Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4947 and Pf1N1B4_4948 are separated by 0 nucleotides Pf1N1B4_4948 and Pf1N1B4_4949 overlap by 4 nucleotides Pf1N1B4_4949 and Pf1N1B4_4950 are separated by 4 nucleotides
Pf1N1B4_4947 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 5,481,979 to 5,485,044
_4947
Pf1N1B4_4948 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 5,485,045 to 5,485,989
_4948
Pf1N1B4_4949 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 5,485,986 to 5,486,639
_4949
Pf1N1B4_4950 - formate dehydrogenase formation protein FdhE, at 5,486,644 to 5,487,570
_4950
Position (kb) 5485
5486
5487 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5485.043 kb on - strand at 5485.226 kb on - strand, within Pf1N1B4_4948 at 5485.254 kb on + strand at 5485.254 kb on + strand at 5485.504 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.629 kb on + strand, within Pf1N1B4_4948 at 5485.670 kb on + strand, within Pf1N1B4_4948 at 5485.671 kb on - strand, within Pf1N1B4_4948 at 5485.672 kb on + strand, within Pf1N1B4_4948 at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.673 kb on - strand at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.709 kb on - strand at 5485.709 kb on - strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.858 kb on + strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5486.011 kb on - strand at 5486.011 kb on - strand at 5486.157 kb on + strand at 5486.157 kb on + strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.307 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.672 kb on + strand at 5486.672 kb on + strand at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.319 kb on + strand, within Pf1N1B4_4950 at 5487.346 kb on + strand, within Pf1N1B4_4950 at 5487.347 kb on - strand, within Pf1N1B4_4950 at 5487.425 kb on - strand, within Pf1N1B4_4950 at 5487.580 kb on + strand at 5487.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 5,485,043 - +2.2 5,485,226 - Pf1N1B4_4948 0.19 -1.9 5,485,254 + +1.6 5,485,254 + +0.6 5,485,504 + Pf1N1B4_4948 0.49 -1.0 5,485,506 + Pf1N1B4_4948 0.49 +0.6 5,485,506 + Pf1N1B4_4948 0.49 +0.7 5,485,506 + Pf1N1B4_4948 0.49 -0.7 5,485,507 - Pf1N1B4_4948 0.49 -0.4 5,485,507 - Pf1N1B4_4948 0.49 +0.8 5,485,507 - Pf1N1B4_4948 0.49 +0.4 5,485,629 + Pf1N1B4_4948 0.62 +0.6 5,485,670 + Pf1N1B4_4948 0.66 +0.6 5,485,671 - Pf1N1B4_4948 0.66 -0.4 5,485,672 + Pf1N1B4_4948 0.66 +3.6 5,485,673 - Pf1N1B4_4948 0.66 +0.6 5,485,673 - -0.4 5,485,673 - Pf1N1B4_4948 0.66 -0.2 5,485,708 + Pf1N1B4_4948 0.70 +0.9 5,485,708 + Pf1N1B4_4948 0.70 +0.8 5,485,708 + Pf1N1B4_4948 0.70 +1.8 5,485,708 + Pf1N1B4_4948 0.70 +0.6 5,485,708 + Pf1N1B4_4948 0.70 +1.1 5,485,708 + +0.3 5,485,709 - Pf1N1B4_4948 0.70 +0.9 5,485,709 - -0.4 5,485,709 - +1.6 5,485,709 - Pf1N1B4_4948 0.70 +0.6 5,485,858 + Pf1N1B4_4948 0.86 +0.0 5,485,859 - Pf1N1B4_4948 0.86 +0.9 5,485,859 - Pf1N1B4_4948 0.86 -0.4 5,485,859 - Pf1N1B4_4948 0.86 -0.7 5,486,011 - +0.6 5,486,011 - +0.9 5,486,157 + -0.0 5,486,157 + Pf1N1B4_4949 0.26 -1.0 5,486,158 - Pf1N1B4_4949 0.26 +1.2 5,486,158 - Pf1N1B4_4949 0.26 -1.0 5,486,221 - Pf1N1B4_4949 0.36 -1.7 5,486,221 - Pf1N1B4_4949 0.36 -2.9 5,486,221 - Pf1N1B4_4949 0.36 -0.4 5,486,307 + Pf1N1B4_4949 0.49 +0.6 5,486,493 + Pf1N1B4_4949 0.78 -0.4 5,486,493 + Pf1N1B4_4949 0.78 -1.2 5,486,493 + Pf1N1B4_4949 0.78 -1.9 5,486,672 + +0.6 5,486,672 + +0.6 5,486,783 - Pf1N1B4_4950 0.15 -0.8 5,486,783 - Pf1N1B4_4950 0.15 -2.0 5,487,247 + Pf1N1B4_4950 0.65 +0.9 5,487,247 + Pf1N1B4_4950 0.65 +1.6 5,487,319 + Pf1N1B4_4950 0.73 +0.6 5,487,346 + Pf1N1B4_4950 0.76 -0.1 5,487,347 - Pf1N1B4_4950 0.76 -1.0 5,487,425 - Pf1N1B4_4950 0.84 +0.2 5,487,580 + -0.4 5,487,627 + -0.3
Or see this region's nucleotide sequence