Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_707

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_706 and Pf1N1B4_707 are separated by 116 nucleotidesPf1N1B4_707 and Pf1N1B4_708 are separated by 96 nucleotides Pf1N1B4_706 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 805,954 to 806,820 _706 Pf1N1B4_707 - Nucleoside-diphosphate-sugar epimerases, at 806,937 to 807,929 _707 Pf1N1B4_708 - Chromosome initiation inhibitor, at 808,026 to 808,925 _708 Position (kb) 806 807 808Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 806.867 kb on + strandat 806.867 kb on + strandat 806.867 kb on + strandat 806.867 kb on + strandat 806.867 kb on + strandat 806.867 kb on + strandat 806.867 kb on + strandat 806.868 kb on - strandat 806.868 kb on - strandat 806.868 kb on - strandat 806.868 kb on - strandat 806.868 kb on - strandat 806.868 kb on - strandat 807.011 kb on - strandat 807.011 kb on - strandat 807.011 kb on - strandat 807.011 kb on - strandat 807.019 kb on + strandat 807.208 kb on + strand, within Pf1N1B4_707at 807.208 kb on + strand, within Pf1N1B4_707at 807.208 kb on + strand, within Pf1N1B4_707at 807.208 kb on + strandat 807.208 kb on + strand, within Pf1N1B4_707at 807.208 kb on + strand, within Pf1N1B4_707at 807.208 kb on + strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.209 kb on - strand, within Pf1N1B4_707at 807.256 kb on + strand, within Pf1N1B4_707at 807.256 kb on + strand, within Pf1N1B4_707at 807.257 kb on - strand, within Pf1N1B4_707at 807.502 kb on + strand, within Pf1N1B4_707at 807.503 kb on - strand, within Pf1N1B4_707at 807.503 kb on - strand, within Pf1N1B4_707at 807.503 kb on - strandat 807.583 kb on + strand, within Pf1N1B4_707at 807.584 kb on - strand, within Pf1N1B4_707at 807.584 kb on - strand, within Pf1N1B4_707at 807.941 kb on - strandat 807.941 kb on - strandat 807.941 kb on - strandat 807.952 kb on + strandat 807.953 kb on - strandat 807.967 kb on - strandat 807.981 kb on - strandat 808.148 kb on + strandat 808.148 kb on + strand, within Pf1N1B4_708at 808.148 kb on + strand, within Pf1N1B4_708at 808.148 kb on + strand, within Pf1N1B4_708at 808.149 kb on - strand, within Pf1N1B4_708at 808.269 kb on + strand, within Pf1N1B4_708at 808.269 kb on + strand, within Pf1N1B4_708at 808.269 kb on + strandat 808.269 kb on + strand, within Pf1N1B4_708at 808.269 kb on + strand, within Pf1N1B4_708at 808.269 kb on + strand, within Pf1N1B4_708at 808.269 kb on + strand, within Pf1N1B4_708at 808.270 kb on - strand, within Pf1N1B4_708at 808.270 kb on - strandat 808.270 kb on - strand, within Pf1N1B4_708at 808.270 kb on - strandat 808.270 kb on - strand, within Pf1N1B4_708at 808.270 kb on - strand, within Pf1N1B4_708at 808.324 kb on - strand, within Pf1N1B4_708at 808.549 kb on - strand, within Pf1N1B4_708at 808.908 kb on + strandat 808.908 kb on + strandat 808.909 kb on - strandat 808.909 kb on - strandat 808.916 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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806,867 + +1.3
806,867 + -0.6
806,867 + -0.7
806,867 + +0.1
806,867 + +2.0
806,867 + -1.6
806,867 + +0.0
806,868 - -0.6
806,868 - -0.6
806,868 - +2.6
806,868 - -1.0
806,868 - +1.7
806,868 - +1.5
807,011 - +0.7
807,011 - +1.0
807,011 - -1.3
807,011 - -1.3
807,019 + -0.6
807,208 + Pf1N1B4_707 0.27 +2.3
807,208 + Pf1N1B4_707 0.27 -1.3
807,208 + Pf1N1B4_707 0.27 -0.3
807,208 + -0.6
807,208 + Pf1N1B4_707 0.27 -1.2
807,208 + Pf1N1B4_707 0.27 +1.0
807,208 + Pf1N1B4_707 0.27 -0.6
807,209 - Pf1N1B4_707 0.27 -0.6
807,209 - Pf1N1B4_707 0.27 -0.6
807,209 - Pf1N1B4_707 0.27 +0.0
807,209 - Pf1N1B4_707 0.27 -1.3
807,209 - Pf1N1B4_707 0.27 -2.0
807,209 - Pf1N1B4_707 0.27 +0.0
807,209 - Pf1N1B4_707 0.27 -1.6
807,209 - Pf1N1B4_707 0.27 -0.2
807,256 + Pf1N1B4_707 0.32 -0.6
807,256 + Pf1N1B4_707 0.32 -0.8
807,257 - Pf1N1B4_707 0.32 -0.7
807,502 + Pf1N1B4_707 0.57 -0.6
807,503 - Pf1N1B4_707 0.57 +0.0
807,503 - Pf1N1B4_707 0.57 -0.9
807,503 - +0.0
807,583 + Pf1N1B4_707 0.65 -1.5
807,584 - Pf1N1B4_707 0.65 -0.6
807,584 - Pf1N1B4_707 0.65 -1.3
807,941 - -0.6
807,941 - -0.2
807,941 - -0.0
807,952 + +0.0
807,953 - -0.2
807,967 - +0.4
807,981 - +0.0
808,148 + +1.0
808,148 + Pf1N1B4_708 0.14 +0.4
808,148 + Pf1N1B4_708 0.14 -0.9
808,148 + Pf1N1B4_708 0.14 -1.3
808,149 - Pf1N1B4_708 0.14 -0.8
808,269 + Pf1N1B4_708 0.27 +1.0
808,269 + Pf1N1B4_708 0.27 -0.3
808,269 + +1.0
808,269 + Pf1N1B4_708 0.27 +0.0
808,269 + Pf1N1B4_708 0.27 -1.6
808,269 + Pf1N1B4_708 0.27 +1.5
808,269 + Pf1N1B4_708 0.27 -0.6
808,270 - Pf1N1B4_708 0.27 +0.6
808,270 - +1.0
808,270 - Pf1N1B4_708 0.27 +0.5
808,270 - -1.0
808,270 - Pf1N1B4_708 0.27 -0.0
808,270 - Pf1N1B4_708 0.27 -1.3
808,324 - Pf1N1B4_708 0.33 -2.9
808,549 - Pf1N1B4_708 0.58 -1.6
808,908 + +1.0
808,908 + +1.0
808,909 - +0.0
808,909 - -0.8
808,916 - -2.9

Or see this region's nucleotide sequence