Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_650

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_649 and Pf1N1B4_650 are separated by 200 nucleotidesPf1N1B4_650 and Pf1N1B4_651 are separated by 139 nucleotides Pf1N1B4_649 - Small-conductance mechanosensitive channel, at 743,011 to 743,853 _649 Pf1N1B4_650 - FIG001943: hypothetical protein YajQ, at 744,054 to 744,539 _650 Pf1N1B4_651 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 744,679 to 745,596 _651 Position (kb) 744 745Strain fitness (log2 ratio) -1 0 1 2 3at 743.064 kb on + strandat 743.221 kb on + strand, within Pf1N1B4_649at 743.222 kb on - strand, within Pf1N1B4_649at 743.249 kb on - strand, within Pf1N1B4_649at 743.282 kb on + strandat 743.282 kb on + strand, within Pf1N1B4_649at 743.341 kb on - strand, within Pf1N1B4_649at 743.385 kb on + strand, within Pf1N1B4_649at 743.424 kb on + strandat 743.425 kb on - strand, within Pf1N1B4_649at 743.425 kb on - strand, within Pf1N1B4_649at 743.425 kb on - strand, within Pf1N1B4_649at 743.770 kb on + strandat 743.771 kb on - strandat 743.784 kb on - strandat 743.919 kb on + strandat 744.004 kb on + strandat 744.116 kb on + strand, within Pf1N1B4_650at 744.116 kb on + strand, within Pf1N1B4_650at 744.116 kb on + strand, within Pf1N1B4_650at 744.116 kb on + strand, within Pf1N1B4_650at 744.116 kb on + strand, within Pf1N1B4_650at 744.116 kb on + strand, within Pf1N1B4_650at 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strandat 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strand, within Pf1N1B4_650at 744.117 kb on - strandat 744.289 kb on + strand, within Pf1N1B4_650at 744.452 kb on - strand, within Pf1N1B4_650at 744.541 kb on + strandat 744.541 kb on + strandat 744.541 kb on + strandat 744.541 kb on + strandat 744.541 kb on + strandat 744.541 kb on + strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.542 kb on - strandat 744.554 kb on - strandat 744.554 kb on - strandat 744.598 kb on - strandat 744.612 kb on + strandat 744.612 kb on + strandat 744.612 kb on + strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.613 kb on - strandat 744.615 kb on - strandat 744.615 kb on - strandat 744.615 kb on - strandat 744.615 kb on - strandat 744.696 kb on + strandat 744.696 kb on + strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.791 kb on + strand, within Pf1N1B4_651at 744.792 kb on - strand, within Pf1N1B4_651at 744.792 kb on - strand, within Pf1N1B4_651at 744.792 kb on - strandat 744.850 kb on + strand, within Pf1N1B4_651at 744.850 kb on + strand, within Pf1N1B4_651at 744.903 kb on - strand, within Pf1N1B4_651at 744.903 kb on - strand, within Pf1N1B4_651at 744.919 kb on + strand, within Pf1N1B4_651at 744.919 kb on + strand, within Pf1N1B4_651at 745.114 kb on + strand, within Pf1N1B4_651at 745.115 kb on - strand, within Pf1N1B4_651at 745.182 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.439 kb on - strand, within Pf1N1B4_651at 745.439 kb on - strand, within Pf1N1B4_651at 745.477 kb on + strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.490 kb on - strand, within Pf1N1B4_651

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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743,064 + -1.0
743,221 + Pf1N1B4_649 0.25 +0.6
743,222 - Pf1N1B4_649 0.25 -0.2
743,249 - Pf1N1B4_649 0.28 -1.2
743,282 + -0.6
743,282 + Pf1N1B4_649 0.32 -0.3
743,341 - Pf1N1B4_649 0.39 -0.3
743,385 + Pf1N1B4_649 0.44 +0.4
743,424 + -0.6
743,425 - Pf1N1B4_649 0.49 +1.3
743,425 - Pf1N1B4_649 0.49 -0.6
743,425 - Pf1N1B4_649 0.49 -0.0
743,770 + -0.5
743,771 - +2.2
743,784 - -0.1
743,919 + -0.5
744,004 + +0.2
744,116 + Pf1N1B4_650 0.13 -1.0
744,116 + Pf1N1B4_650 0.13 +1.1
744,116 + Pf1N1B4_650 0.13 +1.4
744,116 + Pf1N1B4_650 0.13 +1.1
744,116 + Pf1N1B4_650 0.13 +0.6
744,116 + Pf1N1B4_650 0.13 +2.5
744,117 - Pf1N1B4_650 0.13 +0.5
744,117 - Pf1N1B4_650 0.13 -0.0
744,117 - Pf1N1B4_650 0.13 +1.0
744,117 - +3.0
744,117 - Pf1N1B4_650 0.13 +3.3
744,117 - Pf1N1B4_650 0.13 +0.7
744,117 - Pf1N1B4_650 0.13 +0.7
744,117 - +1.0
744,289 + Pf1N1B4_650 0.48 +0.4
744,452 - Pf1N1B4_650 0.82 +0.3
744,541 + +0.2
744,541 + +1.7
744,541 + +1.2
744,541 + +2.0
744,541 + +0.4
744,541 + +0.4
744,542 - +1.5
744,542 - +1.0
744,542 - +1.2
744,542 - -0.4
744,542 - -0.0
744,542 - +1.0
744,542 - +1.5
744,554 - +0.2
744,554 - -0.8
744,598 - -1.6
744,612 + -0.1
744,612 + -0.6
744,612 + -0.6
744,613 - +0.6
744,613 - +1.0
744,613 - +1.1
744,613 - +1.0
744,613 - +1.7
744,613 - -0.4
744,613 - +3.3
744,613 - -0.0
744,613 - -0.0
744,615 - +1.0
744,615 - +1.1
744,615 - +0.5
744,615 - +1.0
744,696 + +1.2
744,696 + +1.1
744,697 - +0.7
744,697 - +1.7
744,697 - +0.4
744,697 - +0.6
744,697 - -1.0
744,697 - -0.9
744,697 - -1.6
744,791 + Pf1N1B4_651 0.12 +0.3
744,792 - Pf1N1B4_651 0.12 +0.1
744,792 - Pf1N1B4_651 0.12 +1.0
744,792 - +0.4
744,850 + Pf1N1B4_651 0.19 -0.0
744,850 + Pf1N1B4_651 0.19 +0.4
744,903 - Pf1N1B4_651 0.24 +0.6
744,903 - Pf1N1B4_651 0.24 +0.6
744,919 + Pf1N1B4_651 0.26 -0.2
744,919 + Pf1N1B4_651 0.26 +1.0
745,114 + Pf1N1B4_651 0.47 -0.2
745,115 - Pf1N1B4_651 0.47 +0.4
745,182 + Pf1N1B4_651 0.55 +1.6
745,438 + Pf1N1B4_651 0.83 -0.0
745,438 + Pf1N1B4_651 0.83 -0.1
745,438 + Pf1N1B4_651 0.83 -0.1
745,438 + Pf1N1B4_651 0.83 -0.3
745,439 - Pf1N1B4_651 0.83 +0.6
745,439 - Pf1N1B4_651 0.83 +0.7
745,477 + Pf1N1B4_651 0.87 -0.4
745,478 - Pf1N1B4_651 0.87 +0.4
745,478 - Pf1N1B4_651 0.87 +0.6
745,478 - Pf1N1B4_651 0.87 +0.5
745,478 - Pf1N1B4_651 0.87 +0.3
745,490 - Pf1N1B4_651 0.88 +0.6

Or see this region's nucleotide sequence