Experiment: Nalidixic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10715 and ABZR86_RS10720 are separated by 218 nucleotides ABZR86_RS10720 and ABZR86_RS10725 are separated by 201 nucleotides
ABZR86_RS10715: ABZR86_RS10715 - serine hydroxymethyltransferase, at 2,380,008 to 2,381,261
_RS10715
ABZR86_RS10720: ABZR86_RS10720 - DUF817 domain-containing protein, at 2,381,480 to 2,382,373
_RS10720
ABZR86_RS10725: ABZR86_RS10725 - energy-dependent translational throttle protein EttA, at 2,382,575 to 2,384,236
_RS10725
Position (kb)
2381
2382
2383 Strain fitness (log2 ratio)
-1
0
1
2 at 2380.624 kb on + strand, within ABZR86_RS10715 at 2380.624 kb on + strand, within ABZR86_RS10715 at 2380.675 kb on + strand, within ABZR86_RS10715 at 2380.675 kb on + strand, within ABZR86_RS10715 at 2380.675 kb on + strand, within ABZR86_RS10715 at 2380.676 kb on - strand, within ABZR86_RS10715 at 2380.676 kb on - strand, within ABZR86_RS10715 at 2380.733 kb on - strand, within ABZR86_RS10715 at 2380.798 kb on + strand, within ABZR86_RS10715 at 2380.799 kb on - strand, within ABZR86_RS10715 at 2380.799 kb on - strand, within ABZR86_RS10715 at 2380.828 kb on + strand, within ABZR86_RS10715 at 2380.829 kb on - strand, within ABZR86_RS10715 at 2380.945 kb on + strand, within ABZR86_RS10715 at 2380.946 kb on - strand, within ABZR86_RS10715 at 2380.946 kb on - strand, within ABZR86_RS10715 at 2380.946 kb on - strand, within ABZR86_RS10715 at 2380.946 kb on - strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.993 kb on + strand, within ABZR86_RS10715 at 2380.994 kb on - strand, within ABZR86_RS10715 at 2380.994 kb on - strand, within ABZR86_RS10715 at 2380.994 kb on - strand, within ABZR86_RS10715 at 2380.994 kb on - strand, within ABZR86_RS10715 at 2381.053 kb on + strand, within ABZR86_RS10715 at 2381.054 kb on - strand, within ABZR86_RS10715 at 2381.054 kb on - strand, within ABZR86_RS10715 at 2381.068 kb on + strand, within ABZR86_RS10715 at 2381.068 kb on + strand, within ABZR86_RS10715 at 2381.068 kb on + strand, within ABZR86_RS10715 at 2381.071 kb on + strand, within ABZR86_RS10715 at 2381.071 kb on + strand, within ABZR86_RS10715 at 2381.072 kb on - strand, within ABZR86_RS10715 at 2381.072 kb on - strand, within ABZR86_RS10715 at 2381.098 kb on + strand, within ABZR86_RS10715 at 2381.099 kb on - strand, within ABZR86_RS10715 at 2381.149 kb on + strand at 2381.149 kb on + strand at 2381.149 kb on + strand at 2381.149 kb on + strand at 2381.334 kb on + strand at 2381.334 kb on + strand at 2381.334 kb on + strand at 2381.334 kb on + strand at 2381.335 kb on - strand at 2381.335 kb on - strand at 2381.335 kb on - strand at 2381.335 kb on - strand at 2381.515 kb on + strand at 2381.544 kb on + strand at 2381.544 kb on + strand at 2381.545 kb on - strand at 2381.545 kb on - strand at 2381.827 kb on - strand, within ABZR86_RS10720 at 2381.911 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.913 kb on + strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.914 kb on - strand, within ABZR86_RS10720 at 2381.931 kb on + strand, within ABZR86_RS10720 at 2381.931 kb on + strand, within ABZR86_RS10720 at 2381.931 kb on + strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2381.932 kb on - strand, within