Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_867 and Pf1N1B4_868 are separated by 2 nucleotides Pf1N1B4_868 and Pf1N1B4_869 are separated by 146 nucleotides
Pf1N1B4_867 - Serine acetyltransferase (EC 2.3.1.30), at 994,141 to 994,917
_867
Pf1N1B4_868 - tRNA:Cm32/Um32 methyltransferase, at 994,920 to 995,690
_868
Pf1N1B4_869 - Inositol-1-monophosphatase (EC 3.1.3.25), at 995,837 to 996,655
_869
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 993.990 kb on - strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.142 kb on + strand at 994.171 kb on + strand at 994.172 kb on - strand at 994.220 kb on + strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.273 kb on + strand, within Pf1N1B4_867 at 994.936 kb on - strand at 994.936 kb on - strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.988 kb on + strand at 994.988 kb on + strand at 994.989 kb on - strand at 995.024 kb on - strand, within Pf1N1B4_868 at 995.110 kb on + strand, within Pf1N1B4_868 at 995.111 kb on - strand at 995.111 kb on - strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.299 kb on + strand, within Pf1N1B4_868 at 995.300 kb on - strand, within Pf1N1B4_868 at 995.388 kb on + strand, within Pf1N1B4_868 at 995.389 kb on - strand, within Pf1N1B4_868 at 995.730 kb on + strand at 995.742 kb on + strand at 995.742 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 993,990 - -0.8 994,007 + -2.4 994,007 + +0.3 994,007 + +2.9 994,142 + +0.0 994,171 + -1.0 994,172 - -0.2 994,220 + Pf1N1B4_867 0.10 -1.5 994,221 - Pf1N1B4_867 0.10 +0.4 994,221 - Pf1N1B4_867 0.10 -1.5 994,221 - Pf1N1B4_867 0.10 -0.1 994,221 - Pf1N1B4_867 0.10 +0.7 994,273 + Pf1N1B4_867 0.17 +0.7 994,936 - +2.1 994,936 - -1.5 994,986 + +1.1 994,986 + +1.1 994,986 + +0.4 994,987 - -0.1 994,987 - -1.3 994,987 - -1.9 994,987 - +1.1 994,987 - -0.5 994,987 - +0.9 994,987 - +1.1 994,987 - +1.1 994,987 - -0.2 994,987 - -0.2 994,987 - +0.7 994,987 - -0.5 994,987 - +0.4 994,988 + +2.4 994,988 + -0.5 994,989 - -0.5 995,024 - Pf1N1B4_868 0.13 -0.3 995,110 + Pf1N1B4_868 0.25 +1.3 995,111 - +1.6 995,111 - Pf1N1B4_868 0.25 +2.2 995,216 + Pf1N1B4_868 0.38 +1.6 995,216 + Pf1N1B4_868 0.38 -0.5 995,216 + Pf1N1B4_868 0.38 -1.8 995,216 + Pf1N1B4_868 0.38 +1.8 995,217 - Pf1N1B4_868 0.39 +1.1 995,217 - Pf1N1B4_868 0.39 +1.1 995,217 - Pf1N1B4_868 0.39 +1.6 995,217 - Pf1N1B4_868 0.39 +1.2 995,217 - Pf1N1B4_868 0.39 +0.6 995,217 - Pf1N1B4_868 0.39 +0.1 995,217 - Pf1N1B4_868 0.39 +0.1 995,227 + Pf1N1B4_868 0.40 +1.6 995,227 + Pf1N1B4_868 0.40 +1.1 995,227 + Pf1N1B4_868 0.40 +1.6 995,227 + Pf1N1B4_868 0.40 +1.4 995,227 + Pf1N1B4_868 0.40 +1.8 995,227 + Pf1N1B4_868 0.40 +2.3 995,228 - Pf1N1B4_868 0.40 -0.4 995,228 - Pf1N1B4_868 0.40 +0.1 995,228 - Pf1N1B4_868 0.40 +0.3 995,228 - Pf1N1B4_868 0.40 +0.9 995,228 - Pf1N1B4_868 0.40 +0.8 995,299 + Pf1N1B4_868 0.49 +1.3 995,300 - Pf1N1B4_868 0.49 -0.1 995,388 + Pf1N1B4_868 0.61 +1.2 995,389 - Pf1N1B4_868 0.61 +0.6 995,730 + +1.8 995,742 + +1.1 995,742 + +1.4 995,753 + +0.5 995,753 + +0.9 995,753 + -0.4
Or see this region's nucleotide sequence