Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_918 and Pf1N1B4_919 are separated by 46 nucleotides Pf1N1B4_919 and Pf1N1B4_920 are separated by 6 nucleotides Pf1N1B4_920 and Pf1N1B4_921 are separated by 139 nucleotides
Pf1N1B4_918 - phospholipase/carboxylesterase family protein, at 1,043,850 to 1,044,506
_918
Pf1N1B4_919 - hypothetical protein, at 1,044,553 to 1,044,741
_919
Pf1N1B4_920 - ATP-dependent RNA helicase RhlB, at 1,044,748 to 1,046,079
_920
Pf1N1B4_921 - Ornithine cyclodeaminase (EC 4.3.1.12), at 1,046,219 to 1,047,166
_921
Position (kb)
1044
1045
1046
1047 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1043.780 kb on + strand at 1043.781 kb on - strand at 1043.794 kb on - strand at 1043.794 kb on - strand at 1043.794 kb on - strand at 1043.817 kb on + strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.993 kb on - strand, within Pf1N1B4_918 at 1044.051 kb on + strand, within Pf1N1B4_918 at 1044.058 kb on + strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.158 kb on - strand, within Pf1N1B4_918 at 1044.158 kb on - strand, within Pf1N1B4_918 at 1044.208 kb on + strand, within Pf1N1B4_918 at 1044.209 kb on - strand, within Pf1N1B4_918 at 1044.209 kb on - strand, within Pf1N1B4_918 at 1044.253 kb on + strand, within Pf1N1B4_918 at 1044.253 kb on + strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.270 kb on + strand, within Pf1N1B4_918 at 1044.270 kb on + strand, within Pf1N1B4_918 at 1044.271 kb on - strand, within Pf1N1B4_918 at 1044.271 kb on - strand, within Pf1N1B4_918 at 1044.348 kb on + strand at 1044.348 kb on + strand, within Pf1N1B4_918 at 1044.349 kb on - strand, within Pf1N1B4_918 at 1044.394 kb on - strand, within Pf1N1B4_918 at 1044.397 kb on + strand, within Pf1N1B4_918 at 1044.397 kb on + strand, within Pf1N1B4_918 at 1044.397 kb on + strand, within Pf1N1B4_918 at 1044.397 kb on + strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.398 kb on - strand at 1044.398 kb on - strand, within Pf1N1B4_918 at 1044.512 kb on - strand at 1044.512 kb on - strand at 1044.532 kb on - strand at 1044.532 kb on - strand at 1044.554 kb on + strand at 1044.554 kb on + strand at 1044.554 kb on + strand at 1044.555 kb on - strand at 1044.555 kb on - strand at 1044.555 kb on - strand at 1044.555 kb on - strand at 1044.555 kb on - strand at 1044.555 kb on - strand at 1044.568 kb on + strand at 1044.569 kb on - strand at 1044.569 kb on - strand at 1044.569 kb on - strand at 1044.569 kb on - strand at 1044.570 kb on + strand at 1044.571 kb on - strand at 1044.571 kb on - strand at 1044.571 kb on - strand at 1044.571 kb on - strand at 1044.571 kb on - strand at 1044.571 kb on - strand at 1044.628 kb on - strand, within Pf1N1B4_919 at 1044.628 kb on - strand, within Pf1N1B4_919 at 1044.675 kb on + strand, within Pf1N1B4_919 