Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5862 and Pf1N1B4_5863 overlap by 1 nucleotides Pf1N1B4_5863 and Pf1N1B4_5864 are separated by 1 nucleotides
Pf1N1B4_5862 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 296,341 to 297,840
_5862
Pf1N1B4_5863 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 297,840 to 298,889
_5863
Pf1N1B4_5864 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 298,891 to 300,108
_5864
Position (kb) 297
298
299 Strain fitness (log2 ratio) -1
0
1
2
3 at 296.854 kb on + strand, within Pf1N1B4_5862 at 296.867 kb on + strand, within Pf1N1B4_5862 at 296.868 kb on - strand, within Pf1N1B4_5862 at 297.395 kb on - strand, within Pf1N1B4_5862 at 297.513 kb on + strand, within Pf1N1B4_5862 at 297.514 kb on - strand, within Pf1N1B4_5862 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.306 kb on - strand, within Pf1N1B4_5863 at 298.554 kb on + strand at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.887 kb on + strand at 298.887 kb on + strand at 298.888 kb on - strand at 298.890 kb on + strand at 298.891 kb on - strand at 299.002 kb on + strand at 299.003 kb on - strand at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.048 kb on - strand, within Pf1N1B4_5864 at 299.531 kb on - strand, within Pf1N1B4_5864 at 299.692 kb on + strand, within Pf1N1B4_5864 at 299.693 kb on - strand, within Pf1N1B4_5864 at 299.814 kb on + strand at 299.849 kb on + strand, within Pf1N1B4_5864
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 296,854 + Pf1N1B4_5862 0.34 +0.1 296,867 + Pf1N1B4_5862 0.35 +3.2 296,868 - Pf1N1B4_5862 0.35 +1.8 297,395 - Pf1N1B4_5862 0.70 +0.9 297,513 + Pf1N1B4_5862 0.78 -1.3 297,514 - Pf1N1B4_5862 0.78 -1.5 298,003 - Pf1N1B4_5863 0.16 +0.8 298,003 - Pf1N1B4_5863 0.16 +0.4 298,306 - Pf1N1B4_5863 0.44 +1.1 298,554 + -0.8 298,583 - Pf1N1B4_5863 0.71 +1.1 298,583 - Pf1N1B4_5863 0.71 +1.0 298,887 + +0.5 298,887 + -0.2 298,888 - +0.7 298,890 + +0.8 298,891 - -0.2 299,002 + -1.0 299,003 - +2.1 299,047 + Pf1N1B4_5864 0.13 -0.2 299,047 + Pf1N1B4_5864 0.13 +0.8 299,048 - Pf1N1B4_5864 0.13 +2.8 299,531 - Pf1N1B4_5864 0.53 -0.2 299,692 + Pf1N1B4_5864 0.66 -1.2 299,693 - Pf1N1B4_5864 0.66 -1.0 299,814 + -0.2 299,849 + Pf1N1B4_5864 0.79 -1.2
Or see this region's nucleotide sequence