Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_676 and Pf1N1B4_677 are separated by 222 nucleotides Pf1N1B4_677 and Pf1N1B4_678 are separated by 257 nucleotides Pf1N1B4_678 and Pf1N1B4_679 are separated by 167 nucleotides
Pf1N1B4_676 - FIG00953991: hypothetical protein, at 773,494 to 773,853
_676
Pf1N1B4_677 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 774,076 to 774,390
_677
Pf1N1B4_678 - Pyruvate kinase (EC 2.7.1.40), at 774,648 to 776,099
_678
Pf1N1B4_679 - Enoyl-CoA hydratase (EC 4.2.1.17), at 776,267 to 777,016
_679
Position (kb) 774
775
776
777 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 773.698 kb on - strand, within Pf1N1B4_676 at 773.761 kb on - strand, within Pf1N1B4_676 at 773.813 kb on + strand, within Pf1N1B4_676 at 773.896 kb on + strand at 773.897 kb on - strand at 773.918 kb on - strand at 773.918 kb on - strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.926 kb on - strand at 773.926 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.986 kb on + strand at 774.008 kb on + strand at 774.009 kb on - strand at 774.018 kb on + strand at 774.019 kb on - strand at 774.050 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.226 kb on - strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.365 kb on + strand at 774.365 kb on + strand at 774.365 kb on + strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.486 kb on - strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.575 kb on - strand at 774.575 kb on - strand at 774.594 kb on + strand at 774.595 kb on - strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.609 kb on + strand at 774.609 kb on + strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.678 kb on - strand at 774.695 kb on + strand at 774.696 kb on - strand at 774.696 kb on - strand at 774.696 kb on - strand at 774.932 kb on + strand, within Pf1N1B4_678 at 774.932 kb on + strand, within Pf1N1B4_678 at 774.932 kb on + strand, within Pf1N1B4_678 at 774.933 kb on - strand, within Pf1N1B4_678 at 775.046 kb on + strand, within Pf1N1B4_678 at 775.058 kb on + strand, within Pf1N1B4_678 at 775.215 kb on + strand, within Pf1N1B4_678 at 775.216 kb on - strand, within Pf1N1B4_678 at 775.216 kb on - strand, within Pf1N1B4_678 at 775.257 kb on + strand, within Pf1N1B4_678 at 775.257 kb on + strand, within Pf1N1B4_678 at 775.552 kb on + strand, within Pf1N1B4_678 at 775.552 kb on + strand, within Pf1N1B4_678 at 775.553 kb on - strand, within Pf1N1B4_678 at 775.572 kb on + strand at 775.572 kb on + strand, within Pf1N1B4_678 at 775.572 kb on + strand, within Pf1N1B4_678 at 775.572 kb on + strand, within Pf1N1B4_678 at 775.573 kb on - strand, within Pf1N1B4_678 at 775.617 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.809 kb on + strand, within Pf1N1B4_678 at 775.854 kb on + strand, within Pf1N1B4_678 at 775.854 kb on + strand at 775.855 kb on - strand, within Pf1N1B4_678 at 775.855 kb on - strand, within Pf1N1B4_678 at 775.855 kb on - strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.912 kb on - strand at 775.912 kb on - strand, within Pf1N1B4_678 at 775.918 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.038 kb on - strand at 776.281 kb on + strand at 776.282 kb on - strand at 776.282 kb on - strand at 776.665 kb on - strand, within Pf1N1B4_679 at 776.773 kb on - strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 773,698 - Pf1N1B4_676 0.57 -1.8 773,761 - Pf1N1B4_676 0.74 +0.7 773,813 + Pf1N1B4_676 0.89 +0.6 773,896 + +1.0 773,897 - +0.4 773,918 - -0.6 773,918 - +1.3 773,925 + -0.3 773,925 + -1.0 773,925 + -1.8 773,925 + +0.3 773,926 - -0.8 773,926 - -2.0 773,928 - +1.2 773,928 - +0.6 773,928 - +0.6 773,928 - -0.8 773,986 + +1.0 774,008 + +0.8 774,009 - -0.4 774,018 + -0.0 