at 1044.675 kb on + strand, within Pf1N1B4_919 at 1044.974 kb on - strand, within Pf1N1B4_920 at 1045.123 kb on + strand, within Pf1N1B4_920 at 1045.123 kb on + strand, within Pf1N1B4_920 at 1045.123 kb on + strand, within Pf1N1B4_920 at 1045.124 kb on - strand, within Pf1N1B4_920 at 1045.124 kb on - strand, within Pf1N1B4_920 at 1045.124 kb on - strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand, within Pf1N1B4_920 at 1045.210 kb on + strand at 1045.211 kb on - strand, within Pf1N1B4_920 at 1045.211 kb on - strand, within Pf1N1B4_920 at 1045.211 kb on - strand, within Pf1N1B4_920 at 1045.211 kb on - strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.591 kb on + strand, within Pf1N1B4_920 at 1045.592 kb on - strand, within Pf1N1B4_920 at 1045.592 kb on - strand, within Pf1N1B4_920 at 1045.592 kb on - strand, within Pf1N1B4_920 at 1045.592 kb on - strand, within Pf1N1B4_920 at 1045.615 kb on + strand, within Pf1N1B4_920 at 1045.615 kb on + strand, within Pf1N1B4_920 at 1045.615 kb on + strand, within Pf1N1B4_920 at 1045.615 kb on + strand, within Pf1N1B4_920 at 1045.616 kb on - strand, within Pf1N1B4_920 at 1045.616 kb on - strand, within Pf1N1B4_920 at 1045.616 kb on - strand, within Pf1N1B4_920 at 1045.616 kb on - strand, within Pf1N1B4_920 at 1045.627 kb on + strand, within Pf1N1B4_920 at 1045.627 kb on + strand, within Pf1N1B4_920 at 1045.628 kb on - strand, within Pf1N1B4_920 at 1045.715 kb on + strand, within Pf1N1B4_920 at 1045.715 kb on + strand, within Pf1N1B4_920 at 1045.716 kb on - strand, within Pf1N1B4_920 at 1045.716 kb on - strand, within Pf1N1B4_920 at 1045.716 kb on - strand, within Pf1N1B4_920 at 1045.716 kb on - strand, within Pf1N1B4_920 at 1045.903 kb on + strand, within Pf1N1B4_920 at 1045.903 kb on + strand, within Pf1N1B4_920 at 1045.903 kb on + strand, within Pf1N1B4_920 at 1045.903 kb on + strand, within Pf1N1B4_920 at 1045.904 kb on - strand, within Pf1N1B4_920 at 1045.904 kb on - strand, within Pf1N1B4_920 at 1045.904 kb on - strand, within Pf1N1B4_920 at 1045.982 kb on - strand at 1046.191 kb on - strand at 1046.234 kb on + strand at 1046.234 kb on + strand at 1046.235 kb on - strand at 1046.235 kb on - strand at 1046.235 kb on - strand at 1046.235 kb on - strand at 1046.235 kb on - strand at 1046.390 kb on + strand, within Pf1N1B4_921 at 1046.390 kb on + strand, within Pf1N1B4_921 at 1046.390 kb on + strand, within Pf1N1B4_921 at 1046.390 kb on + strand, within Pf1N1B4_921 at 1046.391 kb on - strand, within Pf1N1B4_921 at 1046.391 kb on - strand, within Pf1N1B4_921 at 1046.391 kb on - strand, within Pf1N1B4_921 at 1046.391 kb on - strand, within Pf1N1B4_921 at 1046.420 kb on + strand, within Pf1N1B4_921 at 1046.421 kb on - strand, within Pf1N1B4_921 at 1046.421 kb on - strand, within Pf1N1B4_921 at 1046.421 kb on - strand, within Pf1N1B4_921 at 1046.421 kb on - strand, within Pf1N1B4_921 at 1046.421 kb on - strand, within Pf1N1B4_921 at 1046.441 kb on + strand, within Pf1N1B4_921 at 1046.441 kb on + strand, within Pf1N1B4_921 at 1046.442 kb on - strand, within Pf1N1B4_921 at 1046.442 kb on - strand at 1046.655 kb on - strand, within Pf1N1B4_921 at 1046.655 kb on - strand, within Pf1N1B4_921 at 1046.655 kb on - strand, within Pf1N1B4_921 at 1046.655 kb on - strand, within Pf1N1B4_921 at 1046.693 kb on + strand, within Pf1N1B4_921 at 1046.954 kb on + strand, within Pf1N1B4_921
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,043,780 + +0.3 1,043,781 - +1.0 1,043,794 - +0.8 1,043,794 - +0.0 1,043,794 - +2.9 1,043,817 + +1.4 1,043,818 - -0.5 1,043,818 - -1.3 1,043,818 - +0.3 1,043,818 - -1.0 1,043,993 - Pf1N1B4_918 0.22 -0.5 1,044,051 + Pf1N1B4_918 0.31 -1.1 1,044,058 + Pf1N1B4_918 0.32 +0.0 1,044,059 - Pf1N1B4_918 0.32 +0.8 1,044,059 - Pf1N1B4_918 0.32 +0.6 1,044,059 - Pf1N1B4_918 0.32 +1.2 1,044,059 - Pf1N1B4_918 0.32 +0.9 1,044,158 - Pf1N1B4_918 0.47 +0.0 1,044,158 - Pf1N1B4_918 0.47 +0.3 1,044,208 + Pf1N1B4_918 0.54 -0.8 1,044,209 - Pf1N1B4_918 0.55 -0.5 1,044,209 - Pf1N1B4_918 0.55 +0.8 1,044,253 + Pf1N1B4_918 0.61 +0.0 1,044,253 + Pf1N1B4_918 0.61 -0.2 1,044,254 - Pf1N1B4_918 0.61 +1.5 1,044,254 - Pf1N1B4_918 0.61 +1.0 1,044,254 - Pf1N1B4_918 0.61 -1.1 1,044,254 - Pf1N1B4_918 0.61 +1.4 1,044,270 + Pf1N1B4_918 0.64 +1.5 1,044,270 + Pf1N1B4_918 0.64 -2.7 1,044,271 - Pf1N1B4_918 0.64 +0.1 1,044,271 - Pf1N1B4_918 0.64 -0.3 1,044,348 + +1.0 1,044,348 + Pf1N1B4_918 0.76 -1.0 1,044,349 - Pf1N1B4_918 0.76 -0.1 1,044,394 - Pf1N1B4_918 0.83 -2.4 1,044,397 + Pf1N1B4_918 0.83 -1.1 1,044,397 + Pf1N1B4_918 0.83 -0.7 1,044,397 + Pf1N1B4_918 0.83 -0.3 1,044,397 + Pf1N1B4_918 0.83 -0.7 1,044,398 - Pf1N1B4_918 0.83 +0.5 1,044,398 - Pf1N1B4_918 0.83 +1.5 1,044,398 - Pf1N1B4_918 0.83 -1.8 1,044,398 - Pf1N1B4_918 0.83 +0.5 1,044,398 - Pf1N1B4_918 0.83 -1.0 1,044,398 - Pf1N1B4_918 0.83 +0.2 1,044,398 - Pf1N1B4_918 0.83 -1.3 1,044,398 - Pf1N1B4_918 0.83 -1.0 1,044,398 - Pf1N1B4_918 0.83 -1.0 1,044,398 - Pf1N1B4_918 0.83 -0.5 1,044,398 - Pf1N1B4_918 0.83 +1.0 1,044,398 - +0.0 1,044,398 - Pf1N1B4_918 0.83 +0.0 1,044,512 - +3.4 1,044,512 - +0.7 1,044,532 - +0.8 1,044,532 - +1.4 1,044,554 + +0.6 1,044,554 + -0.1 1,044,554 + +0.9 1,044,555 - -0.1 1,044,555 - +0.7 1,044,555 - -1.7 1,044,555 - +1.0 1,044,555 - +1.3 1,044,555 - -0.5 1,044,568 + +1.6 1,044,569 - -0.5 1,044,569 - -0.2 1,044,569 - +2.0 1,044,569 - -0.5 1,044,570 + +0.0 1,044,571 - +0.5 1,044,571 - -0.0 1,044,571 - +1.9 1,044,571 - +0.5 1,044,571 - +2.3 1,044,571 - +3.6 1,044,628 - Pf1N1B4_919 0.40 +1.9 1,044,628 - Pf1N1B4_919 0.40 +0.4 1,044,675 + Pf1N1B4_919 0.65 +0.5 1,044,675 + Pf1N1B4_919 0.65 +0.6 1,044,974 - Pf1N1B4_920 