Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4950 and Pf1N1B4_4951 are separated by 140 nucleotides Pf1N1B4_4951 and Pf1N1B4_4952 are separated by 566 nucleotides
Pf1N1B4_4950 - formate dehydrogenase formation protein FdhE, at 5,486,644 to 5,487,570
_4950
Pf1N1B4_4951 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 5,487,711 to 5,488,073
_4951
Pf1N1B4_4952 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 5,488,640 to 5,490,070
_4952
Position (kb) 5487
5488
5489 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.319 kb on + strand, within Pf1N1B4_4950 at 5487.346 kb on + strand, within Pf1N1B4_4950 at 5487.347 kb on - strand, within Pf1N1B4_4950 at 5487.425 kb on - strand, within Pf1N1B4_4950 at 5487.580 kb on + strand at 5487.627 kb on + strand at 5487.712 kb on + strand at 5487.713 kb on - strand at 5487.713 kb on - strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.786 kb on + strand, within Pf1N1B4_4951 at 5487.787 kb on - strand at 5487.794 kb on + strand at 5487.813 kb on + strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.968 kb on - strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5488.073 kb on + strand at 5488.073 kb on + strand at 5488.074 kb on - strand at 5488.074 kb on - strand at 5488.076 kb on + strand at 5488.226 kb on - strand at 5488.363 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.396 kb on - strand at 5488.396 kb on - strand at 5488.412 kb on - strand at 5488.585 kb on + strand at 5488.834 kb on - strand, within Pf1N1B4_4952
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 5,486,783 - Pf1N1B4_4950 0.15 -0.8 5,486,783 - Pf1N1B4_4950 0.15 -2.0 5,487,247 + Pf1N1B4_4950 0.65 +0.9 5,487,247 + Pf1N1B4_4950 0.65 +1.6 5,487,319 + Pf1N1B4_4950 0.73 +0.6 5,487,346 + Pf1N1B4_4950 0.76 -0.1 5,487,347 - Pf1N1B4_4950 0.76 -1.0 5,487,425 - Pf1N1B4_4950 0.84 +0.2 5,487,580 + -0.4 5,487,627 + -0.3 5,487,712 + -1.0 5,487,713 - -0.8 5,487,713 - -1.0 5,487,716 + +0.3 5,487,716 + +0.6 5,487,716 + -2.2 5,487,716 + +0.6 5,487,716 + +0.2 5,487,716 + -0.1 5,487,717 - -0.3 5,487,717 - +1.0 5,487,717 - -2.2 5,487,786 + Pf1N1B4_4951 0.21 +1.3 5,487,787 - -1.0 5,487,794 + +0.4 5,487,813 + Pf1N1B4_4951 0.28 +2.6 5,487,814 - Pf1N1B4_4951 0.28 +0.6 5,487,814 - Pf1N1B4_4951 0.28 -1.0 5,487,968 - Pf1N1B4_4951 0.71 -0.3 5,487,970 + Pf1N1B4_4951 0.71 +0.4 5,487,970 + Pf1N1B4_4951 0.71 +1.9 5,488,073 + +0.4 5,488,073 + +3.2 5,488,074 - -0.6 5,488,074 - -2.6 5,488,076 + +1.0 5,488,226 - -0.3 5,488,363 + +1.2 5,488,395 + -0.3 5,488,395 + -2.4 5,488,395 + -0.0 5,488,396 - -0.0 5,488,396 - +1.7 5,488,412 - +1.4 5,488,585 + -1.0 5,488,834 - Pf1N1B4_4952 0.14 -1.4
Or see this region's nucleotide sequence