Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5665 and Pf1N1B4_5666 overlap by 17 nucleotides Pf1N1B4_5666 and Pf1N1B4_5667 are separated by 44 nucleotides
Pf1N1B4_5665 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 98,746 to 100,404
_5665
Pf1N1B4_5666 - Oxidoreductase probably involved in sulfite reduction, at 100,388 to 100,882
_5666
Pf1N1B4_5667 - hypothetical protein, at 100,927 to 104,862
_5667
Position (kb) 100
101 Strain fitness (log2 ratio) -2
-1
0
1
2 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.431 kb on - strand, within Pf1N1B4_5665 at 99.455 kb on - strand, within Pf1N1B4_5665 at 99.484 kb on + strand, within Pf1N1B4_5665 at 99.484 kb on + strand at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.745 kb on + strand, within Pf1N1B4_5665 at 100.075 kb on + strand, within Pf1N1B4_5665 at 100.076 kb on - strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.349 kb on - strand at 100.388 kb on - strand at 100.399 kb on - strand at 100.399 kb on - strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.403 kb on - strand at 100.522 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.796 kb on + strand, within Pf1N1B4_5666 at 100.835 kb on + strand at 100.835 kb on + strand at 100.928 kb on + strand at 100.929 kb on - strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.137 kb on - strand at 101.137 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.270 kb on - strand at 101.514 kb on + strand, within Pf1N1B4_5667 at 101.515 kb on - strand, within Pf1N1B4_5667 at 101.515 kb on - strand, within Pf1N1B4_5667 at 101.620 kb on - strand, within Pf1N1B4_5667 at 101.751 kb on + strand, within Pf1N1B4_5667
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 99,430 + Pf1N1B4_5665 0.41 +1.4 99,430 + Pf1N1B4_5665 0.41 -0.2 99,430 + Pf1N1B4_5665 0.41 -1.8 99,431 - Pf1N1B4_5665 0.41 +0.3 99,455 - Pf1N1B4_5665 0.43 -1.0 99,484 + Pf1N1B4_5665 0.44 -0.8 99,484 + +2.0 99,485 - Pf1N1B4_5665 0.45 -0.4 99,485 - Pf1N1B4_5665 0.45 -1.2 99,745 + Pf1N1B4_5665 0.60 -0.9 100,075 + Pf1N1B4_5665 0.80 -0.2 100,076 - Pf1N1B4_5665 0.80 -0.8 100,192 + Pf1N1B4_5665 0.87 +0.6 100,192 + Pf1N1B4_5665 0.87 -0.8 100,192 + Pf1N1B4_5665 0.87 -1.5 100,306 + -2.0 100,306 + -0.2 100,306 + -1.2 100,306 + -0.5 100,306 + +2.2 100,306 + -0.4 100,348 + -0.8 100,348 + +0.8 100,348 + -1.5 100,348 + +1.8 100,348 + +0.0 100,349 - -2.4 100,388 - +0.8 100,399 - -1.2 100,399 - -1.2 100,402 + -0.9 100,402 + -0.9 100,402 + +0.1 100,403 - -0.2 100,522 + Pf1N1B4_5666 0.27 -1.2 100,655 + Pf1N1B4_5666 0.54 -0.5 100,655 + Pf1N1B4_5666 0.54 -0.1 100,655 + Pf1N1B4_5666 0.54 -1.5 100,742 + Pf1N1B4_5666 0.72 -0.2 100,742 + Pf1N1B4_5666 0.72 -2.0 100,742 + Pf1N1B4_5666 0.72 -1.2 100,796 + Pf1N1B4_5666 0.82 -0.5 100,835 + -1.0 100,835 + +0.2 100,928 + +0.8 100,929 - -0.5 101,136 + +0.8 101,136 + -0.8 101,136 + -0.2 101,137 - -0.2 101,137 - -0.2 101,139 - -0.2 101,139 - -0.8 101,139 - -1.4 101,270 - -0.2 101,514 + Pf1N1B4_5667 0.15 +1.8 101,515 - Pf1N1B4_5667 0.15 -1.4 101,515 - Pf1N1B4_5667 0.15 +0.1 101,620 - Pf1N1B4_5667 0.18 +2.4 101,751 + Pf1N1B4_5667 0.21 -2.5
Or see this region's nucleotide sequence