Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5328 and Pf1N1B4_5329 are separated by 219 nucleotides Pf1N1B4_5329 and Pf1N1B4_5330 are separated by 25 nucleotides Pf1N1B4_5330 and Pf1N1B4_5331 are separated by 356 nucleotides Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides
Pf1N1B4_5328 - hypothetical protein, at 76,949 to 77,134
_5328
Pf1N1B4_5329 - hypothetical protein, at 77,354 to 77,896
_5329
Pf1N1B4_5330 - hypothetical protein, at 77,922 to 78,233
_5330
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 76.946 kb on - strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.951 kb on - strand at 76.951 kb on - strand at 76.954 kb on + strand at 76.954 kb on + strand at 76.955 kb on - strand at 76.955 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.204 kb on - strand at 77.249 kb on + strand at 77.249 kb on + strand at 77.254 kb on + strand at 77.255 kb on - strand at 77.255 kb on - strand at 77.336 kb on + strand at 77.336 kb on + strand at 77.337 kb on - strand at 77.362 kb on + strand at 77.371 kb on + strand at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.411 kb on - strand, within Pf1N1B4_5329 at 77.686 kb on + strand, within Pf1N1B4_5329 at 77.752 kb on - strand, within Pf1N1B4_5329 at 77.781 kb on + strand, within Pf1N1B4_5329 at 77.781 kb on + strand at 77.782 kb on - strand, within Pf1N1B4_5329 at 77.847 kb on + strand at 77.923 kb on + strand at 77.923 kb on + strand at 77.980 kb on + strand, within Pf1N1B4_5330 at 78.022 kb on - strand, within Pf1N1B4_5330 at 78.152 kb on + strand at 78.152 kb on + strand, within Pf1N1B4_5330 at 78.153 kb on - strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.314 kb on - strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.392 kb on + strand at 78.392 kb on + strand at 78.425 kb on + strand at 78.425 kb on + strand at 78.426 kb on - strand at 78.426 kb on - strand at 78.474 kb on + strand at 78.474 kb on + strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 76,946 - -0.6 76,950 + +1.5 76,950 + +0.9 76,950 + +1.8 76,951 - +0.9 76,951 - -0.3 76,954 + +1.2 76,954 + +0.7 76,955 - +1.2 76,955 - +1.2 77,161 - +1.2 77,161 - -0.3 77,161 - +1.6 77,161 - +0.7 77,204 - +1.7 77,249 + +1.2 77,249 + +1.2 77,254 + -0.1 77,255 - +0.5 77,255 - -0.8 77,336 + +0.0 77,336 + -1.2 77,337 - -1.6 77,362 + +1.4 77,371 + +1.1 77,410 + Pf1N1B4_5329 0.10 +1.2 77,410 + Pf1N1B4_5329 0.10 -0.1 77,410 + Pf1N1B4_5329 0.10 -1.4 77,410 + Pf1N1B4_5329 0.10 -0.3 77,411 - Pf1N1B4_5329 0.10 +0.1 77,686 + Pf1N1B4_5329 0.61 +1.1 77,752 - Pf1N1B4_5329 0.73 +0.2 77,781 + Pf1N1B4_5329 0.79 +1.1 77,781 + +0.2 77,782 - Pf1N1B4_5329 0.79 -2.2 77,847 + -0.2 77,923 + -0.2 77,923 + +0.9 77,980 + Pf1N1B4_5330 0.19 -1.0 78,022 - Pf1N1B4_5330 0.32 -1.8 78,152 + +0.2 78,152 + Pf1N1B4_5330 0.74 +0.7 78,153 - +1.2 78,313 + -0.5 78,313 + +1.2 78,313 + -1.1 78,313 + +0.4 78,314 - -0.3 78,336 + -0.1 78,336 + +0.0 78,336 + +0.7 78,336 + +0.7 78,351 + +1.2 78,351 + +1.8 78,351 + +0.2 78,352 - -1.6 78,352 - +0.8 78,392 + -1.8 78,392 + -0.8 78,425 + -0.5 78,425 + +0.8 78,426 - +1.4 78,426 - -0.5 78,474 + +2.2 78,474 + +1.6 78,475 - -2.1 78,475 - +0.5 78,475 - +2.2 78,475 - -0.8 78,475 - +0.8 78,597 - -0.2 78,597 - +0.7 78,597 - +0.1 78,729 + Pf1N1B4_5331 0.81 -0.3 78,730 - Pf1N1B4_5331 0.82 -2.6 78,730 - Pf1N1B4_5331 0.82 +1.1 78,730 - Pf1N1B4_5331 0.82 +0.2 78,739 - Pf1N1B4_5331 0.87 +0.4 78,971 - +1.2 79,045 + Pf1N1B4_5333 0.12 -0.8 79,045 + Pf1N1B4_5333 0.12 -1.3 79,046 - Pf1N1B4_5333 0.12 +1.2 79,046 - Pf1N1B4_5333 0.12 -1.4 79,046 - Pf1N1B4_5333 0.12 -1.2 79,106 + Pf1N1B4_5333 0.22 -1.5
Or see this region's nucleotide sequence