand at 851.269 kb on - strand at 851.269 kb on - strand at 851.276 kb on - strand at 851.293 kb on + strand at 851.382 kb on + strand, within MMP_RS04475 at 851.382 kb on + strand, within MMP_RS04475 at 851.444 kb on - strand, within MMP_RS04475 at 851.484 kb on - strand, within MMP_RS04475 at 851.486 kb on - strand, within MMP_RS04475 at 851.513 kb on + strand, within MMP_RS04475 at 851.934 kb on - strand at 851.944 kb on - strand at 851.945 kb on + strand at 851.957 kb on + strand at 851.966 kb on - strand at 851.971 kb on - strand at 851.972 kb on + strand at 851.975 kb on - strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.998 kb on - strand at 852.002 kb on - strand at 852.041 kb on - strand at 852.046 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.054 kb on + strand at 852.056 kb on - strand at 852.057 kb on + strand at 852.057 kb on + strand at 852.074 kb on + strand at 852.102 kb on - strand at 852.102 kb on - strand at 852.103 kb on + strand at 852.105 kb on + strand at 852.179 kb on - strand at 852.180 kb on + strand at 852.247 kb on - strand at 852.248 kb on + strand at 852.260 kb on - strand at 852.265 kb on - strand at 852.319 kb on + strand at 852.421 kb on - strand, within MMP_RS04480 at 852.422 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.443 kb on - strand, within MMP_RS04480 at 852.451 kb on + strand, within MMP_RS04480 at 852.453 kb on - strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.463 kb on - strand, within MMP_RS04480 at 852.500 kb on - strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.622 kb on - strand, within MMP_RS04480 at 852.627 kb on - strand, within MMP_RS04480 at 852.632 kb on + strand, within MMP_RS04480 at 852.741 kb on + strand, within MMP_RS04480 at 852.752 kb on - strand, within MMP_RS04480 at 852.753 kb on + strand, within MMP_RS04480 at 852.757 kb on - strand, within MMP_RS04480 at 852.812 kb on + strand, within MMP_RS04480 at 852.840 kb on + strand, within MMP_RS04480 at 852.858 kb on + strand, within MMP_RS04480 at 852.953 kb on - strand, within MMP_RS04480 at 852.953 kb on - strand, within MMP_RS04480 at 852.954 kb on + strand, within MMP_RS04480 at 853.077 kb on - strand, within MMP_RS04480 at 853.077 kb on - strand, within MMP_RS04480 at 853.078 kb on + strand, within MMP_RS04480 at 853.129 kb on + strand, within MMP_RS04480 at 853.131 kb on - strand, within MMP_RS04480 at 853.131 kb on - strand, within MMP_RS04480 at 853.132 kb on + strand, within MMP_RS04480 at 853.214 kb on + strand, within MMP_RS04480 at 853.245 kb on + strand, within MMP_RS04480 at 853.248 kb on - strand, within MMP_RS04480 at 853.248 kb on - strand, within MMP_RS04480 at 853.249 kb on + strand, within MMP_RS04480 at 853.255 kb on - strand, within MMP_RS04480 at 853.299 kb on - strand, within MMP_RS04480 at 853.300 kb on + strand, within MMP_RS04480 at 853.362 kb on - strand, within MMP_RS04480 at 