Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5660 and Pf1N1B4_5661 are separated by 155 nucleotides Pf1N1B4_5661 and Pf1N1B4_5662 are separated by 156 nucleotides Pf1N1B4_5662 and Pf1N1B4_5663 are separated by 6 nucleotides
Pf1N1B4_5660 - cis/trans isomerase, at 90,603 to 92,888
_5660
Pf1N1B4_5661 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 93,044 to 96,754
_5661
Pf1N1B4_5662 - FIG00953703: hypothetical protein, at 96,911 to 97,132
_5662
Pf1N1B4_5663 - Periplasmic sugar-binding domain protein, at 97,139 to 98,149
_5663
Position (kb)
93
94
95
96
97 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 92.068 kb on + strand, within Pf1N1B4_5660 at 92.071 kb on + strand, within Pf1N1B4_5660 at 92.071 kb on + strand, within Pf1N1B4_5660 at 92.071 kb on + strand at 92.071 kb on + strand, within Pf1N1B4_5660 at 92.071 kb on + strand, within Pf1N1B4_5660 at 92.072 kb on - strand, within Pf1N1B4_5660 at 92.072 kb on - strand, within Pf1N1B4_5660 at 92.072 kb on - strand, within Pf1N1B4_5660 at 92.072 kb on - strand, within Pf1N1B4_5660 at 92.191 kb on + strand, within Pf1N1B4_5660 at 92.191 kb on + strand, within Pf1N1B4_5660 at 92.191 kb on + strand, within Pf1N1B4_5660 at 92.192 kb on - strand, within Pf1N1B4_5660 at 92.368 kb on + strand, within Pf1N1B4_5660 at 92.414 kb on + strand, within Pf1N1B4_5660 at 92.594 kb on - strand at 92.635 kb on + strand at 92.680 kb on + strand at 92.753 kb on - strand at 92.848 kb on - strand at 92.888 kb on + strand at 92.889 kb on - strand at 93.124 kb on + strand at 93.140 kb on + strand at 93.141 kb on - strand at 93.141 kb on - strand at 93.141 kb on - strand at 93.141 kb on - strand at 93.161 kb on + strand at 93.366 kb on + strand at 93.367 kb on - strand at 93.539 kb on + strand, within Pf1N1B4_5661 at 93.902 kb on + strand, within Pf1N1B4_5661 at 94.190 kb on + strand, within Pf1N1B4_5661 at 94.190 kb on + strand, within Pf1N1B4_5661 at 94.190 kb on + strand, within Pf1N1B4_5661 at 94.190 kb on + strand, within Pf1N1B4_5661 at 94.191 kb on - strand, within Pf1N1B4_5661 at 94.191 kb on - strand, within Pf1N1B4_5661 at 94.575 kb on + strand, within Pf1N1B4_5661 at 94.576 kb on - strand, within Pf1N1B4_5661 at 94.664 kb on + strand, within Pf1N1B4_5661 at 94.664 kb on + strand, within Pf1N1B4_5661 at 94.664 kb on + strand at 94.665 kb on - strand at 94.665 kb on - strand at 94.808 kb on + strand, within Pf1N1B4_5661 at 94.808 kb on + strand, within Pf1N1B4_5661 at 94.808 kb on + strand, within Pf1N1B4_5661 at 94.809 kb