Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4912

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4910 and Pf1N1B4_4911 are separated by 95 nucleotidesPf1N1B4_4911 and Pf1N1B4_4912 are separated by 45 nucleotidesPf1N1B4_4912 and Pf1N1B4_4913 are separated by 448 nucleotides Pf1N1B4_4910 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 5,440,060 to 5,441,352 _4910 Pf1N1B4_4911 - FIG00953249: hypothetical protein, at 5,441,448 to 5,442,104 _4911 Pf1N1B4_4912 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,442,150 to 5,442,638 _4912 Pf1N1B4_4913 - hypothetical protein, at 5,443,087 to 5,443,866 _4913 Position (kb) 5442 5443Strain fitness (log2 ratio) -2 -1 0 1at 5441.402 kb on - strandat 5441.402 kb on - strandat 5441.441 kb on - strandat 5441.441 kb on - strandat 5441.441 kb on - strandat 5442.045 kb on + strandat 5442.125 kb on - strandat 5442.125 kb on - strandat 5442.161 kb on - strandat 5442.164 kb on - strandat 5442.303 kb on + strand, within Pf1N1B4_4912at 5442.306 kb on - strand, within Pf1N1B4_4912at 5442.316 kb on - strandat 5442.362 kb on - strand, within Pf1N1B4_4912at 5442.443 kb on - strand, within Pf1N1B4_4912at 5442.565 kb on - strand, within Pf1N1B4_4912at 5442.963 kb on + strandat 5443.008 kb on + strandat 5443.009 kb on - strandat 5443.009 kb on - strandat 5443.009 kb on - strandat 5443.205 kb on - strand, within Pf1N1B4_4913at 5443.419 kb on + strand, within Pf1N1B4_4913at 5443.552 kb on + strand, within Pf1N1B4_4913

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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5,441,402 - -0.2
5,441,402 - -1.0
5,441,441 - +0.6
5,441,441 - +1.6
5,441,441 - -1.4
5,442,045 + +1.1
5,442,125 - +0.6
5,442,125 - -0.7
5,442,161 - -1.0
5,442,164 - -1.4
5,442,303 + Pf1N1B4_4912 0.31 -1.4
5,442,306 - Pf1N1B4_4912 0.32 -1.1
5,442,316 - -0.4
5,442,362 - Pf1N1B4_4912 0.43 -2.2
5,442,443 - Pf1N1B4_4912 0.60 -0.4
5,442,565 - Pf1N1B4_4912 0.85 -0.6
5,442,963 + +0.2
5,443,008 + -1.1
5,443,009 - +1.2
5,443,009 - +0.6
5,443,009 - +0.6
5,443,205 - Pf1N1B4_4913 0.15 +1.0
5,443,419 + Pf1N1B4_4913 0.43 +0.2
5,443,552 + Pf1N1B4_4913 0.60 -0.0

Or see this region's nucleotide sequence