853.478 kb on + strand at 853.495 kb on - strand at 853.495 kb on - strand at 853.496 kb on + strand at 853.580 kb on + strand at 853.600 kb on - strand at 853.601 kb on + strand at 853.601 kb on + strand at 853.601 kb on + strand at 853.602 kb on - strand at 853.602 kb on - strand at 853.603 kb on + strand at 853.604 kb on - strand at 853.605 kb on + strand at 853.735 kb on + strand, within MMP_RS04485 at 853.782 kb on - strand, within MMP_RS04485 at 853.783 kb on + strand, within MMP_RS04485 at 853.787 kb on - strand, within MMP_RS04485 at 853.788 kb on + strand, within MMP_RS04485 at 853.788 kb on + strand, within MMP_RS04485 at 853.945 kb on - strand, within MMP_RS04485 at 853.945 kb on - strand, within MMP_RS04485 at 854.007 kb on + strand, within MMP_RS04485 at 854.007 kb on + strand, within MMP_RS04485 at 854.008 kb on - strand, within MMP_RS04485 at 854.009 kb on + strand, within MMP_RS04485 at 854.011 kb on - strand, within MMP_RS04485 at 854.032 kb on - strand, within MMP_RS04485 at 854.097 kb on + strand, within MMP_RS04485 at 854.097 kb on + strand, within MMP_RS04485 at 854.167 kb on + strand, within MMP_RS04485 at 854.175 kb on - strand, within MMP_RS04485 at 854.177 kb on - strand, within MMP_RS04485 at 854.178 kb on + strand, within MMP_RS04485 at 854.178 kb on + strand, within MMP_RS04485 at 854.178 kb on + strand, within MMP_RS04485 at 854.178 kb on + strand, within MMP_RS04485 at 854.178 kb on + strand, within MMP_RS04485 at 854.279 kb on - strand, within MMP_RS04485 at 854.303 kb on - strand at 854.306 kb on - strand at 854.306 kb on - strand at 854.346 kb on - strand at 854.425 kb on + strand at 854.428 kb on - strand at 854.428 kb on - strand at 854.428 kb on - strand at 854.428 kb on - strand at 854.429 kb on + strand at 854.429 kb on + strand at 854.429 kb on + strand at 854.429 kb on + strand at 854.429 kb on + strand at 854.429 kb on + strand at 854.430 kb on - strand at 854.431 kb on + strand at 854.431 kb on + strand at 854.481 kb on - strand at 854.486 kb on - strand at 854.492 kb on + strand at 854.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 851,239 + +0.7 851,240 - +0.2 851,241 + -0.7 851,260 - -0.2 851,261 + -0.8 851,264 - +0.2 851,264 - -0.1 851,264 - +0.2 851,267 - -0.3 851,268 + +0.0 851,268 + +0.4 851,269 - -0.3 851,269 - +0.1 851,276 - -0.0 851,293 + -0.4 851,382 + MMP_RS04475 0.13 -1.7 851,382 + MMP_RS04475 0.13 +0.1 851,444 - MMP_RS04475 0.32 -0.9 851,484 - MMP_RS04475 0.45 -0.2 851,486 - MMP_RS04475 0.45 -0.3 851,513 + MMP_RS04475 0.54 -0.3 851,934 - +0.4 851,944 - +0.1 851,945 + +0.7 851,957 + -0.0 851,966 - -0.2 851,971 - -0.7 851,972 + +0.1 851,975 - -0.3 851,976 + -2.2 851,976 + -0.9 851,976 + -0.2 851,998 - -0.4 852,002 - -0.3 852,041 - -0.7 852,046 - +0.5 852,051 - +0.1 852,051 - +0.0 852,051 - -0.2 852,051 - -0.2 852,054 + -0.6 852,056 - -0.4 852,057 + -0.8 852,057 + -1.1 852,074 + -1.4 852,102 - -3.1 