on - strand, within Pf1N1B4_5661 at 94.809 kb on - strand, within Pf1N1B4_5661 at 94.865 kb on + strand, within Pf1N1B4_5661 at 94.865 kb on + strand at 94.865 kb on + strand, within Pf1N1B4_5661 at 94.865 kb on + strand, within Pf1N1B4_5661 at 94.865 kb on + strand, within Pf1N1B4_5661 at 94.866 kb on - strand, within Pf1N1B4_5661 at 94.866 kb on - strand, within Pf1N1B4_5661 at 94.866 kb on - strand, within Pf1N1B4_5661 at 94.866 kb on - strand, within Pf1N1B4_5661 at 94.866 kb on - strand, within Pf1N1B4_5661 at 94.964 kb on + strand, within Pf1N1B4_5661 at 94.964 kb on + strand, within Pf1N1B4_5661 at 94.964 kb on + strand, within Pf1N1B4_5661 at 94.964 kb on + strand, within Pf1N1B4_5661 at 94.965 kb on - strand, within Pf1N1B4_5661 at 94.965 kb on - strand, within Pf1N1B4_5661 at 94.965 kb on - strand, within Pf1N1B4_5661 at 94.965 kb on - strand, within Pf1N1B4_5661 at 95.322 kb on + strand, within Pf1N1B4_5661 at 95.322 kb on + strand, within Pf1N1B4_5661 at 95.323 kb on - strand, within Pf1N1B4_5661 at 95.323 kb on - strand at 95.618 kb on + strand, within Pf1N1B4_5661 at 95.619 kb on - strand, within Pf1N1B4_5661 at 95.619 kb on - strand at 95.711 kb on + strand, within Pf1N1B4_5661 at 95.711 kb on + strand, within Pf1N1B4_5661 at 95.711 kb on + strand, within Pf1N1B4_5661 at 95.712 kb on - strand at 95.885 kb on + strand, within Pf1N1B4_5661 at 95.885 kb on + strand, within Pf1N1B4_5661 at 96.224 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.379 kb on + strand, within Pf1N1B4_5661 at 96.386 kb on + strand at 96.386 kb on + strand at 96.386 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.578 kb on + strand at 96.614 kb on + strand at 96.615 kb on - strand at 96.615 kb on - strand at 96.639 kb on - strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.753 kb on - strand at 96.868 kb on + strand at 96.869 kb on - strand at 96.869 kb on - strand at 97.075 kb on - strand, within Pf1N1B4_5662 at 97.444 kb on + strand, within Pf1N1B4_5663 at 97.498 kb on - strand, within Pf1N1B4_5663
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 92,068 + Pf1N1B4_5660 0.64 +1.3 92,071 + Pf1N1B4_5660 0.64 -1.8 92,071 + Pf1N1B4_5660 0.64 -0.5 92,071 + -0.2 92,071 + Pf1N1B4_5660 0.64 +0.8 92,071 + Pf1N1B4_5660 0.64 -1.7 92,072 - Pf1N1B4_5660 0.64 +0.4 92,072 - Pf1N1B4_5660 0.64 +1.8 92,072 - Pf1N1B4_5660 0.64 +0.6 92,072 - Pf1N1B4_5660 0.64 -0.1 92,191 + Pf1N1B4_5660 0.69 -0.0 92,191 + Pf1N1B4_5660 0.69 +0.1 92,191 + Pf1N1B4_5660 0.69 +0.8 92,192 - Pf1N1B4_5660 0.70 +0.2 92,368 + Pf1N1B4_5660 0.77 +0.8 92,414 + Pf1N1B4_5660 0.79 +0.2 92,594 - +1.8 92,635 + -0.2 92,680 + +1.5 92,753 - +3.3 92,848 - +0.0 92,888 + -2.0 92,889 - +0.2 93,124 + +0.5 93,140 + +0.3 93,141 - +0.1 93,141 - -0.1 93,141 - +0.5 93,141 - -0.2 93,161 + +1.4 93,366 + +0.5 93,367 - +3.1 93,539 + Pf1N1B4_5661 0.13 -0.6 93,902 + Pf1N1B4_5661 0.23 -1.5 94,190 + Pf1N1B4_5661 0.31 +1.2 94,190 + Pf1N1B4_5661 0.31 +0.8 94,190 + Pf1N1B4_5661 0.31 +1.8 94,190 + Pf1N1B4_5661 0.31 -1.0 94,191 - Pf1N1B4_5661 0.31 -1.2 94,191 - Pf1N1B4_5661 0.31 +0.8 94,575 + Pf1N1B4_5661 0.41 +0.2 94,576 - Pf1N1B4_5661 0.41 -1.5 94,664 + Pf1N1B4_5661 0.44 +1.4 94,664 + Pf1N1B4_5661 0.44 +1.3 94,664 + +0.2 94,665 - -2.0 94,665 - +0.4 94,808 + Pf1N1B4_5661 0.48 -0.8 94,808 + Pf1N1B4_5661 0.48 +0.8 94,808 + Pf1N1B4_5661 0.48 +2.4 94,809 - Pf1N1B4_5661 0.48 +0.2 94,809 - Pf1N1B4_5661 0.48 +1.8 94,865 + Pf1N1B4_5661 0.49 -0.2 94,865 + +1.2 94,865 + Pf1N1B4_5661 0.49 +1.2 94,865 + Pf1N1B4_5661 0.49 +0.3 94,865 + Pf1N1B4_5661 0.49 +0.1 94,866 - Pf1N1B4_5661 0.49 +0.8 94,866 - Pf1N1B4_5661 0.49 +1.3 94,866 - Pf1N1B4_5661 0.49 -0.0 94,866 - Pf1N1B4_5661 0.49 -0.9 94,866 - Pf1N1B4_5661 0.49 +1.8 94,964 + Pf1N1B4_5661 0.52 +1.5 94,964 + Pf1N1B4_5661 0.52 -0.0 94,964 + Pf1N1B4_5661 0.52 +0.6 94,964 + Pf1N1B4_5661 0.52 +1.2 94,965 - Pf1N1B4_5661 0.52 +1.8 94,965 - Pf1N1B4_5661 0.52 +2.2 94,965 - Pf1N1B4_5661 0.52 +0.8 94,965 - Pf1N1B4_5661 0.52 +0.4 95,322 + Pf1N1B4_5661 0.61 +0.8 95,322 + Pf1N1B4_5661 0.61 +2.4 95,323 - Pf1N1B4_5661 0.61 -0.4 95,323 - +4.0 95,618 + Pf1N1B4_5661 0.69 -0.2 95,619 - Pf1N1B4_5661 0.69 -0.2 95,619 - +3.8 95,711 + Pf1N1B4_5661 0.72 +0.2 95,711 + Pf1N1B4_5661 0.72 +1.4 95,711 + Pf1N1B4_5661 0.72 +0.1 95,712 - +0.8 95,885 + Pf1N1B4_5661 0.77 +1.2 95,885 + Pf1N1B4_5661 0.77 +0.5 96,224 + Pf1N1B4_5661 0.86 +0.5 96,302 + Pf1N1B4_5661 0.88 +0.8 96,302 + Pf1N1B4_5661 0.88 +0.8 96,302 + Pf1N1B4_5661 0.88 +0.3 96,379 + Pf1N1B4_5661 0.90 +3.1 96,386 + +1.7 96,386 + -1.0 96,386 + +2.4 96,443 + +0.6 96,443 + -0.2 96,443 + +3.4 96,443 + +2.7 96,443 + +0.2 96,443 + -0.8 96,443 + +0.8 96,444 - +1.0 96,444 - +0.2 96,444 - +1.4 96,444 - -0.2 96,578 + +0.9 96,614 + -0.2 96,615 - +1.0 96,615 - +0.8 96,639 - +2.0 96,680 + +0.6 96,680 + +2.4 96,680 + +2.6 96,680 + +2.1 96,680 + +2.0 96,680 + +0.8 96,680 + +0.9 96,753 - -0.4 96,868 + +0.1 96,869 - -1.5 96,869 - +0.8 97,075 - Pf1N1B4_5662 0.74 -0.0 97,444 + Pf1N1B4_5663 0.30 -0.5 97,498 - Pf1N1B4_5663 0.36 -0.2
Or see this region's nucleotide sequence