Experiment: Nalidixic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS06790 and ABZR86_RS06795 are separated by 25 nucleotides ABZR86_RS06795 and ABZR86_RS06800 are separated by 2 nucleotides ABZR86_RS06800 and ABZR86_RS06805 are separated by 113 nucleotides
ABZR86_RS06790: ABZR86_RS06790 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 1,493,064 to 1,494,335
_RS06790
ABZR86_RS06795: ABZR86_RS06795 - GNAT family N-acetyltransferase, at 1,494,361 to 1,494,957
_RS06795
ABZR86_RS06800: ABZR86_RS06800 - ketosteroid isomerase-related protein, at 1,494,960 to 1,495,364
_RS06800
ABZR86_RS06805: ABZR86_RS06805 - acetyl-CoA hydrolase/transferase C-terminal domain-containing protein, at 1,495,478 to 1,497,427
_RS06805
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-2
-1
0
1 at 1494.467 kb on + strand, within ABZR86_RS06795 at 1494.467 kb on + strand, within ABZR86_RS06795 at 1494.467 kb on + strand, within ABZR86_RS06795 at 1494.635 kb on + strand, within ABZR86_RS06795 at 1494.665 kb on + strand, within ABZR86_RS06795 at 1494.665 kb on + strand, within ABZR86_RS06795 at 1494.784 kb on + strand, within ABZR86_RS06795 at 1494.785 kb on - strand, within ABZR86_RS06795 at 1494.785 kb on - strand, within ABZR86_RS06795 at 1494.785 kb on - strand, within ABZR86_RS06795 at 1494.813 kb on - strand, within ABZR86_RS06795 at 1494.824 kb on + strand, within ABZR86_RS06795 at 1494.825 kb on - strand, within ABZR86_RS06795 at 1494.836 kb on + strand, within ABZR86_RS06795 at 1494.837 kb on - strand, within ABZR86_RS06795 at 1494.854 kb on + strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.855 kb on - strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.856 kb on + strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.857 kb on - strand, within ABZR86_RS06795 at 1494.860 kb on + strand, within ABZR86_RS06795 at 1494.861 kb on - strand, within ABZR86_RS06795 at 1494.861 kb on - strand, within ABZR86_RS06795 at 1494.861 kb on - strand, within ABZR86_RS06795 at 1494.861 kb on - strand, within ABZR86_RS06795 at 1494.912 kb on - strand at 1494.997 kb on + strand at 1494.997 kb on + strand at 1494.997 kb on + strand at 1494.997 kb on + strand at 1494.997 kb on + strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1494.998 kb on - strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.000 kb on + strand at 1495.001 kb on - strand, within ABZR86_RS06800 at 1495.001 kb on - strand, within ABZR86_RS06800 at 1495.001 kb on - strand, within ABZR86_RS06800 at 1495.001 kb on - strand, within ABZR86_RS06800 at 1495.063 kb on + strand, within ABZR86_RS06800 at 1495.063 kb on + strand, within ABZR86_RS06800 at 1495.063 kb on + strand, within ABZR86_RS06800 at 1495.063 kb on + strand, within ABZR86_RS06800 at 1495.064 kb on - strand, within ABZR86_RS06800 at 1495.064 kb on - strand, within ABZR86_RS06800 at 1495.108 kb on + strand, within ABZR86_RS06800 at 1495.126 kb on + strand, within ABZR86_RS06800 at 1495.193 kb on - strand, within ABZR86_RS06800 at 1495.330 kb on + strand at 1495.330 kb on + strand at 1495.331 kb on - strand at 1495.331 kb on - strand at 1495.331 kb on - strand at 1495.402 kb on - strand at 1495.402 kb on - strand at 1495.408 kb on + strand at 1495.409 kb on - strand at 1495.944 kb on + strand, within ABZR86_RS06805 at 1495.945 kb on - strand, within ABZR86_RS06805 at 1495.995 kb on + strand, within ABZR86_RS06805 at 1495.996 kb on - strand, within ABZR86_RS06805 at 1496.008 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.103 kb on + strand, within ABZR86_RS06805 at 1496.104 kb on - strand, within ABZR86_RS06805 at 1496.104 kb on - strand, within ABZR86_RS06805 at 1496.104 kb on - strand, within ABZR86_RS06805 at 1496.244 kb on + strand, within ABZR86_RS06805 at 1496.322 kb on + strand, within ABZR86_RS06805 at 1496.323 kb on - strand, within ABZR86_RS06805 at 1496.362 kb on - strand, within ABZR86_RS06805 at 1496.362 kb on - strand, within ABZR86_RS06805 at 1496.362 kb on - strand, within ABZR86_RS06805 at 1496.362 kb on - strand, within ABZR86_RS06805