852,102 - -2.7 852,103 + -2.6 852,105 + -3.1 852,179 - -1.8 852,180 + -2.0 852,247 - -1.5 852,248 + -2.2 852,260 - -3.9 852,265 - -2.8 852,319 + -1.6 852,421 - MMP_RS04480 0.14 -2.4 852,422 + MMP_RS04480 0.14 -1.0 852,428 + MMP_RS04480 0.15 -1.0 852,428 + MMP_RS04480 0.15 -3.1 852,443 - MMP_RS04480 0.16 -2.1 852,451 + MMP_RS04480 0.16 -2.2 852,453 - MMP_RS04480 0.17 -1.6 852,454 + MMP_RS04480 0.17 -1.6 852,454 + MMP_RS04480 0.17 -2.5 852,454 + MMP_RS04480 0.17 -3.6 852,463 - MMP_RS04480 0.17 -1.7 852,500 - MMP_RS04480 0.20 -3.4 852,533 + MMP_RS04480 0.23 -0.2 852,533 + MMP_RS04480 0.23 -1.9 852,622 - MMP_RS04480 0.30 -2.1 852,627 - MMP_RS04480 0.30 -1.4 852,632 + MMP_RS04480 0.30 -1.4 852,741 + MMP_RS04480 0.39 -2.2 852,752 - MMP_RS04480 0.40 -1.3 852,753 + MMP_RS04480 0.40 -3.0 852,757 - MMP_RS04480 0.40 -1.6 852,812 + MMP_RS04480 0.44 -2.9 852,840 + MMP_RS04480 0.46 -1.8 852,858 + MMP_RS04480 0.48 -2.2 852,953 - MMP_RS04480 0.55 -1.7 852,953 - MMP_RS04480 0.55 -1.2 852,954 + MMP_RS04480 0.55 -1.0 853,077 - MMP_RS04480 0.65 -0.3 853,077 - MMP_RS04480 0.65 -1.5 853,078 + MMP_RS04480 0.65 -0.5 853,129 + MMP_RS04480 0.69 -2.8 853,131 - MMP_RS04480 0.69 +3.7 853,131 - MMP_RS04480 0.69 -1.3 853,132 + MMP_RS04480 0.69 -1.2 853,214 + MMP_RS04480 0.75 -2.5 853,245 + MMP_RS04480 0.77 -2.7 853,248 - MMP_RS04480 0.78 -1.6 853,248 - MMP_RS04480 0.78 -1.1 853,249 + MMP_RS04480 0.78 -1.7 853,255 - MMP_RS04480 0.78 -1.2 853,299 - MMP_RS04480 0.82 -1.8 853,300 + MMP_RS04480 0.82 -2.1 853,362 - MMP_RS04480 0.86 +3.3 853,478 + -0.6 853,495 - -0.4 853,495 - -1.5 853,496 + -2.7 853,580 + -1.2 853,600 - -2.9 853,601 + -1.8 853,601 + -2.7 853,601 + -2.0 853,602 - -3.7 853,602 - -2.0 853,603 + -1.5 853,604 - -3.9 853,605 + -1.5 853,735 + MMP_RS04485 0.24 -1.6 853,782 - MMP_RS04485 0.29 -1.9 853,783 + MMP_RS04485 0.30 -2.2 853,787 - MMP_RS04485 0.30 -2.1 853,788 + MMP_RS04485 0.30 -2.1 853,788 + MMP_RS04485 0.30 -2.0 853,945 - MMP_RS04485 0.49 -2.3 853,945 - MMP_RS04485 0.49 -2.3 854,007 + MMP_RS04485 0.57 -2.7 854,007 + MMP_RS04485 0.57 -3.0 854,008 - MMP_RS04485 0.57 -2.3 854,009 + MMP_RS04485 0.57 -1.7 854,011 - MMP_RS04485 0.57 -4.2 854,032 - MMP_RS04485 0.60 -1.4 854,097 + MMP_RS04485 0.68 -2.2 854,097 + MMP_RS04485 0.68 -3.5 854,167 + MMP_RS04485 0.76 -2.1 854,175 - MMP_RS04485 0.77 -1.7 854,177 - MMP_RS04485 0.77 -3.3 854,178 + MMP_RS04485 0.77 -1.9 854,178 + MMP_RS04485 0.77 -3.4 854,178 + MMP_RS04485 0.77 -3.0 854,178 + MMP_RS04485 0.77 -1.8 854,178 + MMP_RS04485 0.77 -3.5 854,279 - MMP_RS04485 0.90 -2.3 854,303 - -1.9 854,306 - -3.5 854,306 - -2.3 854,346 - -1.2 854,425 + -0.3 854,428 - -0.7 854,428 - +0.2 854,428 - -0.3 854,428 - -0.6 854,429 + -0.4 854,429 + -0.0 854,429 + -0.3 854,429 + +0.2 854,429 + +0.1 854,429 + +4.1 854,430 - -0.0 854,431 + +0.3 854,431 + -0.1 854,481 - -1.0 854,486 - +4.5 854,492 + -0.6 854,492 + +4.0
Or see this region's nucleotide sequence