Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_706 and Pf1N1B4_707 are separated by 116 nucleotides Pf1N1B4_707 and Pf1N1B4_708 are separated by 96 nucleotides
Pf1N1B4_706 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 805,954 to 806,820
_706
Pf1N1B4_707 - Nucleoside-diphosphate-sugar epimerases, at 806,937 to 807,929
_707
Pf1N1B4_708 - Chromosome initiation inhibitor, at 808,026 to 808,925
_708
Position (kb)
806
807
808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 806.867 kb on + strand at 806.867 kb on + strand at 806.867 kb on + strand at 806.867 kb on + strand at 806.867 kb on + strand at 806.867 kb on + strand at 806.867 kb on + strand at 806.868 kb on - strand at 806.868 kb on - strand at 806.868 kb on - strand at 806.868 kb on - strand at 806.868 kb on - strand at 806.868 kb on - strand at 807.011 kb on - strand at 807.011 kb on - strand at 807.011 kb on - strand at 807.011 kb on - strand at 807.019 kb on + strand at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.256 kb on + strand, within Pf1N1B4_707 at 807.256 kb on + strand, within Pf1N1B4_707 at 807.257 kb on - strand, within Pf1N1B4_707 at 807.502 kb on + strand, within Pf1N1B4_707 at 807.503 kb on - strand, within Pf1N1B4_707 at 807.503 kb on - strand, within Pf1N1B4_707 at 807.503 kb on - strand at 807.583 kb on + strand, within Pf1N1B4_707 at 807.584 kb on - strand, within Pf1N1B4_707 at 807.584 kb on - strand, within Pf1N1B4_707 at 807.941 kb on - strand at 807.941 kb on - strand at 807.941 kb on - strand at 807.952 kb on + strand at 807.953 kb on - strand at 807.967 kb on - strand at 807.981 kb on - strand at 808.148 kb on + strand at 808.148 kb on + strand, within Pf1N1B4_708 at 808.148 kb on + strand, within Pf1N1B4_708 at 808.148 kb on + strand, within Pf1N1B4_708 at 808.149 kb on - strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand, within Pf1N1B4_708 at 808.324 kb on - strand, within Pf1N1B4_708 at 808.549 kb on - strand, within Pf1N1B4_708 at 808.908 kb on + strand at 808.908 kb on + strand at 808.909 kb on - strand at 808.909 kb on - strand at 808.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 806,867 + +1.3 806,867 + -0.6 806,867 + -0.7 806,867 + +0.1 806,867 + +2.0 806,867 + -1.6 806,867 + +0.0 806,868 - -0.6 806,868 - -0.6 806,868 - +2.6 806,868 - -1.0 806,868 - +1.7 806,868 - +1.5 807,011 - +0.7 807,011 - +1.0 807,011 - -1.3 807,011 - -1.3 807,019 + -0.6 807,208 + Pf1N1B4_707 0.27 +2.3 807,208 + Pf1N1B4_707 0.27 -1.3 807,208 + Pf1N1B4_707 0.27 -0.3 807,208 + -0.6 807,208 + Pf1N1B4_707 0.27 -1.2 807,208 + Pf1N1B4_707 0.27 +1.0 807,208 + Pf1N1B4_707 0.27 -0.6 807,209 - Pf1N1B4_707 0.27 -0.6 807,209 - Pf1N1B4_707 0.27 -0.6 807,209 - Pf1N1B4_707 0.27 +0.0 807,209 - Pf1N1B4_707 0.27 -1.3 807,209 - Pf1N1B4_707 0.27 -2.0 807,209 - Pf1N1B4_707 0.27 +0.0 807,209 - Pf1N1B4_707 0.27 -1.6 807,209 - Pf1N1B4_707 0.27 -0.2 807,256 + Pf1N1B4_707 0.32 -0.6 807,256 + Pf1N1B4_707 0.32 -0.8 807,257 - Pf1N1B4_707 0.32 -0.7 807,502 + Pf1N1B4_707 0.57 -0.6 807,503 - Pf1N1B4_707 0.57 +0.0 807,503 - Pf1N1B4_707 0.57 -0.9 807,503 - +0.0 807,583 + Pf1N1B4_707 0.65 -1.5 807,584 - Pf1N1B4_707 0.65 -0.6 807,584 - Pf1N1B4_707 0.65 -1.3 807,941 - -0.6 807,941 - -0.2 807,941 - -0.0 807,952 + +0.0 807,953 - -0.2 807,967 - +0.4 807,981 - +0.0 808,148 + +1.0 808,148 + Pf1N1B4_708 0.14 +0.4 808,148 + Pf1N1B4_708 0.14 -0.9 808,148 + Pf1N1B4_708 0.14 -1.3 808,149 - Pf1N1B4_708 0.14 -0.8 808,269 + Pf1N1B4_708 0.27 +1.0 808,269 + Pf1N1B4_708 0.27 -0.3 808,269 + +1.0 808,269 + Pf1N1B4_708 0.27 +0.0 808,269 + Pf1N1B4_708 0.27 -1.6 808,269 + Pf1N1B4_708 0.27 +1.5 808,269 + Pf1N1B4_708 0.27 -0.6 808,270 - Pf1N1B4_708 0.27 +0.6 808,270 - +1.0 808,270 - Pf1N1B4_708 0.27 +0.5 808,270 - -1.0 808,270 - Pf1N1B4_708 0.27 -0.0 808,270 - Pf1N1B4_708 0.27 -1.3 808,324 - Pf1N1B4_708 0.33 -2.9 808,549 - Pf1N1B4_708 0.58 -1.6 808,908 + +1.0 808,908 + +1.0 808,909 - +0.0 808,909 - -0.8 808,916 - -2.9
Or see this region's nucleotide sequence