Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_628 and Pf1N1B4_629 are separated by 13 nucleotides Pf1N1B4_629 and Pf1N1B4_630 are separated by 166 nucleotides Pf1N1B4_630 and Pf1N1B4_631 are separated by 81 nucleotides Pf1N1B4_631 and Pf1N1B4_632 are separated by 52 nucleotides
Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560
_628
Pf1N1B4_629 - Queuosine Biosynthesis QueE Radical SAM, at 726,574 to 727,221
_629
Pf1N1B4_630 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 727,388 to 728,152
_630
Pf1N1B4_631 - 18K peptidoglycan-associated outer membrane lipoprotein; Peptidoglycan-associated lipoprotein precursor; Outer membrane protein P6; OmpA/MotB precursor, at 728,234 to 728,731
_631
Pf1N1B4_632 - tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins, at 728,784 to 730,052
_632
Position (kb) 727
728
729 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 726.446 kb on - strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.602 kb on + strand at 726.602 kb on + strand at 726.602 kb on + strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.761 kb on + strand, within Pf1N1B4_629 at 726.791 kb on - strand, within Pf1N1B4_629 at 727.019 kb on + strand, within Pf1N1B4_629 at 727.020 kb on - strand, within Pf1N1B4_629 at 727.221 kb on + strand at 727.221 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.244 kb on - strand at 727.244 kb on - strand at 727.318 kb on - strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.519 kb on - strand, within Pf1N1B4_630 at 727.740 kb on + strand at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.867 kb on + strand, within Pf1N1B4_630 at 727.901 kb on + strand, within Pf1N1B4_630 at 727.902 kb on - strand, within Pf1N1B4_630 at 728.077 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.129 kb on - strand at 728.129 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.235 kb on + strand at 728.236 kb on - strand at 728.236 kb on - strand at 728.236 kb on - strand at 728.236 kb on - strand at 728.236 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.240 kb on - strand at 728.762 kb on - strand at 728.766 kb on - strand at 728.766 kb on - strand at 728.766 kb on - strand at 728.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 726,446 - Pf1N1B4_628 0.83 +2.3 726,463 + Pf1N1B4_628 0.86 +1.0 726,463 + Pf1N1B4_628 0.86 -0.8 726,463 + Pf1N1B4_628 0.86 +0.4 726,464 - Pf1N1B4_628 0.86 -0.2 726,464 - Pf1N1B4_628 0.86 -0.2 726,602 + +0.6 726,602 + -0.6 726,602 + +1.4 726,603 - -0.6 726,603 - +0.2 726,603 - -0.0 726,603 - -0.5 726,606 + -1.6 726,606 + -0.3 726,606 + +0.5 726,606 + -0.6 726,606 + -0.9 726,606 + -0.4 726,607 - +0.8 726,607 - +1.5 726,607 - -0.0 726,607 - -0.8 726,607 - -1.4 726,607 - -1.4 726,607 - -1.2 726,607 - -1.2 726,607 - -1.4 726,607 - -1.0 726,607 - +1.4 726,761 + Pf1N1B4_629 0.29 -0.4 726,791 - Pf1N1B4_629 0.33 -2.4 727,019 + Pf1N1B4_629 0.69 +0.8 727,020 - Pf1N1B4_629 0.69 -0.6 727,221 + +0.8 727,221 + +1.6 727,243 + -2.4 727,243 + -0.0 727,243 + -0.3 727,244 - +0.2 727,244 - -2.0 727,318 - +2.5 727,389 + -1.0 727,389 + -0.8 727,389 + -1.0 727,390 - -0.8 727,390 - -0.4 727,390 - +0.5 727,390 - -0.0 727,390 - -0.9 727,390 - +0.4 727,390 - -0.0 727,390 - +2.0 727,519 - Pf1N1B4_630 0.17 -1.0 727,740 + +1.0 727,740 + Pf1N1B4_630 0.46 -1.8 727,740 + Pf1N1B4_630 0.46 -1.4 727,740 + Pf1N1B4_630 0.46 -0.8 727,741 - Pf1N1B4_630 0.46 -0.6 727,741 - Pf1N1B4_630 0.46 -2.6 727,741 - Pf1N1B4_630 0.46 +0.7 727,741 - Pf1N1B4_630 0.46 -2.0 727,741 - Pf1N1B4_630 0.46 -0.9 727,741 - Pf1N1B4_630 0.46 +0.4 727,744 - Pf1N1B4_630 0.47 +0.2 727,744 - Pf1N1B4_630 0.47 -0.6 727,744 - Pf1N1B4_630 0.47 -0.1 727,744 - Pf1N1B4_630 0.47 +1.1 727,867 + Pf1N1B4_630 0.63 -0.6 727,901 + Pf1N1B4_630 0.67 -2.1 727,902 - Pf1N1B4_630 0.67 -0.0 728,077 - -2.0 728,114 - -1.8 728,114 - -1.8 728,114 - -2.2 728,114 - -1.8 728,114 - -1.0 728,114 - -1.6 728,114 - -1.6 728,129 - -1.8 728,129 - -1.6 728,205 - -1.0 728,205 - -3.2 728,205 - -1.8 728,205 - -3.1 728,205 - +1.0 728,205 - +1.5 728,235 + -1.8 728,236 - -1.6 728,236 - -0.8 728,236 - -2.7 728,236 - -0.2 728,236 - -1.2 728,240 - -0.3 728,240 - +1.5 728,240 - -1.0 728,240 - -0.0 728,240 - +0.2 728,240 - -1.1 728,240 - -0.6 728,240 - -0.0 728,240 - +0.2 728,240 - +0.4 728,240 - -0.6 728,240 - -0.5 728,762 - +0.1 728,766 - +1.4 728,766 - +1.0 728,766 - +0.3 728,795 - +0.6
Or see this region's nucleotide sequence