Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_760 and Pf1N1B4_761 are separated by 134 nucleotides Pf1N1B4_761 and Pf1N1B4_762 are separated by 403 nucleotides Pf1N1B4_762 and Pf1N1B4_763 overlap by 4 nucleotides
Pf1N1B4_760 - Formate dehydrogenase-O, major subunit (EC 1.2.1.2), at 863,239 to 865,344
_760
Pf1N1B4_761 - FIG00954879: hypothetical protein, at 865,479 to 865,769
_761
Pf1N1B4_762 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 866,173 to 867,096
_762
Pf1N1B4_763 - Ferric iron ABC transporter, ATP-binding protein, at 867,093 to 868,202
_763
Position (kb)
866
867
868 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 865.255 kb on + strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.727 kb on + strand at 865.728 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.747 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.804 kb on + strand at 865.804 kb on + strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.941 kb on + strand at 865.941 kb on + strand at 865.941 kb on + strand at 865.942 kb on - strand at 865.951 kb on + strand at 866.052 kb on + strand at 866.098 kb on + strand at 866.128 kb on - strand at 866.143 kb on + strand at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.674 kb on + strand, within Pf1N1B4_762 at 866.675 kb on - strand, within Pf1N1B4_762 at 866.675 kb on - strand, within Pf1N1B4_762 at 866.676 kb on + strand, within Pf1N1B4_762 at 866.676 kb on + strand, within Pf1N1B4_762 at 866.676 kb on + strand, within Pf1N1B4_762 at 866.677 kb on - strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.678 kb on + strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.679 kb on - strand, within Pf1N1B4_762 at 866.714 kb on + strand, within Pf1N1B4_762 at 867.628 kb on + strand, within Pf1N1B4_763 at 867.628 kb on + strand, within Pf1N1B4_763 at 867.629 kb on - strand at 867.629 kb on - strand, within Pf1N1B4_763 at 867.629 kb on - strand, within Pf1N1B4_763 at 867.757 kb on - strand, within Pf1N1B4_763 at 867.765 kb on + strand, within Pf1N1B4_763 at 867.814 kb on - strand, within Pf1N1B4_763 at 867.832 kb on + strand, within Pf1N1B4_763 at 867.833 kb on - strand, within Pf1N1B4_763 at 867.872 kb on - strand, within Pf1N1B4_763 at 867.872 kb on - strand, within Pf1N1B4_763 at 867.888 kb on + strand, within Pf1N1B4_763 at 867.888 kb on + strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 868.090 kb on + strand, within Pf1N1B4_763
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 865,255 + -0.5 865,398 - +0.8 865,398 - -0.2 865,398 - +0.8 865,398 - -1.3 865,727 + -1.6 865,728 - Pf1N1B4_761 0.86 -0.6 865,738 - Pf1N1B4_761 0.89 -1.8 865,738 - Pf1N1B4_761 0.89 +1.0 865,738 - Pf1N1B4_761 0.89 +0.1 865,738 - Pf1N1B4_761 0.89 +0.5 865,747 - +1.0 865,801 - -1.3 865,801 - +0.1 865,801 - -1.2 865,801 - -0.2 865,801 - +0.6 865,801 - -1.6 865,804 + -0.8 865,804 + -1.8 865,805 - +0.3 865,805 - +0.0 865,805 - +0.8 865,805 - -0.2 865,941 + +0.0 865,941 + -0.1 865,941 + +1.5 865,942 - -0.8 865,951 + -0.3 866,052 + +2.0 866,098 + +0.4 866,128 - -1.3 866,143 + +0.0 866,674 + Pf1N1B4_762 0.54 -1.8 866,674 + Pf1N1B4_762 0.54 +0.0 866,674 + Pf1N1B4_762 0.54 -2.4 866,674 + Pf1N1B4_762 0.54 -0.3 866,674 + Pf1N1B4_762 0.54 -0.3 866,674 + Pf1N1B4_762 0.54 +1.0 866,674 + Pf1N1B4_762 0.54 +0.3 866,674 + Pf1N1B4_762 0.54 +0.0 866,675 - Pf1N1B4_762 0.54 -1.3 866,675 - Pf1N1B4_762 0.54 +0.2 866,676 + Pf1N1B4_762 0.54 +0.4 866,676 + Pf1N1B4_762 0.54 -1.0 866,676 + Pf1N1B4_762 0.54 -1.5 866,677 - Pf1N1B4_762 0.55 +0.0 866,678 + Pf1N1B4_762 0.55 +0.0 866,678 + Pf1N1B4_762 0.55 +1.0 866,678 + Pf1N1B4_762 0.55 -2.0 866,678 + Pf1N1B4_762 0.55 -1.4 866,678 + +1.0 866,678 + Pf1N1B4_762 0.55 -2.0 866,678 + Pf1N1B4_762 0.55 +0.2 866,678 + Pf1N1B4_762 0.55 +3.1 866,678 + Pf1N1B4_762 0.55 +1.0 866,678 + Pf1N1B4_762 0.55 +0.4 866,678 + Pf1N1B4_762 0.55 -1.6 866,679 - Pf1N1B4_762 0.55 -0.6 866,679 - Pf1N1B4_762 0.55 -1.3 866,679 - Pf1N1B4_762 0.55 -1.3 866,679 - Pf1N1B4_762 0.55 -1.3 866,679 - Pf1N1B4_762 0.55 +2.0 866,679 - Pf1N1B4_762 0.55 -1.6 866,679 - Pf1N1B4_762 0.55 +0.0 866,679 - Pf1N1B4_762 0.55 +0.0 866,679 - Pf1N1B4_762 0.55 -0.3 866,679 - Pf1N1B4_762 0.55 +0.4 866,679 - Pf1N1B4_762 0.55 +0.4 866,714 + Pf1N1B4_762 0.59 -1.0 867,628 + Pf1N1B4_763 0.48 +0.4 867,628 + Pf1N1B4_763 0.48 +0.6 867,629 - +1.0 867,629 - Pf1N1B4_763 0.48 -0.4 867,629 - Pf1N1B4_763 0.48 +1.0 867,757 - Pf1N1B4_763 0.60 -1.3 867,765 + Pf1N1B4_763 0.61 +0.6 867,814 - Pf1N1B4_763 0.65 -1.6 867,832 + Pf1N1B4_763 0.67 -3.0 867,833 - Pf1N1B4_763 0.67 +1.0 867,872 - Pf1N1B4_763 0.70 +1.4 867,872 - Pf1N1B4_763 0.70 +0.0 867,888 + Pf1N1B4_763 0.72 +0.0 867,888 + Pf1N1B4_763 0.72 +0.0 867,889 - Pf1N1B4_763 0.72 -0.3 867,889 - Pf1N1B4_763 0.72 -1.0 867,889 - Pf1N1B4_763 0.72 -0.6 868,090 + Pf1N1B4_763 0.90 -0.4
Or see this region's nucleotide sequence