774,019 - +0.1 774,050 + +0.8 774,052 + +1.0 774,052 + +2.0 774,052 + -0.1 774,052 + -1.3 774,052 + +0.3 774,052 + +0.3 774,052 + +2.5 774,052 + +1.0 774,052 + +1.0 774,052 + -0.0 774,052 + -1.1 774,053 - +0.3 774,053 - -0.3 774,053 - -1.0 774,053 - -0.0 774,053 - -2.0 774,053 - +0.7 774,053 - -2.2 774,053 - +0.3 774,053 - +1.3 774,053 - -1.3 774,207 - Pf1N1B4_677 0.42 -0.5 774,207 - Pf1N1B4_677 0.42 -1.0 774,207 - Pf1N1B4_677 0.42 -0.6 774,226 - Pf1N1B4_677 0.48 -0.6 774,284 + Pf1N1B4_677 0.66 +3.0 774,284 + Pf1N1B4_677 0.66 +0.3 774,284 + Pf1N1B4_677 0.66 -1.3 774,284 + Pf1N1B4_677 0.66 -0.1 774,284 + Pf1N1B4_677 0.66 -1.6 774,284 + Pf1N1B4_677 0.66 +1.7 774,285 - Pf1N1B4_677 0.66 +0.2 774,285 - Pf1N1B4_677 0.66 +1.1 774,285 - Pf1N1B4_677 0.66 -2.0 774,285 - Pf1N1B4_677 0.66 -1.3 774,285 - Pf1N1B4_677 0.66 -0.5 774,285 - Pf1N1B4_677 0.66 -0.3 774,285 - Pf1N1B4_677 0.66 +0.3 774,285 - Pf1N1B4_677 0.66 +0.8 774,365 + +0.3 774,365 + -0.2 774,365 + +0.4 774,366 - +0.4 774,366 - -1.6 774,366 - -2.3 774,486 - -1.0 774,549 + +0.2 774,549 + -1.6 774,549 + -0.0 774,550 - -1.8 774,550 - -0.2 774,550 - -0.6 774,550 - +1.0 774,550 - -1.8 774,550 - -0.0 774,550 - -0.4 774,575 - -1.3 774,575 - -1.0 774,594 + -0.6 774,595 - -1.8 774,600 + +1.0 774,600 + -1.3 774,600 + -0.6 774,601 - -1.1 774,601 - -1.3 774,601 - -1.8 774,609 + -2.3 774,609 + +0.2 774,610 - -0.3 774,610 - -1.3 774,610 - -1.8 774,610 - -2.0 774,610 - -0.4 774,610 - -1.8 774,612 + -1.2 774,612 + -1.5 774,612 + -0.9 774,613 - +1.0 774,613 - +0.3 774,613 - -1.8 774,613 - -1.6 774,613 - -1.0 774,613 - +0.3 774,643 + -1.2 774,643 + -1.3 774,643 + -2.3 774,643 + -1.6 774,643 + -0.0 774,644 - +0.6 774,644 - -0.0 774,644 - -1.6 774,644 - -1.2 774,644 - -0.0 774,644 - -0.6 774,644 - -0.3 774,678 - -0.4 774,695 + -1.0 774,696 - -1.9 774,696 - -0.2 774,696 - -2.2 774,932 + Pf1N1B4_678 0.20 -0.6 774,932 + Pf1N1B4_678 0.20 -0.0 774,932 + Pf1N1B4_678 0.20 -0.6 774,933 - Pf1N1B4_678 0.20 +1.0 775,046 + Pf1N1B4_678 0.27 -0.6 775,058 + Pf1N1B4_678 0.28 +0.3 775,215 + Pf1N1B4_678 0.39 -0.6 775,216 - Pf1N1B4_678 0.39 -1.6 775,216 - Pf1N1B4_678 0.39 -2.1 775,257 + Pf1N1B4_678 0.42 -1.0 775,257 + Pf1N1B4_678 0.42 +0.8 775,552 + Pf1N1B4_678 0.62 -0.7 775,552 + Pf1N1B4_678 0.62 -3.2 775,553 - Pf1N1B4_678 0.62 -0.0 775,572 + -0.0 775,572 + Pf1N1B4_678 0.64 -1.9 775,572 + Pf1N1B4_678 0.64 -1.1 775,572 + Pf1N1B4_678 0.64 -0.6 775,573 - Pf1N1B4_678 0.64 -2.0 775,617 + Pf1N1B4_678 0.67 -1.2 775,752 + Pf1N1B4_678 0.76 -1.3 775,752 + Pf1N1B4_678 0.76 -3.3 775,752 + Pf1N1B4_678 0.76 -1.6 775,752 + Pf1N1B4_678 0.76 -1.0 775,752 + Pf1N1B4_678 0.76 -2.8 775,752 + Pf1N1B4_678 0.76 -1.0 775,752 + Pf1N1B4_678 0.76 -3.3 775,752 + Pf1N1B4_678 0.76 -1.0 775,753 - Pf1N1B4_678 0.76 -1.3 775,753 - +1.0 775,753 - Pf1N1B4_678 0.76 -0.8 775,753 - Pf1N1B4_678 0.76 +0.8 775,753 - Pf1N1B4_678 0.76 -0.6 775,753 - Pf1N1B4_678 0.76 -0.3 775,809 + Pf1N1B4_678 0.80 -0.5 775,854 + Pf1N1B4_678 0.83 -2.5 775,854 + +0.4 775,855 - Pf1N1B4_678 0.83 -2.2 775,855 - Pf1N1B4_678 0.83 -2.0 775,855 - Pf1N1B4_678 0.83 +1.0 775,911 + Pf1N1B4_678 0.87 -2.0 775,911 + Pf1N1B4_678 0.87 -1.4 775,911 + Pf1N1B4_678 0.87 -0.2 775,911 + Pf1N1B4_678 0.87 -1.0 775,912 - -1.0 775,912 - Pf1N1B4_678 0.87 -1.6 775,918 + -0.6 776,037 + -0.3 776,037 + -1.2 776,037 + -1.5 776,037 + -0.7 776,037 + -1.3 776,037 + +2.0 776,038 - -1.0 776,281 + +0.3 776,282 - +1.0 776,282 - +0.2 776,665 - Pf1N1B4_679 0.53 -0.3 776,773 - Pf1N1B4_679 0.67 +0.3 776,922 + Pf1N1B4_679 0.87 -0.4 776,922 + Pf1N1B4_679 0.87 -0.3 776,922 + Pf1N1B4_679 0.87 +0.5 776,923 - Pf1N1B4_679 0.87 +1.4 776,923 - Pf1N1B4_679 0.87 +1.0 776,923 - Pf1N1B4_679 0.87 -1.3 776,923 - Pf1N1B4_679 0.87 +2.2
Or see this region's nucleotide sequence