Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_72 and Pf1N1B4_73 are separated by 142 nucleotides Pf1N1B4_73 and Pf1N1B4_74 are separated by 231 nucleotides Pf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotides
Pf1N1B4_72 - Transcriptional regulator, LysR family, at 75,747 to 76,619
_72
Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934
_73
Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429
_74
Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823
_75
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 75.829 kb on + strand at 76.251 kb on + strand, within Pf1N1B4_72 at 76.251 kb on + strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.656 kb on + strand at 76.657 kb on - strand at 76.688 kb on + strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.786 kb on - strand at 76.813 kb on + strand at 76.814 kb on - strand at 76.973 kb on - strand, within Pf1N1B4_73 at 76.973 kb on - strand, within Pf1N1B4_73 at 77.071 kb on + strand, within Pf1N1B4_73 at 77.124 kb on - strand, within Pf1N1B4_73 at 77.132 kb on + strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.245 kb on + strand, within Pf1N1B4_73 at 77.246 kb on - strand, within Pf1N1B4_73 at 77.293 kb on + strand, within Pf1N1B4_73 at 77.407 kb on + strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.440 kb on + strand, within Pf1N1B4_73 at 77.452 kb on + strand, within Pf1N1B4_73 at 77.620 kb on + strand at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.672 kb on - strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.689 kb on - strand, within Pf1N1B4_73 at 77.689 kb on - strand, within Pf1N1B4_73 at 77.819 kb on - strand at 77.876 kb on - strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.913 kb on - strand at 77.913 kb on - strand at 78.032 kb on + strand at 78.033 kb on - strand at 78.033 kb on - strand at 78.033 kb on - strand at 78.040 kb on - strand at 78.060 kb on - strand at 78.121 kb on + strand at 78.122 kb on - strand at 78.139 kb on - strand at 78.139 kb on - strand at 78.167 kb on + strand at 78.168 kb on - strand at 78.168 kb on - strand at 78.205 kb on + strand, within Pf1N1B4_74 at 78.206 kb on - strand, within Pf1N1B4_74 at 78.206 kb on - strand, within Pf1N1B4_74 at 78.304 kb on + strand, within Pf1N1B4_74 at 78.305 kb on - strand, within Pf1N1B4_74 at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.685 kb on + strand, within Pf1N1B4_75 at 78.685 kb on + strand, within Pf1N1B4_75 at 78.686 kb on - strand, within Pf1N1B4_75 at 78.813 kb on - strand at 78.844 kb on + strand at 78.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 75,829 + -1.2 76,251 + Pf1N1B4_72 0.58 +0.2 76,251 + Pf1N1B4_72 0.58 +1.8 76,252 - Pf1N1B4_72 0.58 +0.8 76,252 - Pf1N1B4_72 0.58 +0.1 76,656 + +4.8 76,657 - -1.2 76,688 + +4.4 76,689 - +0.8 76,689 - -0.2 76,689 - -1.6 76,689 - -0.2 76,786 - -2.5 76,813 + -2.1 76,814 - -1.6 76,973 - Pf1N1B4_73 0.18 -2.8 76,973 - Pf1N1B4_73 0.18 +0.1 77,071 + Pf1N1B4_73 0.26 -2.6 77,124 - Pf1N1B4_73 0.31 +0.3 77,132 + Pf1N1B4_73 0.32 -1.5 77,133 - Pf1N1B4_73 0.32 -0.9 77,133 - Pf1N1B4_73 0.32 -3.1 77,133 - Pf1N1B4_73 0.32 -0.2 77,133 - Pf1N1B4_73 0.32 +0.8 77,179 + Pf1N1B4_73 0.36 -1.2 77,179 + Pf1N1B4_73 0.36 -0.8 77,179 + Pf1N1B4_73 0.36 -1.8 77,180 - Pf1N1B4_73 0.36 -0.8 77,180 - Pf1N1B4_73 0.36 -1.5 77,245 + Pf1N1B4_73 0.41 -2.0 77,246 - Pf1N1B4_73 0.41 -3.1 77,293 + Pf1N1B4_73 0.45 -0.5 77,407 + Pf1N1B4_73 0.55 -0.5 77,408 - Pf1N1B4_73 0.55 -2.3 77,408 - Pf1N1B4_73 0.55 -2.2 77,408 - Pf1N1B4_73 0.55 +0.0 77,440 + Pf1N1B4_73 0.58 -1.5 77,452 + Pf1N1B4_73 0.59 -1.5 77,620 + -1.5 77,623 + Pf1N1B4_73 0.73 -2.2 77,623 + Pf1N1B4_73 0.73 -1.8 77,623 + Pf1N1B4_73 0.73 -0.8 77,623 + Pf1N1B4_73 0.73 -1.8 77,623 + Pf1N1B4_73 0.73 -0.8 77,623 + Pf1N1B4_73 0.73 -0.8 77,624 - Pf1N1B4_73 0.73 +0.6 77,624 - Pf1N1B4_73 0.73 -1.5 77,624 - Pf1N1B4_73 0.73 -0.2 77,624 - Pf1N1B4_73 0.73 -0.8 77,665 + Pf1N1B4_73 0.77 +0.8 77,665 + Pf1N1B4_73 0.77 -1.2 77,665 + Pf1N1B4_73 0.77 -1.2 77,665 + Pf1N1B4_73 0.77 -1.3 77,665 + Pf1N1B4_73 0.77 -1.0 77,665 + Pf1N1B4_73 0.77 -1.4 77,666 - Pf1N1B4_73 0.77 -1.0 77,666 - Pf1N1B4_73 0.77 -2.3 77,666 - Pf1N1B4_73 0.77 +0.4 77,666 - Pf1N1B4_73 0.77 -1.5 77,672 - Pf1N1B4_73 0.78 +0.8 77,688 + Pf1N1B4_73 0.79 -0.8 77,688 + Pf1N1B4_73 0.79 +0.2 77,688 + Pf1N1B4_73 0.79 +0.2 77,689 - Pf1N1B4_73 0.79 -1.2 77,689 - Pf1N1B4_73 0.79 -3.1 77,819 - -2.2 77,876 - -0.5 77,912 + -0.5 77,912 + -1.8 77,912 + -0.9 77,912 + -2.7 77,913 - -2.3 77,913 - +0.8 78,032 + +0.6 78,033 - -1.8 78,033 - -1.4 78,033 - -1.8 78,040 - -2.0 78,060 - -0.2 78,121 + +0.5 78,122 - -2.3 78,139 - +0.2 78,139 - -2.2 78,167 + +0.6 78,168 - -1.2 78,168 - -0.1 78,205 + Pf1N1B4_74 0.15 -0.0 78,206 - Pf1N1B4_74 0.15 +0.8 78,206 - Pf1N1B4_74 0.15 -1.0 78,304 + Pf1N1B4_74 0.52 -0.2 78,305 - Pf1N1B4_74 0.53 -0.2 78,419 + +0.6 78,419 + -0.6 78,419 + -1.2 78,419 + +2.4 78,420 - -0.4 78,420 - +1.4 78,420 - +0.2 78,420 - +0.8 78,420 - -0.5 78,685 + Pf1N1B4_75 0.63 -0.8 78,685 + Pf1N1B4_75 0.63 +0.2 78,686 - Pf1N1B4_75 0.63 -2.3 78,813 - +0.8 78,844 + -1.8 78,888 + +0.5
Or see this region's nucleotide sequence