Experiment: Recovery broth with Ticarcillin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13700 and BBR_RS13705 are separated by 68 nucleotides BBR_RS13705 and BBR_RS13710 are separated by 126 nucleotides BBR_RS13710 and BBR_RS13715 are separated by 56 nucleotides BBR_RS13715 and BBR_RS13720 are separated by 105 nucleotides
BBR_RS13700: BBR_RS13700 - diaminopimelate epimerase, at 847,111 to 848,010
_RS13700
BBR_RS13705: BBR_RS13705 - Conserved hypothetical membrane spanning protein, contains a vitamin K epoxide reductase domain, at 848,079 to 848,798
_RS13705
BBR_RS13710: BBR_RS13710 - PHP domain-containing protein, at 848,925 to 849,818
_RS13710
BBR_RS13715: BBR_RS13715 - DUF3107 domain-containing protein, at 849,875 to 850,099
_RS13715
BBR_RS13720: BBR_RS13720 - hypothetical protein, at 850,205 to 851,836
_RS13720
Position (kb) 848
849
850 Strain fitness (log2 ratio) -2
-1
0
1 at 847.925 kb on + strand at 847.952 kb on + strand at 847.953 kb on + strand at 847.974 kb on - strand at 847.993 kb on + strand at 848.003 kb on + strand at 848.006 kb on - strand at 848.020 kb on + strand at 848.101 kb on - strand at 848.103 kb on - strand at 848.145 kb on - strand at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.321 kb on - strand, within BBR_RS13705 at 848.350 kb on + strand, within BBR_RS13705 at 848.368 kb on - strand, within BBR_RS13705 at 848.387 kb on - strand, within BBR_RS13705 at 848.388 kb on + strand, within BBR_RS13705 at 848.391 kb on - strand, within BBR_RS13705 at 848.396 kb on - strand, within BBR_RS13705 at 848.481 kb on - strand, within BBR_RS13705 at 848.505 kb on - strand, within BBR_RS13705 at 848.610 kb on + strand, within BBR_RS13705 at 848.618 kb on - strand, within BBR_RS13705 at 848.694 kb on + strand, within BBR_RS13705 at 848.707 kb on - strand, within BBR_RS13705 at 848.721 kb on + strand, within BBR_RS13705 at 848.728 kb on - strand at 848.819 kb on - strand at 848.927 kb on + strand at 848.938 kb on - strand at 848.940 kb on + strand at 848.940 kb on - strand at 848.940 kb on - strand at 848.953 kb on + strand at 848.953 kb on + strand at 848.963 kb on + strand at 848.971 kb on - strand at 848.971 kb on - strand at 849.041 kb on + strand, within BBR_RS13710 at 849.041 kb on + strand, within BBR_RS13710 at 849.041 kb on + strand, within BBR_RS13710 at 849.041 kb on + strand, within BBR_RS13710 at 849.049 kb on - strand, within BBR_RS13710 at 849.049 kb on - strand, within BBR_RS13710 at 849.049 kb on - strand, within BBR_RS13710 at 849.064 kb on - strand, within BBR_RS13710 at 849.064 kb on - strand, within BBR_RS13710 at 849.064 kb on - strand, within BBR_RS13710 at 849.095 kb on + strand, within BBR_RS13710 at 849.101 kb on + strand, within BBR_RS13710 at 849.101 kb on + strand, within BBR_RS13710 at 849.169 kb on + strand, within BBR_RS13710 at 849.172 kb on + strand, within BBR_RS13710 at 849.199 kb on + strand, within BBR_RS13710 at 849.271 kb on - strand, within BBR_RS13710 at 849.305 kb on + strand, within BBR_RS13710 at 849.305 kb on + strand, within BBR_RS13710 at 849.338 kb on + strand, within BBR_RS13710 at 849.338 kb on + strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.358 kb on + strand, within BBR_RS13710 at 849.358 