Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5546 and Pf1N1B4_5547 are separated by 37 nucleotides Pf1N1B4_5547 and Pf1N1B4_5548 are separated by 150 nucleotides Pf1N1B4_5548 and Pf1N1B4_5549 are separated by 989 nucleotides
Pf1N1B4_5546 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 317,519 to 318,460
_5546
Pf1N1B4_5547 - hypothetical protein, at 318,498 to 318,779
_5547
Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943
_5548
Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061
_5549
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 317.940 kb on + strand, within Pf1N1B4_5546 at 317.948 kb on + strand, within Pf1N1B4_5546 at 317.948 kb on + strand, within Pf1N1B4_5546 at 317.985 kb on + strand, within Pf1N1B4_5546 at 317.998 kb on + strand, within Pf1N1B4_5546 at 317.998 kb on + strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 318.105 kb on + strand, within Pf1N1B4_5546 at 318.130 kb on + strand, within Pf1N1B4_5546 at 318.131 kb on - strand, within Pf1N1B4_5546 at 318.167 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.250 kb on - strand, within Pf1N1B4_5546 at 318.253 kb on + strand at 318.253 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.316 kb on - strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.432 kb on + strand at 318.440 kb on + strand at 318.610 kb on - strand, within Pf1N1B4_5547 at 318.651 kb on - strand, within Pf1N1B4_5547 at 318.896 kb on + strand at 318.897 kb on - strand at 318.920 kb on + strand at 318.986 kb on - strand at 318.986 kb on - strand at 318.986 kb on - strand at 318.986 kb on - strand at 319.027 kb on + strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.044 kb on + strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.182 kb on + strand, within Pf1N1B4_5548 at 319.360 kb on - strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.502 kb on + strand, within Pf1N1B4_5548 at 319.503 kb on - strand, within Pf1N1B4_5548 at 319.503 kb on - strand, within Pf1N1B4_5548 at 319.596 kb on + strand, within Pf1N1B4_5548 at 319.611 kb on + strand, within Pf1N1B4_5548 at 319.611 kb on + strand, within Pf1N1B4_5548 at 319.612 kb on - strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.628 kb on - strand, within Pf1N1B4_5548 at 319.716 kb on + strand, within Pf1N1B4_5548 at 319.730 kb on - strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.780 kb on - strand, within Pf1N1B4_5548 at 319.858 kb on + strand at 319.858 kb on + strand at 319.859 kb on - strand at 319.925 kb on - strand at 319.925 kb on - strand at 319.925 kb on - strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.947 kb on + strand at 319.948 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.966 kb on + strand at 319.971 kb on + strand at 319.975 kb on - strand at 319.975 kb on - strand at 320.021 kb on + strand at 320.022 kb on - strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.174 kb on + strand at 320.174 kb on + strand at 320.175 kb on - strand at 320.175 kb on - strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.179 kb on - strand at 320.179 kb on - strand at 320.200 kb on - strand at 320.255 kb on + strand at 320.256 kb on - strand at 320.277 kb on - strand at 320.282 kb on - strand at 320.339 kb on + strand at 320.339 kb on + strand at 320.340 kb on - strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.367 kb on + strand at 320.367 kb on + strand at 320.368 kb on - strand at 320.409 kb on - strand at 320.558 kb on + strand at 320.558 kb on + strand at 320.559 kb on - strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.681 kb on + strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.735 kb on - strand at 320.787 kb on + strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.848 kb on + strand at 320.849 kb on - strand at 320.