Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02420 and MMP_RS02425 are separated by 119 nucleotides MMP_RS02425 and MMP_RS02430 are separated by 168 nucleotides MMP_RS02430 and MMP_RS02435 are separated by 71 nucleotides MMP_RS02435 and MMP_RS02440 are separated by 345 nucleotides
MMP_RS02420: MMP_RS02420 - DEAD/DEAH box helicase, at 450,214 to 451,830
_RS02420
MMP_RS02425: MMP_RS02425 - hypothetical protein, at 451,950 to 452,144
_RS02425
MMP_RS02430: MMP_RS02430 - hypothetical protein, at 452,313 to 453,959
_RS02430
MMP_RS02435: MMP_RS02435 - tRNA-Ser, at 454,031 to 454,115
_RS02435
MMP_RS02440: MMP_RS02440 - hypothetical protein, at 454,461 to 454,838
_RS02440
Position (kb) 452
453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 451.335 kb on - strand at 451.336 kb on + strand at 451.338 kb on + strand at 451.496 kb on - strand at 451.602 kb on + strand at 451.614 kb on + strand at 451.707 kb on + strand at 451.832 kb on - strand at 451.958 kb on - strand at 451.988 kb on - strand, within MMP_RS02425 at 452.045 kb on - strand, within MMP_RS02425 at 452.049 kb on + strand, within MMP_RS02425 at 452.066 kb on - strand, within MMP_RS02425 at 452.082 kb on - strand, within MMP_RS02425 at 452.090 kb on - strand, within MMP_RS02425 at 452.107 kb on + strand, within MMP_RS02425 at 452.139 kb on - strand at 452.161 kb on - strand at 452.162 kb on + strand at 452.162 kb on + strand at 452.172 kb on - strand at 452.184 kb on - strand at 452.184 kb on - strand at 452.268 kb on + strand at 452.281 kb on - strand at 452.282 kb on + strand at 452.322 kb on - strand at 452.322 kb on - strand at 452.372 kb on - strand at 452.401 kb on - strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.477 kb on - strand at 452.478 kb on + strand, within MMP_RS02430 at 452.481 kb on - strand, within MMP_RS02430 at 452.515 kb on - strand, within MMP_RS02430 at 452.558 kb on - strand, within MMP_RS02430 at 452.560 kb on - strand, within MMP_RS02430 at 452.601 kb on + strand, within MMP_RS02430 at 452.760 kb on + strand, within MMP_RS02430 at 452.791 kb on - strand, within MMP_RS02430 at 452.792 kb on + strand, within MMP_RS02430 at 452.862 kb on - strand, within MMP_RS02430 at 453.079 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.451 kb on - strand, within MMP_RS02430 at 453.452 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.463 kb on - strand, within MMP_RS02430 at 453.633 kb on + strand, within MMP_RS02430 at 453.670 kb on - strand, within MMP_RS02430 at 453.671 kb on + strand, within MMP_RS02430 at 453.676 kb on - strand, within MMP_RS02430 at 453.677 kb on + strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.722 kb on - strand, within MMP_RS02430 at 453.734 kb on - strand, within MMP_RS02430 at 453.735 kb on + strand, within MMP_RS02430 at 453.745 kb on - strand, within MMP_RS02430 at 453.858 kb on + strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.931 kb on - strand at 453.965 kb on + strand at 453.970 kb on - strand at 453.971 kb on + strand at 454.170 kb on - strand at 454.185 kb on - strand at 454.196 kb on - strand at 454.312 kb on + strand at 454.312 kb on + strand at 454.314 kb on - strand at 454.315 kb on + strand at 454.504 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.515 kb on - strand, within MMP_RS02440 at 454.516 kb on + strand, within MMP_RS02440 at 454.573 kb on + strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.841 kb on + strand at 454.862 kb on - strand at 454.944 kb on - strand at 454.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 451,335 - -0.7 451,336 + -0.4 451,338 + -2.8 451,496 - -0.7 451,602 + +0.1 451,614 + -0.4 451,707 + -0.6 451,832 - +0.3 451,958 - +0.9 451,988 - MMP_RS02425 0.19 +1.1 452,045 - MMP_RS02425 0.49 -0.1 452,049 + MMP_RS02425 0.51 +0.0 452,066 - MMP_RS02425 0.59 +1.0 452,082 - MMP_RS02425 0.68 +0.1 452,090 - MMP_RS02425 0.72 +0.4 452,107 + MMP_RS02425 0.81 +4.4 452,139 - -0.6 452,161 - +0.6 452,162 + -0.3 452,162 + +0.6 452,172 - -0.0 452,184 - +0.5 452,184 - +0.4 452,268 + +0.1 452,281 - +0.1 452,282 + +0.3 452,322 - +0.1 452,322 - -1.2 452,372 - +0.2 452,401 - +0.5 452,469 + -0.6 452,469 + +0.8 452,469 + -0.1 452,477 - +0.6 452,478 + MMP_RS02430 0.10 +4.2 452,481 - MMP_RS02430 0.10 +0.5 452,515 - MMP_RS02430 0.12 -1.0 452,558 - MMP_RS02430 0.15 -0.6 452,560 - MMP_RS02430 0.15 +0.5 452,601 + MMP_RS02430 0.17 -0.8 452,760 + MMP_RS02430 0.27 +0.0 452,791 - MMP_RS02430 0.29 +4.1 452,792 + MMP_RS02430 0.29 -0.4 452,862 - MMP_RS02430 0.33 +0.3 453,079 + MMP_RS02430 0.47 -1.5 453,313 + MMP_RS02430 0.61 -0.9 453,313 + MMP_RS02430 0.61 +0.8 453,451 - MMP_RS02430 0.69 +0.5 453,452 + MMP_RS02430 0.69 -1.5 453,459 + MMP_RS02430 0.70 +0.2 453,459 + MMP_RS02430 0.70 +4.9 453,463 - MMP_RS02430 0.70 +0.1 453,633 + MMP_RS02430 0.80 -0.2 453,670 - MMP_RS02430 0.82 +0.8 453,671 + MMP_RS02430 0.82 +0.6 453,676 - MMP_RS02430 0.83 +0.6 453,677 + MMP_RS02430 0.83 +0.8 453,693 - MMP_RS02430 0.84 +0.0 453,693 - MMP_RS02430 0.84 +0.3 453,722 - MMP_RS02430 0.86 +0.4 453,734 - MMP_RS02430 0.86 -0.7 453,735 + MMP_RS02430 0.86 +0.1 453,745 - MMP_RS02430 0.87 +0.6 453,858 + +4.9 453,928 - +0.3 453,928 - -0.4 453,928 - -0.3 453,931 - +0.0 453,965 + +0.4 453,970 - +0.8 453,971 + -0.9 454,170 - +0.1 454,185 - +1.3 454,196 - +0.4 454,312 + +0.3 454,312 + -0.6 454,314 - +0.2 454,315 + -0.4 454,504 + MMP_RS02440 0.11 -0.6 454,506 + MMP_RS02440 0.12 -0.4 454,506 + MMP_RS02440 0.12 +0.2 454,515 - MMP_RS02440 0.14 +0.3 454,516 + MMP_RS02440 0.15 +0.3 454,573 + MMP_RS02440 0.30 +0.5 454,624 - MMP_RS02440 0.43 -1.1 454,624 - MMP_RS02440 0.43 -0.3 454,841 + +0.2 454,862 - +1.0 454,944 - +0.8 454,944 - -0.1
Or see this region's nucleotide sequence