kb on + strand, within BBR_RS13710 at 849.367 kb on - strand, within BBR_RS13710 at 849.369 kb on - strand, within BBR_RS13710 at 849.369 kb on - strand, within BBR_RS13710 at 849.370 kb on - strand, within BBR_RS13710 at 849.476 kb on + strand, within BBR_RS13710 at 849.476 kb on + strand, within BBR_RS13710 at 849.531 kb on - strand, within BBR_RS13710 at 849.549 kb on + strand, within BBR_RS13710 at 849.617 kb on - strand, within BBR_RS13710 at 849.619 kb on + strand, within BBR_RS13710 at 849.627 kb on + strand, within BBR_RS13710 at 849.646 kb on + strand, within BBR_RS13710 at 849.654 kb on - strand, within BBR_RS13710 at 849.664 kb on - strand, within BBR_RS13710 at 849.682 kb on + strand, within BBR_RS13710 at 849.717 kb on - strand, within BBR_RS13710 at 849.718 kb on + strand, within BBR_RS13710 at 849.726 kb on - strand, within BBR_RS13710 at 849.812 kb on - strand at 849.818 kb on + strand at 849.819 kb on + strand at 849.848 kb on + strand at 849.856 kb on - strand at 849.863 kb on - strand at 849.872 kb on + strand at 849.872 kb on + strand at 849.874 kb on + strand at 849.882 kb on - strand at 849.929 kb on + strand, within BBR_RS13715 at 849.937 kb on - strand, within BBR_RS13715 at 849.944 kb on - strand, within BBR_RS13715 at 849.999 kb on + strand, within BBR_RS13715 at 850.006 kb on - strand, within BBR_RS13715 at 850.055 kb on - strand, within BBR_RS13715 at 850.105 kb on - strand at 850.115 kb on - strand at 850.117 kb on - strand at 850.174 kb on - strand at 850.253 kb on - strand at 850.258 kb on - strand at 850.277 kb on - strand at 850.277 kb on - strand at 850.277 kb on - strand at 850.300 kb on - strand at 850.300 kb on - strand at 850.316 kb on - strand at 850.362 kb on + strand at 850.401 kb on + strand, within BBR_RS13720 at 850.401 kb on + strand, within BBR_RS13720 at 850.401 kb on + strand, within BBR_RS13720 at 850.409 kb on - strand, within BBR_RS13720 at 850.409 kb on - strand, within BBR_RS13720 at 850.495 kb on - strand, within BBR_RS13720 at 850.540 kb on + strand, within BBR_RS13720 at 850.545 kb on + strand, within BBR_RS13720 at 850.553 kb on - strand, within BBR_RS13720 at 850.553 kb on - strand, within BBR_RS13720 at 850.602 kb on + strand, within BBR_RS13720 at 850.602 kb on + strand, within BBR_RS13720 at 850.604 kb on - strand, within BBR_RS13720 at 850.604 kb on - strand, within BBR_RS13720 at 850.681 kb on + strand, within BBR_RS13720 at 850.689 kb on - strand, within BBR_RS13720 at 850.689 kb on - strand, within BBR_RS13720 at 850.701 kb on - strand, within BBR_RS13720 at 850.701 kb on - strand, within BBR_RS13720 at 850.724 kb on - strand, within BBR_RS13720 at 850.806 kb on + strand, within BBR_RS13720 at 850.806 kb on + strand, within BBR_RS13720
