Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00315 and MMP_RS00320 overlap by 4 nucleotides MMP_RS00320 and MMP_RS00325 are separated by 13 nucleotides MMP_RS00325 and MMP_RS00330 are separated by 107 nucleotides
MMP_RS00315: MMP_RS00315 - Nif3-like dinuclear metal center hexameric protein, at 68,156 to 68,896
_RS00315
MMP_RS00320: MMP_RS00320 - class I SAM-dependent methyltransferase, at 68,893 to 69,501
_RS00320
MMP_RS00325: MMP_RS00325 - RNA-binding protein, at 69,515 to 69,994
_RS00325
MMP_RS00330: MMP_RS00330 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 70,102 to 71,181
_RS00330
Position (kb) 69
70 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 68.613 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.754 kb on + strand, within MMP_RS00315 at 69.287 kb on + strand, within MMP_RS00320 at 69.302 kb on + strand, within MMP_RS00320 at 69.446 kb on - strand at 69.447 kb on + strand at 69.448 kb on - strand at 69.454 kb on + strand at 69.749 kb on - strand, within MMP_RS00325 at 69.750 kb on + strand, within MMP_RS00325 at 70.059 kb on - strand at 70.103 kb on - strand at 70.104 kb on + strand at 70.576 kb on + strand at 70.576 kb on + strand at 70.623 kb on - strand at 70.849 kb on + strand at 70.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 68,613 - MMP_RS00315 0.62 -1.5 68,711 - MMP_RS00315 0.75 -0.6 68,711 - MMP_RS00315 0.75 -2.1 68,754 + MMP_RS00315 0.81 -0.8 69,287 + MMP_RS00320 0.65 -3.4 69,302 + MMP_RS00320 0.67 -1.4 69,446 - -1.5 69,447 + +1.6 69,448 - -1.9 69,454 + -1.0 69,749 - MMP_RS00325 0.49 -2.0 69,750 + MMP_RS00325 0.49 -3.6 70,059 - -0.5 70,103 - -0.2 70,104 + -0.1 70,576 + -4.0 70,576 + -2.2 70,623 - -2.2 70,849 + -2.9 70,906 + -2.5
Or see this region's nucleotide sequence