Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS17995

Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS17985 and HSERO_RS17990 are separated by 130 nucleotidesHSERO_RS17990 and HSERO_RS17995 are separated by 109 nucleotidesHSERO_RS17995 and HSERO_RS18000 are separated by 52 nucleotides HSERO_RS17985: HSERO_RS17985 - tRNA pseudouridine synthase B, at 4,129,881 to 4,130,885 _RS17985 HSERO_RS17990: HSERO_RS17990 - ribosome-binding factor A, at 4,131,016 to 4,131,408 _RS17990 HSERO_RS17995: HSERO_RS17995 - translation initiation factor IF-2, at 4,131,518 to 4,134,382 _RS17995 HSERO_RS18000: HSERO_RS18000 - transcription termination factor NusA, at 4,134,435 to 4,135,994 _RS18000 Position (kb) 4131 4132 4133 4134 4135Strain fitness (log2 ratio) -2 -1 0 1at 4130.528 kb on + strand, within HSERO_RS17985at 4130.637 kb on - strand, within HSERO_RS17985at 4130.663 kb on + strand, within HSERO_RS17985at 4130.731 kb on + strand, within HSERO_RS17985at 4134.420 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
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4,130,528 + HSERO_RS17985 0.64 -1.4
4,130,637 - HSERO_RS17985 0.75 +0.5
4,130,663 + HSERO_RS17985 0.78 -2.1
4,130,731 + HSERO_RS17985 0.85 +0.3
4,134,420 - -1.2

Or see this region's nucleotide sequence