Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5784 and Pf1N1B4_5785 overlap by 28 nucleotides Pf1N1B4_5785 and Pf1N1B4_5786 are separated by 579 nucleotides Pf1N1B4_5786 and Pf1N1B4_5787 are separated by 219 nucleotides
Pf1N1B4_5784 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 223,540 to 224,085
_5784
Pf1N1B4_5785 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 224,058 to 224,423
_5785
Pf1N1B4_5786 - putative membrane protein, at 225,003 to 225,446
_5786
Pf1N1B4_5787 - Gifsy-2 prophage protein, at 225,666 to 226,358
_5787
Position (kb) 225
226 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.040 kb on - strand at 224.165 kb on - strand, within Pf1N1B4_5785 at 224.165 kb on - strand at 224.335 kb on + strand, within Pf1N1B4_5785 at 224.464 kb on + strand at 224.464 kb on + strand at 224.465 kb on - strand at 224.781 kb on + strand at 224.781 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.954 kb on - strand at 225.016 kb on + strand at 225.016 kb on + strand at 225.017 kb on - strand at 225.226 kb on + strand at 225.330 kb on + strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.628 kb on + strand at 225.636 kb on - strand at 225.636 kb on - strand at 225.641 kb on + strand at 225.641 kb on + strand at 225.743 kb on + strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.937 kb on + strand, within Pf1N1B4_5787 at 225.938 kb on - strand, within Pf1N1B4_5787 at 225.956 kb on + strand, within Pf1N1B4_5787 at 225.957 kb on - strand at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.988 kb on - strand, within Pf1N1B4_5787 at 226.015 kb on - strand at 226.078 kb on - strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.238 kb on - strand, within Pf1N1B4_5787
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 224,039 + +0.2 224,039 + -1.2 224,039 + +0.8 224,039 + +0.8 224,040 - -1.2 224,165 - Pf1N1B4_5785 0.29 -1.2 224,165 - -0.2 224,335 + Pf1N1B4_5785 0.76 +0.4 224,464 + -1.4 224,464 + -1.5 224,465 - +0.8 224,781 + -0.2 224,781 + +2.2 224,953 + +0.5 224,953 + +0.8 224,953 + -2.0 224,954 - +1.6 225,016 + +0.6 225,016 + -0.2 225,017 - +0.8 225,226 + -0.2 225,330 + Pf1N1B4_5786 0.74 -1.7 225,331 - Pf1N1B4_5786 0.74 -0.2 225,331 - +0.8 225,331 - Pf1N1B4_5786 0.74 -0.8 225,331 - Pf1N1B4_5786 0.74 -1.0 225,628 + +0.1 225,636 - +1.1 225,636 - +1.4 225,641 + +1.5 225,641 + +0.1 225,743 + Pf1N1B4_5787 0.11 +0.2 225,772 - Pf1N1B4_5787 0.15 -1.0 225,772 - Pf1N1B4_5787 0.15 +0.8 225,937 + Pf1N1B4_5787 0.39 +0.6 225,938 - Pf1N1B4_5787 0.39 -0.8 225,956 + Pf1N1B4_5787 0.42 -1.2 225,957 - -1.2 225,957 - Pf1N1B4_5787 0.42 -1.2 225,957 - Pf1N1B4_5787 0.42 -2.0 225,957 - Pf1N1B4_5787 0.42 -1.2 225,988 - Pf1N1B4_5787 0.46 +0.8 226,015 - +0.8 226,078 - Pf1N1B4_5787 0.59 +1.8 226,237 + Pf1N1B4_5787 0.82 +0.5 226,237 + Pf1N1B4_5787 0.82 +0.8 226,238 - Pf1N1B4_5787 0.83 +1.4
Or see this region's nucleotide sequence