Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_644 and Pf1N1B4_645 are separated by 76 nucleotides Pf1N1B4_645 and Pf1N1B4_646 are separated by 3 nucleotides Pf1N1B4_646 and Pf1N1B4_647 are separated by 689 nucleotides Pf1N1B4_647 and Pf1N1B4_648 are separated by 6 nucleotides
Pf1N1B4_644 - Cold shock protein CspC, at 739,324 to 739,926
_644
Pf1N1B4_645 - Protein SlyX, at 740,003 to 740,209
_645
Pf1N1B4_646 - Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 740,213 to 740,638
_646
Pf1N1B4_647 - hypothetical protein, at 741,328 to 741,531
_647
Pf1N1B4_648 - Outer membrane porin, OprD family, at 741,538 to 742,866
_648
Position (kb) 740
741 Strain fitness (log2 ratio) -2
-1
0
1
2 at 739.223 kb on - strand at 739.347 kb on - strand at 739.409 kb on - strand, within Pf1N1B4_644 at 739.699 kb on + strand, within Pf1N1B4_644 at 739.700 kb on - strand, within Pf1N1B4_644 at 739.764 kb on + strand, within Pf1N1B4_644 at 739.765 kb on - strand, within Pf1N1B4_644 at 739.937 kb on - strand at 739.978 kb on - strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.390 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.628 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.806 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.100 kb on - strand at 741.111 kb on - strand at 741.194 kb on - strand at 741.194 kb on - strand at 741.194 kb on - strand at 741.196 kb on - strand at 741.223 kb on - strand at 741.232 kb on - strand at 741.367 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.421 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.445 kb on + strand, within Pf1N1B4_647 at 741.445 kb on + strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.486 kb on + strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.515 kb on - strand at 741.591 kb on + strand at 741.591 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 739,223 - -1.8 739,347 - +0.5 739,409 - Pf1N1B4_644 0.14 +0.4 739,699 + Pf1N1B4_644 0.62 +1.0 739,700 - Pf1N1B4_644 0.62 -1.0 739,764 + Pf1N1B4_644 0.73 +0.7 739,765 - Pf1N1B4_644 0.73 -1.8 739,937 - +0.2 739,978 - +1.0 740,007 + +0.0 740,007 + +0.5 740,007 + -0.6 740,007 + -0.2 740,008 - +2.1 740,008 - -0.2 740,008 - +0.7 740,008 - +0.4 740,293 - Pf1N1B4_646 0.19 +0.6 740,293 - Pf1N1B4_646 0.19 +1.4 740,293 - Pf1N1B4_646 0.19 +2.0 740,293 - Pf1N1B4_646 0.19 +0.4 740,293 - Pf1N1B4_646 0.19 -0.8 740,293 - Pf1N1B4_646 0.19 +1.3 740,390 - Pf1N1B4_646 0.42 +0.2 740,484 - Pf1N1B4_646 0.64 -1.4 740,484 - Pf1N1B4_646 0.64 +1.1 740,484 - Pf1N1B4_646 0.64 -0.2 740,493 - Pf1N1B4_646 0.66 +1.6 740,493 - Pf1N1B4_646 0.66 +1.3 740,493 - Pf1N1B4_646 0.66 -0.4 740,493 - Pf1N1B4_646 0.66 +1.5 740,493 - Pf1N1B4_646 0.66 +1.0 740,493 - Pf1N1B4_646 0.66 -0.9 740,493 - Pf1N1B4_646 0.66 +1.2 740,493 - Pf1N1B4_646 0.66 +0.3 740,493 - Pf1N1B4_646 0.66 +1.0 740,493 - Pf1N1B4_646 0.66 +0.8 740,493 - Pf1N1B4_646 0.66 +1.2 740,493 - Pf1N1B4_646 0.66 +1.4 740,493 - Pf1N1B4_646 0.66 +1.1 740,493 - Pf1N1B4_646 0.66 +1.2 740,493 - Pf1N1B4_646 0.66 +0.4 740,493 - Pf1N1B4_646 0.66 +1.3 740,502 - Pf1N1B4_646 0.68 +0.8 740,502 - Pf1N1B4_646 0.68 +0.7 740,502 - Pf1N1B4_646 0.68 -1.0 740,502 - Pf1N1B4_646 0.68 +1.7 740,502 - Pf1N1B4_646 0.68 +1.7 740,502 - Pf1N1B4_646 0.68 -1.2 740,502 - Pf1N1B4_646 0.68 -1.3 740,502 - Pf1N1B4_646 0.68 -2.4 740,502 - Pf1N1B4_646 0.68 +0.3 740,502 - Pf1N1B4_646 0.68 +2.4 740,502 - Pf1N1B4_646 0.68 +1.5 740,548 - Pf1N1B4_646 0.79 +0.4 740,548 - Pf1N1B4_646 0.79 +0.4 740,548 - Pf1N1B4_646 0.79 -0.1 740,548 - Pf1N1B4_646 0.79 +1.5 740,628 - +0.3 740,723 - -2.4 740,723 - +1.2 740,723 - -0.0 740,723 - +0.1 740,723 - -0.0 740,723 - -0.3 740,723 - +0.3 740,723 - +1.6 740,723 - +0.9 740,723 - +0.7 740,723 - +0.2 740,723 - +0.2 740,723 - +0.6 740,723 - -0.0 740,723 - +0.4 740,723 - +0.8 740,723 - -0.6 740,723 - -0.9 740,723 - +1.6 740,723 - +1.0 740,806 - +0.7 740,979 - -0.1 740,979 - -1.4 740,979 - +1.2 740,979 - +0.4 740,979 - +0.1 740,979 - +0.9 741,040 - -1.0 741,040 - -0.0 741,040 - +0.6 741,040 - +1.5 741,040 - -0.9 741,074 - +0.2 741,074 - +0.2 741,074 - +0.2 741,074 - -0.3 741,074 - +1.2 741,074 - -0.9 741,074 - +0.4 741,074 - -0.5 741,100 - -0.6 741,111 - -1.0 741,194 - -0.1 741,194 - -1.0 741,194 - -0.7 741,196 - -1.4 741,223 - -0.8 741,232 - -0.6 741,367 - Pf1N1B4_647 0.19 -1.0 741,409 - Pf1N1B4_647 0.40 +0.4 741,409 - Pf1N1B4_647 0.40 -0.5 741,409 - Pf1N1B4_647 0.40 +0.7 741,409 - Pf1N1B4_647 0.40 -0.6 741,421 - Pf1N1B4_647 0.46 -2.6 741,444 - Pf1N1B4_647 0.57 -1.0 741,444 - Pf1N1B4_647 0.57 +1.2 741,444 - Pf1N1B4_647 0.57 -0.2 741,444 - Pf1N1B4_647 0.57 -0.2 741,444 - Pf1N1B4_647 0.57 +0.7 741,445 + Pf1N1B4_647 0.57 -1.6 741,445 + Pf1N1B4_647 0.57 +0.4 741,446 - Pf1N1B4_647 0.58 +0.3 741,446 - Pf1N1B4_647 0.58 -1.1 741,446 - Pf1N1B4_647 0.58 +2.0 741,446 - Pf1N1B4_647 0.58 -0.6 741,446 - Pf1N1B4_647 0.58 -0.7 741,446 - Pf1N1B4_647 0.58 -1.4 741,446 - Pf1N1B4_647 0.58 -0.6 741,446 - Pf1N1B4_647 0.58 +1.2 741,446 - -0.0 741,446 - Pf1N1B4_647 0.58 +0.2 741,446 - Pf1N1B4_647 0.58 +1.2 741,446 - Pf1N1B4_647 0.58 -0.0 741,446 - Pf1N1B4_647 0.58 -0.3 741,446 - Pf1N1B4_647 0.58 +0.2 741,446 - Pf1N1B4_647 0.58 +1.0 741,446 - Pf1N1B4_647 0.58 -1.0 741,486 + Pf1N1B4_647 0.77 +1.2 741,487 - Pf1N1B4_647 0.78 +0.2 741,487 - Pf1N1B4_647 0.78 +1.0 741,487 - Pf1N1B4_647 0.78 +1.0 741,487 - Pf1N1B4_647 0.78 -0.4 741,487 - Pf1N1B4_647 0.78 -0.1 741,515 - +1.7 741,591 + -0.8 741,591 + -1.5 741,620 + +0.6 741,620 + -0.7 741,620 + -1.9 741,620 + -1.0 741,620 + +0.1 741,620 + -0.8 741,621 - -0.9 741,621 - +0.6 741,621 - -0.4 741,621 - -0.6 741,621 - +0.7 741,621 - -1.0 741,621 - -0.6 741,621 - -0.2
Or see this region's nucleotide sequence