Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4976 and Pf1N1B4_4977 are separated by 111 nucleotides Pf1N1B4_4977 and Pf1N1B4_4978 are separated by 51 nucleotides
Pf1N1B4_4976 - D-serine/D-alanine/glycine transporter, at 5,515,291 to 5,516,712
_4976
Pf1N1B4_4977 - Ornithine cyclodeaminase (EC 4.3.1.12), at 5,516,824 to 5,517,849
_4977
Pf1N1B4_4978 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 5,517,901 to 5,518,935
_4978
Position (kb) 5516
5517
5518 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5516.038 kb on + strand, within Pf1N1B4_4976 at 5516.409 kb on - strand, within Pf1N1B4_4976 at 5516.492 kb on - strand, within Pf1N1B4_4976 at 5516.547 kb on + strand at 5516.549 kb on + strand, within Pf1N1B4_4976 at 5516.554 kb on - strand, within Pf1N1B4_4976 at 5516.557 kb on + strand, within Pf1N1B4_4976 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.186 kb on + strand, within Pf1N1B4_4977 at 5517.320 kb on + strand at 5517.321 kb on - strand, within Pf1N1B4_4977 at 5517.641 kb on + strand at 5517.641 kb on + strand, within Pf1N1B4_4977 at 5517.642 kb on - strand, within Pf1N1B4_4977 at 5517.654 kb on - strand at 5517.727 kb on - strand, within Pf1N1B4_4977 at 5517.968 kb on + strand at 5518.102 kb on - strand, within Pf1N1B4_4978 at 5518.106 kb on - strand, within Pf1N1B4_4978 at 5518.163 kb on + strand, within Pf1N1B4_4978 at 5518.208 kb on + strand, within Pf1N1B4_4978 at 5518.253 kb on + strand at 5518.256 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.377 kb on + strand, within Pf1N1B4_4978 at 5518.427 kb on + strand at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.739 kb on + strand, within Pf1N1B4_4978
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 5,516,038 + Pf1N1B4_4976 0.53 -1.4 5,516,409 - Pf1N1B4_4976 0.79 -2.4 5,516,492 - Pf1N1B4_4976 0.84 -1.3 5,516,547 + -0.0 5,516,549 + Pf1N1B4_4976 0.88 -0.8 5,516,554 - Pf1N1B4_4976 0.89 +0.6 5,516,557 + Pf1N1B4_4976 0.89 -0.2 5,517,038 + Pf1N1B4_4977 0.21 -0.8 5,517,038 + Pf1N1B4_4977 0.21 -0.6 5,517,186 + Pf1N1B4_4977 0.35 -0.0 5,517,320 + -1.4 5,517,321 - Pf1N1B4_4977 0.48 -0.4 5,517,641 + +1.1 5,517,641 + Pf1N1B4_4977 0.80 +0.0 5,517,642 - Pf1N1B4_4977 0.80 -3.0 5,517,654 - +0.6 5,517,727 - Pf1N1B4_4977 0.88 +1.9 5,517,968 + +1.2 5,518,102 - Pf1N1B4_4978 0.19 +0.4 5,518,106 - Pf1N1B4_4978 0.20 +1.0 5,518,163 + Pf1N1B4_4978 0.25 -0.0 5,518,208 + Pf1N1B4_4978 0.30 +0.2 5,518,253 + +1.6 5,518,256 + Pf1N1B4_4978 0.34 -0.7 5,518,316 + Pf1N1B4_4978 0.40 -0.1 5,518,316 + Pf1N1B4_4978 0.40 +1.3 5,518,377 + Pf1N1B4_4978 0.46 +0.4 5,518,427 + +0.6 5,518,445 + Pf1N1B4_4978 0.53 +1.2 5,518,445 + Pf1N1B4_4978 0.53 +0.6 5,518,445 + Pf1N1B4_4978 0.53 +0.2 5,518,445 + Pf1N1B4_4978 0.53 +1.6 5,518,739 + Pf1N1B4_4978 0.81 -0.8
Or see this region's nucleotide sequence