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.01 mg/ml remove 1,494,467 + ABZR86_RS06795 0.18 -0.9 1,494,467 + ABZR86_RS06795 0.18 -0.6 1,494,467 + ABZR86_RS06795 0.18 -1.4 1,494,635 + ABZR86_RS06795 0.46 +0.3 1,494,665 + ABZR86_RS06795 0.51 -0.1 1,494,665 + ABZR86_RS06795 0.51 -0.1 1,494,784 + ABZR86_RS06795 0.71 -0.2 1,494,785 - ABZR86_RS06795 0.71 +0.2 1,494,785 - ABZR86_RS06795 0.71 +0.1 1,494,785 - ABZR86_RS06795 0.71 +1.1 1,494,813 - ABZR86_RS06795 0.76 -0.0 1,494,824 + ABZR86_RS06795 0.78 +0.5 1,494,825 - ABZR86_RS06795 0.78 +0.0 1,494,836 + ABZR86_RS06795 0.80 +0.0 1,494,837 - ABZR86_RS06795 0.80 -0.1 1,494,854 + ABZR86_RS06795 0.83 +0.0 1,494,855 - ABZR86_RS06795 0.83 +0.2 1,494,855 - ABZR86_RS06795 0.83 +0.2 1,494,855 - ABZR86_RS06795 0.83 +0.1 1,494,855 - ABZR86_RS06795 0.83 -0.9 1,494,855 - ABZR86_RS06795 0.83 -0.7 1,494,855 - ABZR86_RS06795 0.83 -0.1 1,494,855 - ABZR86_RS06795 0.83 -0.2 1,494,855 - ABZR86_RS06795 0.83 +0.4 1,494,856 + ABZR86_RS06795 0.83 -0.5 1,494,856 + ABZR86_RS06795 0.83 +0.2 1,494,856 + ABZR86_RS06795 0.83 -1.6 1,494,856 + ABZR86_RS06795 0.83 +0.5 1,494,856 + ABZR86_RS06795 0.83 +1.0 1,494,856 + ABZR86_RS06795 0.83 -0.8 1,494,856 + ABZR86_RS06795 0.83 -0.8 1,494,856 + ABZR86_RS06795 0.83 +1.6 1,494,856 + ABZR86_RS06795 0.83 -0.9 1,494,857 - ABZR86_RS06795 0.83 -0.4 1,494,857 - ABZR86_RS06795 0.83 -0.1 1,494,857 - ABZR86_RS06795 0.83 -1.0 1,494,857 - ABZR86_RS06795 0.83 -0.0 1,494,857 - ABZR86_RS06795 0.83 -0.0 1,494,857 - ABZR86_RS06795 0.83 +0.9 1,494,857 - ABZR86_RS06795 0.83 -0.4 1,494,857 - ABZR86_RS06795 0.83 +0.5 1,494,857 - ABZR86_RS06795 0.83 +0.1 1,494,857 - ABZR86_RS06795 0.83 -0.7 1,494,857 - ABZR86_RS06795 0.83 +0.1 1,494,860 + ABZR86_RS06795 0.84 +1.1 1,494,861 - ABZR86_RS06795 0.84 -0.6 1,494,861 - ABZR86_RS06795 0.84 +0.5 1,494,861 - ABZR86_RS06795 0.84 -0.3 1,494,861 - ABZR86_RS06795 0.84 -0.0 1,494,912 - +0.0 1,494,997 + +0.1 1,494,997 + -1.5 1,494,997 + +0.2 1,494,997 + +0.2 1,494,997 + -0.1 1,494,998 - +0.5 1,494,998 - -0.1 1,494,998 - -0.4 1,494,998 - -0.1 1,494,998 - +0.5 1,494,998 - +1.5 1,494,998 - +0.4 1,494,998 - -0.3 1,494,998 - -0.2 1,495,000 + -0.1 1,495,000 + -0.5 1,495,000 + +0.3 1,495,000 + -0.8 1,495,000 + +0.2 1,495,000 + +0.4 1,495,000 + +0.2 1,495,000 + +0.4 1,495,001 - ABZR86_RS06800 0.10 -0.9 1,495,001 - ABZR86_RS06800 0.10 -0.2 1,495,001 - ABZR86_RS06800 0.10 +0.7 1,495,001 - ABZR86_RS06800 0.10 +1.0 1,495,063 + ABZR86_RS06800 0.25 -0.6 1,495,063 + ABZR86_RS06800 0.25 +0.7 1,495,063 + ABZR86_RS06800 0.25 -0.5 1,495,063 + ABZR86_RS06800 0.25 +0.7 1,495,064 - ABZR86_RS06800 0.26 +0.5 1,495,064 - ABZR86_RS06800 0.26 -0.4 1,495,108 + ABZR86_RS06800 0.37 -0.6 1,495,126 + ABZR86_RS06800 0.41 +0.5 1,495,193 - ABZR86_RS06800 0.58 +1.1 1,495,330 + -1.5 1,495,330 + +0.2 1,495,331 - -0.0 1,495,331 - -0.3 1,495,331 - -0.5 1,495,402 - +0.5 1,495,402 - +0.5 1,495,408 + +0.5 1,495,409 - -0.7 1,495,944 + ABZR86_RS06805 0.24 +0.5 1,495,945 - ABZR86_RS06805 0.24 +0.6 1,495,995 + ABZR86_RS06805 0.27 +0.0 1,495,996 - ABZR86_RS06805 0.27 -0.2 1,496,008 + ABZR86_RS06805 0.27 +0.4 1,496,103 + ABZR86_RS06805 0.32 +0.2 1,496,103 + ABZR86_RS06805 0.32 +0.6 1,496,103 + ABZR86_RS06805 0.32 +0.7 1,496,103 + ABZR86_RS06805 0.32 +0.2 1,496,103 + ABZR86_RS06805 0.32 -0.1 1,496,103 + ABZR86_RS06805 0.32 -2.0 1,496,103 + ABZR86_RS06805 0.32 -0.6 1,496,104 - ABZR86_RS06805 0.32 -0.1 1,496,104 - ABZR86_RS06805 0.32 -0.4 1,496,104 - ABZR86_RS06805 0.32 +0.3 1,496,244 + ABZR86_RS06805 0.39 +0.7 1,496,322 + ABZR86_RS06805 0.43 -0.6 1,496,323 - ABZR86_RS06805 0.43 +1.7 1,496,362 - ABZR86_RS06805 0.45 +0.2 1,496,362 - ABZR86_RS06805 0.45 +0.3 1,496,362 - ABZR86_RS06805 0.45 -0.0 1,496,362 - ABZR86_RS06805 0.45 -0.2
Or see this region's nucleotide sequence