Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_612

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_611 and Pf1N1B4_612 are separated by 133 nucleotidesPf1N1B4_612 and Pf1N1B4_613 overlap by 4 nucleotides Pf1N1B4_611 - CoA transferase, CAIB/BAIF family, at 705,554 to 706,885 _611 Pf1N1B4_612 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 707,019 to 707,882 _612 Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306 _613 Position (kb) 707 708Strain fitness (log2 ratio) -2 -1 0 1 2 3at 706.516 kb on - strand, within Pf1N1B4_611at 706.613 kb on + strand, within Pf1N1B4_611at 706.997 kb on - strandat 707.063 kb on - strandat 707.209 kb on + strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.397 kb on + strand, within Pf1N1B4_612at 707.397 kb on + strand, within Pf1N1B4_612at 707.398 kb on - strandat 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.777 kb on - strand, within Pf1N1B4_612at 707.851 kb on + strandat 707.852 kb on - strandat 707.901 kb on + strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.969 kb on - strandat 708.027 kb on + strand, within Pf1N1B4_613at 708.028 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.181 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.228 kb on + strand, within Pf1N1B4_613at 708.228 kb on + strand, within Pf1N1B4_613at 708.229 kb on - strand, within Pf1N1B4_613at 708.229 kb on - strandat 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.437 kb on - strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.482 kb on - strand, within Pf1N1B4_613at 708.592 kb on + strand, within Pf1N1B4_613at 708.649 kb on - strand, within Pf1N1B4_613

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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706,516 - Pf1N1B4_611 0.72 +0.8
706,613 + Pf1N1B4_611 0.80 -1.6
706,997 - -2.2
707,063 - +1.0
707,209 + Pf1N1B4_612 0.22 +1.3
707,210 - Pf1N1B4_612 0.22 +0.6
707,210 - Pf1N1B4_612 0.22 +0.6
707,210 - Pf1N1B4_612 0.22 -1.0
707,210 - Pf1N1B4_612 0.22 +1.0
707,210 - Pf1N1B4_612 0.22 -1.0
707,397 + Pf1N1B4_612 0.44 +0.4
707,397 + Pf1N1B4_612 0.44 +2.1
707,398 - +2.0
707,398 - Pf1N1B4_612 0.44 -2.0
707,398 - Pf1N1B4_612 0.44 -1.4
707,398 - Pf1N1B4_612 0.44 +0.4
707,398 - Pf1N1B4_612 0.44 +1.2
707,398 - Pf1N1B4_612 0.44 -0.2
707,777 - Pf1N1B4_612 0.88 +0.4
707,851 + -0.6
707,852 - -0.3
707,901 + -0.0
707,902 - +0.6
707,902 - +0.4
707,902 - -1.8
707,902 - -0.4
707,902 - -0.2
707,902 - +0.6
707,969 - +0.1
708,027 + Pf1N1B4_613 0.10 +2.4
708,028 - Pf1N1B4_613 0.10 +1.7
708,070 - Pf1N1B4_613 0.13 -1.6
708,070 - Pf1N1B4_613 0.13 -0.6
708,070 - Pf1N1B4_613 0.13 -0.6
708,070 - Pf1N1B4_613 0.13 -1.0
708,148 - Pf1N1B4_613 0.19 -1.0
708,148 - Pf1N1B4_613 0.19 -0.3
708,148 - Pf1N1B4_613 0.19 -2.4
708,148 - Pf1N1B4_613 0.19 +0.6
708,148 - Pf1N1B4_613 0.19 +0.6
708,181 - Pf1N1B4_613 0.21 +3.0
708,199 - Pf1N1B4_613 0.22 -1.6
708,199 - Pf1N1B4_613 0.22 +0.4
708,199 - Pf1N1B4_613 0.22 +0.5
708,199 - Pf1N1B4_613 0.22 -0.6
708,228 + Pf1N1B4_613 0.24 -1.4
708,228 + Pf1N1B4_613 0.24 +0.3
708,229 - Pf1N1B4_613 0.25 +1.5
708,229 - +0.6
708,243 + Pf1N1B4_613 0.25 -0.5
708,243 + Pf1N1B4_613 0.25 -0.0
708,243 + Pf1N1B4_613 0.25 +0.2
708,243 + Pf1N1B4_613 0.25 -0.7
708,243 + Pf1N1B4_613 0.25 -1.0
708,243 + Pf1N1B4_613 0.25 -2.2
708,243 + Pf1N1B4_613 0.25 +0.8
708,244 - Pf1N1B4_613 0.26 -0.6
708,244 - Pf1N1B4_613 0.26 +0.3
708,244 - Pf1N1B4_613 0.26 +0.1
708,244 - Pf1N1B4_613 0.26 +2.8
708,437 - Pf1N1B4_613 0.39 +0.6
708,481 + Pf1N1B4_613 0.42 +0.6
708,481 + Pf1N1B4_613 0.42 -0.4
708,481 + Pf1N1B4_613 0.42 +1.5
708,482 - Pf1N1B4_613 0.42 -1.0
708,592 + Pf1N1B4_613 0.50 -1.6
708,649 - Pf1N1B4_613 0.54 -1.6

Or see this region's nucleotide sequence