Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5015 and Pf1N1B4_5016 are separated by 280 nucleotides Pf1N1B4_5016 and Pf1N1B4_5017 are separated by 140 nucleotides Pf1N1B4_5017 and Pf1N1B4_5018 are separated by 177 nucleotides Pf1N1B4_5018 and Pf1N1B4_5019 are separated by 72 nucleotides
Pf1N1B4_5015 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 5,566,595 to 5,567,434
_5015
Pf1N1B4_5016 - hypothetical protein, at 5,567,715 to 5,567,903
_5016
Pf1N1B4_5017 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,568,044 to 5,568,325
_5017
Pf1N1B4_5018 - FIG00962345: hypothetical protein, at 5,568,503 to 5,568,733
_5018
Pf1N1B4_5019 - FIG00958773: hypothetical protein, at 5,568,806 to 5,569,162
_5019
Position (kb) 5568
5569 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.226 kb on + strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.437 kb on - strand at 5567.437 kb on - strand at 5567.437 kb on - strand at 5567.478 kb on + strand at 5567.498 kb on + strand at 5567.498 kb on + strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.533 kb on - strand at 5567.552 kb on + strand at 5567.553 kb on - strand at 5567.581 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.651 kb on - strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.955 kb on + strand at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.238 kb on + strand, within Pf1N1B4_5017 at 5568.291 kb on - strand, within Pf1N1B4_5017 at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.324 kb on - strand at 5568.342 kb on - strand at 5568.361 kb on - strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.505 kb on - strand at 5568.505 kb on - strand at 5568.508 kb on + strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.692 kb on - strand at 5568.692 kb on - strand, within Pf1N1B4_5018 at 5568.804 kb on + strand at 5568.805 kb on - strand at 5568.805 kb on - strand at 5568.839 kb on + strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.924 kb on - strand, within Pf1N1B4_5019 at 5569.064 kb on + strand, within Pf1N1B4_5019 at 5569.229 kb on - strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 5,567,140 - Pf1N1B4_5015 0.65 -1.4 5,567,140 - Pf1N1B4_5015 0.65 +2.0 5,567,185 - Pf1N1B4_5015 0.70 -0.4 5,567,185 - Pf1N1B4_5015 0.70 +0.3 5,567,226 + Pf1N1B4_5015 0.75 +0.2 5,567,227 - Pf1N1B4_5015 0.75 +0.8 5,567,227 - Pf1N1B4_5015 0.75 +1.6 5,567,434 + -0.5 5,567,434 + +0.2 5,567,434 + +3.2 5,567,436 + -1.8 5,567,436 + +0.6 5,567,436 + +0.2 5,567,437 - -0.0 5,567,437 - -0.0 5,567,437 - -0.1 5,567,478 + +0.6 5,567,498 + +0.6 5,567,498 + -0.4 5,567,499 - +0.7 5,567,499 - +2.2 5,567,499 - +2.3 5,567,499 - -0.5 5,567,499 - +0.6 5,567,533 - -1.0 5,567,552 + +0.4 5,567,553 - -0.5 5,567,581 + +0.4 5,567,583 + +1.0 5,567,583 + -0.4 5,567,583 + +1.4 5,567,584 - -0.6 5,567,584 - +0.6 5,567,584 - +0.3 5,567,651 - -0.7 5,567,712 + +1.6 5,567,712 + +0.6 5,567,712 + +1.2 5,567,713 - -0.0 5,567,713 - -2.2 5,567,713 - -0.2 5,567,713 - -1.4 5,567,955 + +0.2 5,568,110 + Pf1N1B4_5017 0.23 -1.4 5,568,110 + Pf1N1B4_5017 0.23 -0.7 5,568,238 + Pf1N1B4_5017 0.69 +0.2 5,568,291 - Pf1N1B4_5017 0.88 -0.0 5,568,323 + +0.6 5,568,323 + +0.1 5,568,323 + +0.6 5,568,323 + -0.4 5,568,324 - +2.2 5,568,342 - +0.2 5,568,361 - +0.9 5,568,504 + +0.9 5,568,504 + -0.2 5,568,504 + -0.1 5,568,505 - -0.8 5,568,505 - +0.1 5,568,508 + -0.0 5,568,509 - -0.4 5,568,509 - +0.6 5,568,509 - +1.4 5,568,691 + Pf1N1B4_5018 0.81 +1.2 5,568,691 + Pf1N1B4_5018 0.81 -0.6 5,568,691 + Pf1N1B4_5018 0.81 +0.3 5,568,691 + Pf1N1B4_5018 0.81 -1.0 5,568,691 + Pf1N1B4_5018 0.81 +0.7 5,568,691 + Pf1N1B4_5018 0.81 -0.8 5,568,692 - -0.0 5,568,692 - Pf1N1B4_5018 0.82 -0.0 5,568,804 + -0.0 5,568,805 - +0.1 5,568,805 - -1.0 5,568,839 + +0.8 5,568,840 - +0.2 5,568,840 - -0.0 5,568,840 - +0.5 5,568,840 - +0.2 5,568,840 - -0.0 5,568,924 - Pf1N1B4_5019 0.33 -0.2 5,569,064 + Pf1N1B4_5019 0.72 -0.4 5,569,229 - +2.6 5,569,237 + +1.0 5,569,237 + +1.8 5,569,237 + +2.0 5,569,250 - +1.6
Or see this region's nucleotide sequence