Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00440 and MMP_RS00445 are separated by 143 nucleotides MMP_RS00445 and MMP_RS00450 are separated by 15 nucleotides MMP_RS00450 and MMP_RS00455 are separated by 194 nucleotides MMP_RS00455 and MMP_RS00460 are separated by 146 nucleotides
MMP_RS00440: MMP_RS00440 - TRAM domain-containing protein, at 87,608 to 87,817
_RS00440
MMP_RS00445: MMP_RS00445 - 7-carboxy-7-deazaguanine synthase QueE, at 87,961 to 88,689
_RS00445
MMP_RS00450: MMP_RS00450 - 4Fe-4S binding protein, at 88,705 to 89,550
_RS00450
MMP_RS00455: MMP_RS00455 - orotate phosphoribosyltransferase-like protein, at 89,745 to 90,362
_RS00455
MMP_RS00460: MMP_RS00460 - glutamine amidotransferase family protein, at 90,509 to 91,600
_RS00460
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 87.821 kb on - strand at 87.829 kb on - strand at 87.832 kb on - strand at 87.832 kb on - strand at 87.833 kb on + strand at 87.835 kb on - strand at 87.836 kb on + strand at 87.836 kb on + strand at 87.837 kb on - strand at 87.838 kb on + strand at 87.838 kb on + strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.839 kb on - strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.840 kb on + strand at 87.847 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.856 kb on - strand at 87.857 kb on + strand at 87.857 kb on + strand at 87.861 kb on - strand at 87.878 kb on - strand at 87.882 kb on - strand at 87.882 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.884 kb on - strand at 87.885 kb on + strand at 88.056 kb on - strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.057 kb on + strand, within MMP_RS00445 at 88.058 kb on - strand, within MMP_RS00445 at 88.058 kb on - strand, within MMP_RS00445 at 88.059 kb on + strand, within MMP_RS00445 at 88.059 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.061 kb on + strand, within MMP_RS00445 at 88.135 kb on - strand, within MMP_RS00445 at 88.136 kb on + strand, within MMP_RS00445 at 88.231 kb on - strand, within MMP_RS00445 at 88.281 kb on - strand, within MMP_RS00445 at 88.347 kb on + strand, within MMP_RS00445 at 88.404 kb on + strand, within MMP_RS00445 at 88.406 kb on + strand, within MMP_RS00445 at 88.551 kb on - strand, within MMP_RS00445 at 88.581 kb on + strand, within MMP_RS00445 at 88.590 kb on - strand, within MMP_RS00445 at 88.591 kb on + strand, within MMP_RS00445 at 88.608 kb on + strand, within MMP_RS00445 at 88.636 kb on - strand at 88.637 kb on + strand at 88.844 kb on - strand, within MMP_RS00450 at 89.338 kb on + strand, within MMP_RS00450 at 89.534 kb on + strand at 89.553 kb on - strand at 89.560 kb on - strand at 89.561 kb on + strand at 89.565 kb on - strand at 89.618 kb on - strand at 89.619 kb on + strand at 89.620 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.662 kb on - strand at 89.662 kb on - strand at 89.662 kb on - strand at 89.663 kb on + strand at 89.663 kb on + strand at 89.663 kb on + strand at 89.699 kb on - strand at 89.700 kb on + strand at 89.712 kb on - strand at 89.723 kb on - strand at 89.926 kb on - strand, within MMP_RS00455 at 89.969 kb on - strand, within MMP_RS00455 at 90.089 kb on - strand, within MMP_RS00455 at 90.089 kb on - strand, within MMP_RS00455 at 90.090 kb on + strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.094 kb on + strand, within MMP_RS00455 at 90.186 kb on + strand, within MMP_RS00455 at 90.319 kb on - strand at 90.478 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 87,821 - -2.7 87,829 - -1.3 87,832 - -0.6 87,832 - -2.1 87,833 + +0.2 87,835 - -2.9 87,836 + -2.1 87,836 + -1.9 87,837 - -2.2 87,838 + -2.1 87,838 + -3.4 87,839 - +0.1 87,839 - -1.5 87,839 - -0.9 87,839 - -3.0 87,840 + -1.7 87,840 + -2.7 87,840 + -3.3 87,840 + -1.9 87,840 + -0.3 87,847 - -4.2 87,856 - -2.3 87,856 - -2.2 87,856 - -4.0 87,856 - -3.3 87,857 + -1.9 87,857 + -2.0 87,861 - -2.3 87,878 - -3.8 87,882 - -0.7 87,882 - -2.3 87,884 - -0.8 87,884 - -0.1 87,884 - -0.3 87,884 - -0.7 87,884 - +0.0 87,885 + -1.5 88,056 - MMP_RS00445 0.13 -0.1 88,057 + MMP_RS00445 0.13 -0.6 88,057 + MMP_RS00445 0.13 -0.7 88,057 + MMP_RS00445 0.13 -0.7 88,058 - MMP_RS00445 0.13 -0.1 88,058 - MMP_RS00445 0.13 -0.9 88,059 + MMP_RS00445 0.13 -1.1 88,059 + MMP_RS00445 0.13 -0.4 88,061 + MMP_RS00445 0.14 +0.4 88,061 + MMP_RS00445 0.14 -0.3 88,061 + MMP_RS00445 0.14 +4.4 88,135 - MMP_RS00445 0.24 +0.9 88,136 + MMP_RS00445 0.24 +0.1 88,231 - MMP_RS00445 0.37 +0.2 88,281 - MMP_RS00445 0.44 -0.4 88,347 + MMP_RS00445 0.53 -0.7 88,404 + MMP_RS00445 0.61 -0.3 88,406 + MMP_RS00445 0.61 +0.3 88,551 - MMP_RS00445 0.81 +0.5 88,581 + MMP_RS00445 0.85 +1.1 88,590 - MMP_RS00445 0.86 +0.9 88,591 + MMP_RS00445 0.86 +0.5 88,608 + MMP_RS00445 0.89 -0.1 88,636 - +0.1 88,637 + -0.4 88,844 - MMP_RS00450 0.16 +0.5 89,338 + MMP_RS00450 0.75 -2.4 89,534 + -3.0 89,553 - -1.2 89,560 - -1.3 89,561 + -2.0 89,565 - -1.8 89,618 - +3.0 89,619 + -3.5 89,620 - -0.2 89,660 - +0.9 89,660 - -0.1 89,660 - -0.1 89,660 - +0.1 89,660 - -0.0 89,661 + -0.9 89,661 + +0.5 89,661 + -0.1 89,661 + +0.6 89,662 - -0.0 89,662 - +1.1 89,662 - +0.9 89,663 + +1.3 89,663 + +0.6 89,663 + -0.3 89,699 - -0.4 89,700 + -0.7 89,712 - +0.8 89,723 - +0.3 89,926 - MMP_RS00455 0.29 -0.3 89,969 - MMP_RS00455 0.36 -0.6 90,089 - MMP_RS00455 0.56 -0.6 90,089 - MMP_RS00455 0.56 -0.6 90,090 + MMP_RS00455 0.56 +0.9 90,091 - MMP_RS00455 0.56 +0.4 90,091 - MMP_RS00455 0.56 -0.4 90,091 - MMP_RS00455 0.56 -0.4 90,093 - MMP_RS00455 0.56 -0.4 90,093 - MMP_RS00455 0.56 -0.2 90,093 - MMP_RS00455 0.56 -0.2 90,094 + MMP_RS00455 0.56 +4.2 90,186 + MMP_RS00455 0.71 +0.2 90,319 - -0.5 90,478 - -1.0
Or see this region's nucleotide sequence