ABZR86_RS10720 at 2382.339 kb on - strand at 2382.398 kb on - strand at 2382.500 kb on - strand at 2382.581 kb on + strand at 2382.581 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.01 mg/ml remove 2,380,624 + ABZR86_RS10715 0.49 +0.7 2,380,624 + ABZR86_RS10715 0.49 +0.7 2,380,675 + ABZR86_RS10715 0.53 +0.2 2,380,675 + ABZR86_RS10715 0.53 -0.3 2,380,675 + ABZR86_RS10715 0.53 +0.7 2,380,676 - ABZR86_RS10715 0.53 +0.6 2,380,676 - ABZR86_RS10715 0.53 +0.4 2,380,733 - ABZR86_RS10715 0.58 +0.8 2,380,798 + ABZR86_RS10715 0.63 +1.5 2,380,799 - ABZR86_RS10715 0.63 +0.8 2,380,799 - ABZR86_RS10715 0.63 -0.1 2,380,828 + ABZR86_RS10715 0.65 -1.1 2,380,829 - ABZR86_RS10715 0.65 +0.2 2,380,945 + ABZR86_RS10715 0.75 +0.5 2,380,946 - ABZR86_RS10715 0.75 +0.3 2,380,946 - ABZR86_RS10715 0.75 -0.1 2,380,946 - ABZR86_RS10715 0.75 -0.1 2,380,946 - ABZR86_RS10715 0.75 +0.8 2,380,993 + ABZR86_RS10715 0.79 +0.4 2,380,993 + ABZR86_RS10715 0.79 +0.3 2,380,993 + ABZR86_RS10715 0.79 -0.1 2,380,993 + ABZR86_RS10715 0.79 +0.2 2,380,993 + ABZR86_RS10715 0.79 +0.2 2,380,993 + ABZR86_RS10715 0.79 -0.2 2,380,993 + ABZR86_RS10715 0.79 +0.1 2,380,993 + ABZR86_RS10715 0.79 -0.1 2,380,993 + ABZR86_RS10715 0.79 +0.5 2,380,994 - ABZR86_RS10715 0.79 +0.2 2,380,994 - ABZR86_RS10715 0.79 +0.3 2,380,994 - ABZR86_RS10715 0.79 +1.8 2,380,994 - ABZR86_RS10715 0.79 +1.5 2,381,053 + ABZR86_RS10715 0.83 +0.1 2,381,054 - ABZR86_RS10715 0.83 -0.1 2,381,054 - ABZR86_RS10715 0.83 -0.1 2,381,068 + ABZR86_RS10715 0.85 -0.4 2,381,068 + ABZR86_RS10715 0.85 +0.6 2,381,068 + ABZR86_RS10715 0.85 -0.7 2,381,071 + ABZR86_RS10715 0.85 +0.4 2,381,071 + ABZR86_RS10715 0.85 +0.4 2,381,072 - ABZR86_RS10715 0.85 +0.5 2,381,072 - ABZR86_RS10715 0.85 +1.4 2,381,098 + ABZR86_RS10715 0.87 +0.8 2,381,099 - ABZR86_RS10715 0.87 +0.3 2,381,149 + +0.6 2,381,149 + +0.3 2,381,149 + +0.2 2,381,149 + -0.0 2,381,334 + +0.8 2,381,334 + -0.2 2,381,334 + +0.0 2,381,334 + -0.2 2,381,335 - -1.2 2,381,335 - -0.2 2,381,335 - +0.6 2,381,335 - +0.5 2,381,515 + +0.4 2,381,544 + -0.1 2,381,544 + +0.3 2,381,545 - -0.0 2,381,545 - -0.1 2,381,827 - ABZR86_RS10720 0.39 +0.3 2,381,911 + ABZR86_RS10720 0.48 -0.1 2,381,913 + ABZR86_RS10720 0.48 +0.1 2,381,913 + ABZR86_RS10720 0.48 -0.1 2,381,913 + ABZR86_RS10720 0.48 -0.3 2,381,913 + ABZR86_RS10720 0.48 -1.3 2,381,913 + ABZR86_RS10720 0.48 +0.2 2,381,913 + ABZR86_RS10720 0.48 -0.5 2,381,914 - ABZR86_RS10720 0.49 +0.5 2,381,914 - ABZR86_RS10720 0.49 -0.0 2,381,914 - ABZR86_RS10720 0.49 +0.7 2,381,914 - ABZR86_RS10720 0.49 +0.4 2,381,914 - ABZR86_RS10720 0.49 +0.2 2,381,914 - ABZR86_RS10720 0.49 +0.3 2,381,914 - ABZR86_RS10720 0.49 -0.5 2,381,914 - ABZR86_RS10720 0.49 -0.3 2,381,914 - ABZR86_RS10720 0.49 -1.0 2,381,914 - ABZR86_RS10720 0.49 -0.0 2,381,914 - ABZR86_RS10720 0.49 +0.9 2,381,931 + ABZR86_RS10720 0.50 -0.1 2,381,931 + ABZR86_RS10720 0.50 -0.4 2,381,931 + ABZR86_RS10720 0.50 -0.4 2,381,932 - ABZR86_RS10720 0.51 -0.5 2,381,932 - ABZR86_RS10720 0.51 -0.3 2,381,932 - ABZR86_RS10720 0.51 +0.0 2,381,932 - ABZR86_RS10720 0.51 -0.2 2,381,932 - ABZR86_RS10720 0.51 -0.3 2,381,932 - ABZR86_RS10720 0.51 -0.2 2,382,339 - +0.7 2,382,398 - +0.6 2,382,500 - -0.0 2,382,581 + -0.6 2,382,581 + -0.0
Or see this region's nucleotide sequence