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 2e-04 mM remove 847,925 + -0.2 847,952 + -0.4 847,953 + +0.3 847,974 - -1.0 847,993 + -0.7 848,003 + -0.4 848,006 - -0.0 848,020 + -1.1 848,101 - +0.4 848,103 - -0.2 848,145 - +0.2 848,291 - BBR_RS13705 0.29 +0.4 848,291 - BBR_RS13705 0.29 -0.0 848,291 - BBR_RS13705 0.29 +0.4 848,305 + BBR_RS13705 0.31 +0.3 848,305 + BBR_RS13705 0.31 +0.5 848,321 - BBR_RS13705 0.34 -0.3 848,350 + BBR_RS13705 0.38 -0.2 848,368 - BBR_RS13705 0.40 +0.3 848,387 - BBR_RS13705 0.43 +1.2 848,388 + BBR_RS13705 0.43 -0.3 848,391 - BBR_RS13705 0.43 -0.0 848,396 - BBR_RS13705 0.44 -1.4 848,481 - BBR_RS13705 0.56 -0.8 848,505 - BBR_RS13705 0.59 +0.4 848,610 + BBR_RS13705 0.74 +0.7 848,618 - BBR_RS13705 0.75 -0.5 848,694 + BBR_RS13705 0.85 +0.2 848,707 - BBR_RS13705 0.87 +0.3 848,721 + BBR_RS13705 0.89 -0.2 848,728 - -1.4 848,819 - -0.0 848,927 + +0.1 848,938 - +0.1 848,940 + +0.8 848,940 - -0.1 848,940 - -0.0 848,953 + +0.3 848,953 + -1.7 848,963 + -0.1 848,971 - -0.6 848,971 - -0.3 849,041 + BBR_RS13710 0.13 +0.1 849,041 + BBR_RS13710 0.13 +0.3 849,041 + BBR_RS13710 0.13 -0.3 849,041 + BBR_RS13710 0.13 -0.2 849,049 - BBR_RS13710 0.14 -0.5 849,049 - BBR_RS13710 0.14 -0.2 849,049 - BBR_RS13710 0.14 +0.8 849,064 - BBR_RS13710 0.16 -0.2 849,064 - BBR_RS13710 0.16 +0.3 849,064 - BBR_RS13710 0.16 +0.7 849,095 + BBR_RS13710 0.19 -0.0 849,101 + BBR_RS13710 0.20 -2.1 849,101 + BBR_RS13710 0.20 +0.5 849,169 + BBR_RS13710 0.27 +0.0 849,172 + BBR_RS13710 0.28 -0.3 849,199 + BBR_RS13710 0.31 -0.2 849,271 - BBR_RS13710 0.39 +0.7 849,305 + BBR_RS13710 0.43 -0.2 849,305 + BBR_RS13710 0.43 +0.4 849,338 + BBR_RS13710 0.46 +0.5 849,338 + BBR_RS13710 0.46 -0.3 849,346 - BBR_RS13710 0.47 -0.3 849,346 - BBR_RS13710 0.47 +0.8 849,346 - BBR_RS13710 0.47 +0.1 849,358 + BBR_RS13710 0.48 +0.4 849,358 + BBR_RS13710 0.48 +0.6 849,367 - BBR_RS13710 0.49 -2.0 849,369 - BBR_RS13710 0.50 +0.4 849,369 - BBR_RS13710 0.50 -0.8 849,370 - BBR_RS13710 0.50 -0.2 849,476 + BBR_RS13710 0.62 -1.0 849,476 + BBR_RS13710 0.62 -0.1 849,531 - BBR_RS13710 0.68 -0.4 849,549 + BBR_RS13710 0.70 +0.1 849,617 - BBR_RS13710 0.77 -0.2 849,619 + BBR_RS13710 0.78 +0.2 849,627 + BBR_RS13710 0.79 +0.7 849,646 + BBR_RS13710 0.81 +0.2 849,654 - BBR_RS13710 0.82 +0.5 849,664 - BBR_RS13710 0.83 -0.5 849,682 + BBR_RS13710 0.85 -0.6 849,717 - BBR_RS13710 0.89 -0.6 849,718 + BBR_RS13710 0.89 -0.3 849,726 - BBR_RS13710 0.90 +0.3 849,812 - -0.6 849,818 + -0.2 849,819 + -0.6 849,848 + -2.1 849,856 - -0.1 849,863 - -1.5 849,872 + -0.6 849,872 + -0.3 849,874 + +0.2 849,882 - +0.3 849,929 + BBR_RS13715 0.24 +0.2 849,937 - BBR_RS13715 0.28 -0.0 849,944 - BBR_RS13715 0.31 +0.1 849,999 + BBR_RS13715 0.55 -0.8 850,006 - BBR_RS13715 0.58 +0.2 850,055 - BBR_RS13715 0.80 +0.5 850,105 - -0.0 850,115 - +1.2 850,117 - -0.4 850,174 - -0.4 850,253 - -1.0 850,258 - -1.3 850,277 - -0.8 850,277 - +0.1 850,277 - -0.2 850,300 - -0.8 850,300 - -1.4 850,316 - -0.7 850,362 + +0.3 850,401 + BBR_RS13720 0.12 -1.0 850,401 + BBR_RS13720 0.12 -0.4 850,401 + BBR_RS13720 0.12 -0.1 850,409 - BBR_RS13720 0.12 -0.6 850,409 - BBR_RS13720 0.12 -0.3 850,495 - BBR_RS13720 0.18 -1.9 850,540 + BBR_RS13720 0.21 -0.0 850,545 + BBR_RS13720 0.21 -1.0 850,553 - BBR_RS13720 0.21 -1.5 850,553 - BBR_RS13720 0.21 -2.2 850,602 + BBR_RS13720 0.24 -2.0 850,602 + BBR_RS13720 0.24 -0.3 850,604 - BBR_RS13720 0.24 -2.2 850,604 - BBR_RS13720 0.24 -0.6 850,681 + BBR_RS13720 0.29 +0.0 850,689 - BBR_RS13720 0.30 -1.2 850,689 - BBR_RS13720 0.30 -1.4 850,701 - BBR_RS13720 0.30 -1.9 850,701 - BBR_RS13720 0.30 +0.3 850,724 - BBR_RS13720 0.32 -2.7 850,806 + BBR_RS13720 0.37 +0.6 850,806 + BBR_RS13720 0.37 -0.8
Or see this region's nucleotide sequence