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 317,940 + Pf1N1B4_5546 0.45 +0.2 317,948 + Pf1N1B4_5546 0.46 -0.6 317,948 + Pf1N1B4_5546 0.46 -1.1 317,985 + Pf1N1B4_5546 0.49 +0.6 317,998 + Pf1N1B4_5546 0.51 -0.9 317,998 + Pf1N1B4_5546 0.51 +0.1 317,999 - Pf1N1B4_5546 0.51 +0.9 317,999 - Pf1N1B4_5546 0.51 -1.6 317,999 - Pf1N1B4_5546 0.51 +0.3 318,105 + Pf1N1B4_5546 0.62 -0.4 318,130 + Pf1N1B4_5546 0.65 +0.6 318,131 - Pf1N1B4_5546 0.65 -0.9 318,167 + Pf1N1B4_5546 0.69 -1.6 318,249 + Pf1N1B4_5546 0.77 +0.6 318,249 + Pf1N1B4_5546 0.77 -1.9 318,249 + Pf1N1B4_5546 0.77 -1.1 318,249 + Pf1N1B4_5546 0.77 -0.6 318,250 - Pf1N1B4_5546 0.78 +1.2 318,253 + +2.8 318,253 + Pf1N1B4_5546 0.78 +2.6 318,315 + Pf1N1B4_5546 0.85 +0.4 318,315 + Pf1N1B4_5546 0.85 -1.6 318,315 + Pf1N1B4_5546 0.85 -1.4 318,315 + Pf1N1B4_5546 0.85 +1.8 318,316 - Pf1N1B4_5546 0.85 -1.4 318,358 + Pf1N1B4_5546 0.89 +1.0 318,358 + Pf1N1B4_5546 0.89 -0.4 318,358 + Pf1N1B4_5546 0.89 +0.2 318,358 + Pf1N1B4_5546 0.89 +1.2 318,358 + Pf1N1B4_5546 0.89 -0.8 318,359 - Pf1N1B4_5546 0.89 +0.3 318,359 - Pf1N1B4_5546 0.89 +2.0 318,359 - Pf1N1B4_5546 0.89 -1.4 318,359 - Pf1N1B4_5546 0.89 +0.9 318,432 + -1.1 318,440 + -0.4 318,610 - Pf1N1B4_5547 0.40 +0.9 318,651 - Pf1N1B4_5547 0.54 +1.5 318,896 + +0.5 318,897 - +0.5 318,920 + +0.1 318,986 - -1.8 318,986 - +0.8 318,986 - +0.5 318,986 - -1.8 319,027 + +3.2 319,028 - +0.6 319,028 - +0.4 319,028 - -0.2 319,028 - -1.1 319,044 + Pf1N1B4_5548 0.11 +0.4 319,060 - Pf1N1B4_5548 0.13 -0.1 319,060 - Pf1N1B4_5548 0.13 +0.6 319,060 - Pf1N1B4_5548 0.13 -0.2 319,182 + Pf1N1B4_5548 0.25 +0.6 319,360 - Pf1N1B4_5548 0.42 -0.1 319,408 + Pf1N1B4_5548 0.47 +0.4 319,408 + Pf1N1B4_5548 0.47 +0.9 319,408 + Pf1N1B4_5548 0.47 +0.2 319,408 + Pf1N1B4_5548 0.47 +0.8 319,502 + Pf1N1B4_5548 0.56 -1.6 319,503 - Pf1N1B4_5548 0.57 +0.9 319,503 - Pf1N1B4_5548 0.57 +2.6 319,596 + Pf1N1B4_5548 0.66 +0.5 319,611 + Pf1N1B4_5548 0.67 -0.4 319,611 + Pf1N1B4_5548 0.67 +0.3 319,612 - Pf1N1B4_5548 0.67 -0.1 319,627 + Pf1N1B4_5548 0.69 -0.8 319,627 + Pf1N1B4_5548 0.69 +1.8 319,627 + Pf1N1B4_5548 0.69 -1.1 319,627 + Pf1N1B4_5548 0.69 +0.8 319,628 - Pf1N1B4_5548 0.69 +0.2 319,716 + Pf1N1B4_5548 0.78 +1.2 319,730 - Pf1N1B4_5548 0.79 +0.2 319,779 + Pf1N1B4_5548 0.84 +1.0 319,779 + Pf1N1B4_5548 0.84 +0.8 319,779 + Pf1N1B4_5548 0.84 +0.0 319,779 + Pf1N1B4_5548 0.84 +0.6 319,780 - Pf1N1B4_5548 0.84 +0.8 319,858 + -0.4 319,858 + +1.2 319,859 - +0.8 319,925 - +0.1 319,925 - +0.6 319,925 - +0.0 319,926 + +0.9 319,926 + +0.6 319,926 + +2.0 319,926 + +0.6 319,926 + -0.8 319,926 + +1.2 319,926 + +1.6 319,926 + -0.8 319,927 - +0.2 319,927 - +0.2 319,927 - +2.1 319,927 - -1.6 319,927 - +0.7 319,947 + +0.1 319,948 - -0.9 319,954 - -0.4 319,954 - -1.6 319,954 - +0.9 319,954 - -1.9 319,966 + +1.1 319,971 + -1.4 319,975 - +1.1 319,975 - +1.0 320,021 + -0.4 320,022 - +2.0 320,135 + -0.8 320,135 + -1.6 320,135 + -0.0 320,135 + +1.1 320,135 + +0.2 320,135 + -1.4 320,135 + +0.1 320,136 - +0.8 320,136 - -0.4 320,136 - +2.0 320,136 - -1.6 320,136 - +0.7 320,174 + +0.2 320,174 + +0.5 320,175 - -0.5 320,175 - +1.6 320,178 + +1.6 320,178 + -0.4 320,178 + -0.4 320,178 + +0.8 320,178 + -0.4 320,179 - +0.5 320,179 - +1.8 320,200 - +0.1 320,255 + -0.9 320,256 - +0.0 320,277 - +0.3 320,282 - +1.4 320,339 + +1.8 320,339 + -0.6 320,340 - -2.4 320,365 + +0.2 320,365 + +0.2 320,365 + +1.2 320,366 - -0.7 320,366 - +0.2 320,366 - +0.2 320,366 - -0.9 320,366 - -1.4 320,367 + -1.4 320,367 + -0.1 320,368 - -0.1 320,409 - +0.0 320,558 + -0.4 320,558 + +0.2 320,559 - -0.6 320,622 + -1.1 320,622 + +0.5 320,622 + +1.2 320,623 - +1.2 320,623 - -0.1 320,623 - +1.2 320,681 + -1.4 320,682 - +2.2 320,682 - +1.2 320,682 - -0.7 320,685 + -0.1 320,685 + -0.2 320,685 + +0.8 320,685 + +3.4 320,685 + -0.6 320,685 + +0.1 320,686 - -0.8 320,686 - -0.9 320,686 - +0.6 320,686 - +0.1 320,686 - +0.2 320,686 - +1.1 320,735 - +1.2 320,787 + -1.3 320,788 - -0.0 320,788 - -0.4 320,848 + +2.8 320,849 - -0.9 320,935 - +1.8
Or see this region's nucleotide sequence