Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5582 and Pf1N1B4_5583 are separated by 104 nucleotides Pf1N1B4_5583 and Pf1N1B4_5584 are separated by 11 nucleotides Pf1N1B4_5584 and Pf1N1B4_5585 are separated by 103 nucleotides
Pf1N1B4_5582 - Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25), at 2,599 to 5,022
_5582
Pf1N1B4_5583 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 5,127 to 5,732
_5583
Pf1N1B4_5584 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 5,744 to 6,463
_5584
Pf1N1B4_5585 - LysR family transcriptional regulator YdcI, at 6,567 to 7,496
_5585
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4.822 kb on + strand at 4.822 kb on + strand at 4.822 kb on + strand at 4.823 kb on - strand at 4.825 kb on + strand at 4.825 kb on + strand at 4.825 kb on + strand at 4.909 kb on + strand at 4.909 kb on + strand at 4.909 kb on + strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.910 kb on - strand at 4.936 kb on + strand at 4.937 kb on - strand at 4.937 kb on - strand at 4.937 kb on - strand at 4.987 kb on + strand at 4.987 kb on + strand at 4.988 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.166 kb on - strand at 5.170 kb on - strand at 5.170 kb on - strand at 5.170 kb on - strand at 5.563 kb on + strand at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.564 kb on - strand, within Pf1N1B4_5583 at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.679 kb on + strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.680 kb on - strand at 5.683 kb on + strand at 5.684 kb on - strand at 5.684 kb on - strand at 5.925 kb on + strand, within Pf1N1B4_5584 at 6.051 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.054 kb on + strand, within Pf1N1B4_5584 at 6.179 kb on - strand, within Pf1N1B4_5584 at 6.390 kb on + strand, within Pf1N1B4_5584 at 6.421 kb on + strand at 6.788 kb on - strand, within Pf1N1B4_5585 at 6.995 kb on - strand, within Pf1N1B4_5585 at 6.995 kb on - strand, within Pf1N1B4_5585 at 6.995 kb on - strand, within Pf1N1B4_5585 at 7.153 kb on - strand, within Pf1N1B4_5585 at 7.153 kb on - strand, within Pf1N1B4_5585 at 7.332 kb on + strand, within Pf1N1B4_5585 at 7.333 kb on - strand, within Pf1N1B4_5585 at 7.334 kb on + strand, within Pf1N1B4_5585 at 7.370 kb on + strand, within Pf1N1B4_5585
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,822 + +0.5 4,822 + +0.4 4,822 + -1.4 4,823 - +2.0 4,825 + +0.4 4,825 + -1.2 4,825 + +4.0 4,909 + -1.8 4,909 + +0.3 4,909 + +0.6 4,910 - +1.4 4,910 - -2.0 4,910 - +0.8 4,910 - +1.2 4,910 - -1.5 4,910 - -0.5 4,910 - +0.8 4,910 - -0.8 4,936 + -0.0 4,937 - -0.2 4,937 - +0.8 4,937 - +0.4 4,987 + +0.2 4,987 + +0.4 4,988 - +0.8 5,166 - -0.2 5,166 - +0.2 5,166 - -0.3 5,166 - +1.1 5,170 - -0.8 5,170 - +0.8 5,170 - -1.2 5,563 + -1.8 5,564 - Pf1N1B4_5583 0.72 +0.0 5,564 - Pf1N1B4_5583 0.72 -1.5 5,564 - Pf1N1B4_5583 0.72 +1.4 5,679 + +0.8 5,679 + -0.2 5,679 + -0.2 5,679 + -0.2 5,679 + -0.8 5,679 + -0.5 5,679 + -0.8 5,679 + +0.4 5,680 - +0.2 5,680 - +2.8 5,680 - -0.2 5,680 - +2.2 5,680 - +1.2 5,680 - +1.4 5,680 - +1.2 5,680 - -0.1 5,680 - +0.4 5,680 - -0.2 5,680 - +0.5 5,680 - +0.8 5,683 + +1.1 5,684 - -0.3 5,684 - +1.8 5,925 + Pf1N1B4_5584 0.25 +1.2 6,051 + Pf1N1B4_5584 0.43 -0.2 6,054 + Pf1N1B4_5584 0.43 +0.2 6,054 + Pf1N1B4_5584 0.43 -1.2 6,054 + Pf1N1B4_5584 0.43 +1.8 6,179 - Pf1N1B4_5584 0.60 +1.6 6,390 + Pf1N1B4_5584 0.90 -1.8 6,421 + +0.2 6,788 - Pf1N1B4_5585 0.24 -0.4 6,995 - Pf1N1B4_5585 0.46 +0.6 6,995 - Pf1N1B4_5585 0.46 +0.9 6,995 - Pf1N1B4_5585 0.46 -1.4 7,153 - Pf1N1B4_5585 0.63 -0.5 7,153 - Pf1N1B4_5585 0.63 +0.6 7,332 + Pf1N1B4_5585 0.82 +0.3 7,333 - Pf1N1B4_5585 0.82 +0.6 7,334 + Pf1N1B4_5585 0.82 -1.8 7,370 + Pf1N1B4_5585 0.86 -0.6
Or see this region's nucleotide sequence