0.17 -0.1 1,045,123 + Pf1N1B4_920 0.28 +1.6 1,045,123 + Pf1N1B4_920 0.28 +1.3 1,045,123 + Pf1N1B4_920 0.28 +1.4 1,045,124 - Pf1N1B4_920 0.28 +0.4 1,045,124 - Pf1N1B4_920 0.28 +1.2 1,045,124 - Pf1N1B4_920 0.28 -0.3 1,045,210 + Pf1N1B4_920 0.35 -0.1 1,045,210 + Pf1N1B4_920 0.35 +0.6 1,045,210 + Pf1N1B4_920 0.35 +0.5 1,045,210 + Pf1N1B4_920 0.35 -1.5 1,045,210 + Pf1N1B4_920 0.35 +1.4 1,045,210 + Pf1N1B4_920 0.35 -0.2 1,045,210 + +1.6 1,045,211 - Pf1N1B4_920 0.35 +0.9 1,045,211 - Pf1N1B4_920 0.35 +1.8 1,045,211 - Pf1N1B4_920 0.35 +1.6 1,045,211 - Pf1N1B4_920 0.35 +3.4 1,045,591 + Pf1N1B4_920 0.63 -1.5 1,045,591 + Pf1N1B4_920 0.63 +1.3 1,045,591 + Pf1N1B4_920 0.63 +0.9 1,045,591 + Pf1N1B4_920 0.63 -0.7 1,045,591 + Pf1N1B4_920 0.63 -0.8 1,045,591 + Pf1N1B4_920 0.63 +0.8 1,045,591 + -0.5 1,045,591 + Pf1N1B4_920 0.63 +1.7 1,045,591 + Pf1N1B4_920 0.63 +2.0 1,045,592 - Pf1N1B4_920 0.63 +1.6 1,045,592 - Pf1N1B4_920 0.63 +0.2 1,045,592 - Pf1N1B4_920 0.63 +0.3 1,045,592 - Pf1N1B4_920 0.63 +0.1 1,045,615 + Pf1N1B4_920 0.65 +0.9 1,045,615 + Pf1N1B4_920 0.65 +1.0 1,045,615 + Pf1N1B4_920 0.65 +0.9 1,045,615 + Pf1N1B4_920 0.65 -1.8 1,045,616 - Pf1N1B4_920 0.65 +1.0 1,045,616 - Pf1N1B4_920 0.65 +1.0 1,045,616 - Pf1N1B4_920 0.65 +1.0 1,045,616 - Pf1N1B4_920 0.65 +2.0 1,045,627 + Pf1N1B4_920 0.66 +2.6 1,045,627 + Pf1N1B4_920 0.66 +1.0 1,045,628 - Pf1N1B4_920 0.66 +2.3 1,045,715 + Pf1N1B4_920 0.73 -0.8 1,045,715 + Pf1N1B4_920 0.73 +0.7 1,045,716 - Pf1N1B4_920 0.73 -2.0 1,045,716 - Pf1N1B4_920 0.73 +0.8 1,045,716 - Pf1N1B4_920 0.73 +0.9 1,045,716 - Pf1N1B4_920 0.73 +0.5 1,045,903 + Pf1N1B4_920 0.87 -0.4 1,045,903 + Pf1N1B4_920 0.87 +2.0 1,045,903 + Pf1N1B4_920 0.87 -1.3 1,045,903 + Pf1N1B4_920 0.87 +0.8 1,045,904 - Pf1N1B4_920 0.87 +0.7 1,045,904 - Pf1N1B4_920 0.87 +1.0 1,045,904 - Pf1N1B4_920 0.87 +1.4 1,045,982 - +1.0 1,046,191 - +1.0 1,046,234 + -1.5 1,046,234 + +0.9 1,046,235 - -0.7 1,046,235 - +1.2 1,046,235 - +0.5 1,046,235 - -0.5 1,046,235 - -1.9 1,046,390 + Pf1N1B4_921 0.18 +0.2 1,046,390 + Pf1N1B4_921 0.18 +0.6 1,046,390 + Pf1N1B4_921 0.18 -2.4 1,046,390 + Pf1N1B4_921 0.18 -2.0 1,046,391 - Pf1N1B4_921 0.18 -1.8 1,046,391 - Pf1N1B4_921 0.18 +1.8 1,046,391 - Pf1N1B4_921 0.18 -0.5 1,046,391 - Pf1N1B4_921 0.18 +1.2 1,046,420 + Pf1N1B4_921 0.21 +1.0 1,046,421 - Pf1N1B4_921 0.21 -1.2 1,046,421 - Pf1N1B4_921 0.21 -0.2 1,046,421 - Pf1N1B4_921 0.21 +0.5 1,046,421 - Pf1N1B4_921 0.21 +0.3 1,046,421 - Pf1N1B4_921 0.21 -0.4 1,046,441 + Pf1N1B4_921 0.23 -1.0 1,046,441 + Pf1N1B4_921 0.23 +0.3 1,046,442 - Pf1N1B4_921 0.24 +0.4 1,046,442 - +0.0 1,046,655 - Pf1N1B4_921 0.46 -1.3 1,046,655 - Pf1N1B4_921 0.46 +0.6 1,046,655 - Pf1N1B4_921 0.46 +1.0 1,046,655 - Pf1N1B4_921 0.46 +1.5 1,046,693 + Pf1N1B4_921 0.50 +0.3 1,046,954 + Pf1N1B4_921 0.78 +1.6
Or see this region's nucleotide sequence