Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5984 and Pf1N1B4_5985 are separated by 15 nucleotides Pf1N1B4_5985 and Pf1N1B4_5986 are separated by 150 nucleotides
Pf1N1B4_5984 - FIG005548: transport protein, at 434,127 to 436,643
_5984
Pf1N1B4_5985 - FIG002465: BNR repeat protein, at 436,659 to 437,747
_5985
Pf1N1B4_5986 - FIG002188: hypothetical protein, at 437,898 to 439,256
_5986
Position (kb) 436
437
438 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 435.815 kb on - strand, within Pf1N1B4_5984 at 436.108 kb on - strand, within Pf1N1B4_5984 at 436.155 kb on + strand, within Pf1N1B4_5984 at 436.177 kb on + strand, within Pf1N1B4_5984 at 436.177 kb on + strand, within Pf1N1B4_5984 at 436.429 kb on + strand at 436.495 kb on + strand at 436.495 kb on + strand at 436.525 kb on - strand at 436.525 kb on - strand at 436.546 kb on + strand at 436.676 kb on + strand at 436.836 kb on + strand, within Pf1N1B4_5985 at 437.008 kb on + strand, within Pf1N1B4_5985 at 437.009 kb on - strand, within Pf1N1B4_5985 at 437.084 kb on - strand, within Pf1N1B4_5985 at 437.084 kb on - strand, within Pf1N1B4_5985 at 437.215 kb on + strand, within Pf1N1B4_5985 at 437.666 kb on - strand at 437.697 kb on + strand at 437.697 kb on + strand at 437.697 kb on + strand at 437.728 kb on + strand at 437.729 kb on - strand at 437.729 kb on - strand at 437.738 kb on - strand at 437.747 kb on + strand at 437.963 kb on - strand at 437.963 kb on - strand at 437.963 kb on - strand at 437.963 kb on - strand at 438.005 kb on - strand at 438.005 kb on - strand at 438.005 kb on - strand at 438.025 kb on + strand at 438.026 kb on - strand at 438.026 kb on - strand at 438.026 kb on - strand at 438.026 kb on - strand at 438.065 kb on - strand, within Pf1N1B4_5986 at 438.065 kb on - strand, within Pf1N1B4_5986 at 438.097 kb on + strand, within Pf1N1B4_5986 at 438.118 kb on + strand, within Pf1N1B4_5986 at 438.119 kb on - strand, within Pf1N1B4_5986 at 438.160 kb on + strand, within Pf1N1B4_5986 at 438.160 kb on + strand, within Pf1N1B4_5986 at 438.161 kb on - strand, within Pf1N1B4_5986 at 438.178 kb on + strand, within Pf1N1B4_5986 at 438.178 kb on + strand, within Pf1N1B4_5986 at 438.178 kb on + strand, within Pf1N1B4_5986 at 438.179 kb on - strand, within Pf1N1B4_5986 at 438.179 kb on - strand, within Pf1N1B4_5986 at 438.299 kb on - strand, within Pf1N1B4_5986 at 438.310 kb on + strand, within Pf1N1B4_5986 at 438.310 kb on + strand, within Pf1N1B4_5986 at 438.311 kb on - strand, within Pf1N1B4_5986 at 438.311 kb on - strand, within Pf1N1B4_5986 at 438.311 kb on - strand, within Pf1N1B4_5986 at 438.320 kb on - strand, within Pf1N1B4_5986 at 438.400 kb on + strand, within Pf1N1B4_5986 at 438.484 kb on + strand, within Pf1N1B4_5986 at 438.571 kb on + strand at 438.592 kb on + strand, within Pf1N1B4_5986 at 438.592 kb on + strand, within Pf1N1B4_5986 at 438.593 kb on - strand, within Pf1N1B4_5986 at 438.593 kb on - strand, within Pf1N1B4_5986 at 438.593 kb on - strand, within Pf1N1B4_5986 at 438.710 kb on - strand, within Pf1N1B4_5986 at 438.710 kb on - strand, within Pf1N1B4_5986 at 438.710 kb on - strand, within Pf1N1B4_5986 at 438.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 435,815 - Pf1N1B4_5984 0.67 -0.8 436,108 - Pf1N1B4_5984 0.79 +0.7 436,155 + Pf1N1B4_5984 0.81 -0.8 436,177 + Pf1N1B4_5984 0.81 -0.5 436,177 + Pf1N1B4_5984 0.81 +0.8 436,429 + -0.2 436,495 + -0.5 436,495 + +2.4 436,525 - -0.2 436,525 - +0.0 436,546 + +0.8 436,676 + +2.3 436,836 + Pf1N1B4_5985 0.16 +0.5 437,008 + Pf1N1B4_5985 0.32 +2.2 437,009 - Pf1N1B4_5985 0.32 +0.8 437,084 - Pf1N1B4_5985 0.39 +0.1 437,084 - Pf1N1B4_5985 0.39 +1.2 437,215 + Pf1N1B4_5985 0.51 +1.4 437,666 - -0.3 437,697 + -0.8 437,697 + -0.5 437,697 + -0.7 437,728 + -2.8 437,729 - -2.2 437,729 - +0.2 437,738 - +0.2 437,747 + -0.3 437,963 - -0.5 437,963 - -0.5 437,963 - -1.0 437,963 - +0.1 438,005 - -0.5 438,005 - +0.1 438,005 - +1.0 438,025 + +2.6 438,026 - +1.6 438,026 - +1.3 438,026 - +2.2 438,026 - +0.4 438,065 - Pf1N1B4_5986 0.12 +1.3 438,065 - Pf1N1B4_5986 0.12 +0.2 438,097 + Pf1N1B4_5986 0.15 +0.4 438,118 + Pf1N1B4_5986 0.16 -0.3 438,119 - Pf1N1B4_5986 0.16 +1.2 438,160 + Pf1N1B4_5986 0.19 +1.1 438,160 + Pf1N1B4_5986 0.19 +0.2 438,161 - Pf1N1B4_5986 0.19 +0.2 438,178 + Pf1N1B4_5986 0.21 +1.8 438,178 + Pf1N1B4_5986 0.21 -0.6 438,178 + Pf1N1B4_5986 0.21 -0.5 438,179 - Pf1N1B4_5986 0.21 -0.1 438,179 - Pf1N1B4_5986 0.21 +1.1 438,299 - Pf1N1B4_5986 0.30 +1.5 438,310 + Pf1N1B4_5986 0.30 +1.2 438,310 + Pf1N1B4_5986 0.30 -0.6 438,311 - Pf1N1B4_5986 0.30 +1.1 438,311 - Pf1N1B4_5986 0.30 +1.4 438,311 - Pf1N1B4_5986 0.30 +1.6 438,320 - Pf1N1B4_5986 0.31 +0.8 438,400 + Pf1N1B4_5986 0.37 -0.8 438,484 + Pf1N1B4_5986 0.43 -1.6 438,571 + +0.8 438,592 + Pf1N1B4_5986 0.51 +0.7 438,592 + Pf1N1B4_5986 0.51 -0.2 438,593 - Pf1N1B4_5986 0.51 -0.5 438,593 - Pf1N1B4_5986 0.51 -0.2 438,593 - Pf1N1B4_5986 0.51 +0.4 438,710 - Pf1N1B4_5986 0.60 +1.2 438,710 - Pf1N1B4_5986 0.60 +1.6 438,710 - Pf1N1B4_5986 0.60 -1.5 438,710 - +1.2
Or see this region's nucleotide sequence