Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4681 and Pf1N1B4_4682 are separated by 231 nucleotides Pf1N1B4_4682 and Pf1N1B4_4683 are separated by 128 nucleotides Pf1N1B4_4683 and Pf1N1B4_4684 are separated by 21 nucleotides
Pf1N1B4_4681 - LmbE-like protein, at 5,168,020 to 5,168,778
_4681
Pf1N1B4_4682 - Glycogen debranching enzyme (EC 3.2.1.-), at 5,169,010 to 5,171,169
_4682
Pf1N1B4_4683 - FIG00953356: hypothetical protein, at 5,171,298 to 5,171,645
_4683
Pf1N1B4_4684 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 5,171,667 to 5,174,465
_4684
Position (kb) 5169
5170
5171
5172 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5168.022 kb on - strand at 5168.124 kb on - strand, within Pf1N1B4_4681 at 5168.130 kb on - strand, within Pf1N1B4_4681 at 5168.130 kb on - strand, within Pf1N1B4_4681 at 5168.145 kb on + strand, within Pf1N1B4_4681 at 5168.500 kb on + strand at 5168.500 kb on + strand, within Pf1N1B4_4681 at 5168.501 kb on - strand, within Pf1N1B4_4681 at 5168.547 kb on - strand, within Pf1N1B4_4681 at 5168.547 kb on - strand, within Pf1N1B4_4681 at 5168.547 kb on - strand, within Pf1N1B4_4681 at 5168.550 kb on + strand, within Pf1N1B4_4681 at 5168.865 kb on - strand at 5168.869 kb on - strand at 5169.270 kb on + strand, within Pf1N1B4_4682 at 5169.271 kb on - strand, within Pf1N1B4_4682 at 5169.271 kb on - strand, within Pf1N1B4_4682 at 5169.372 kb on - strand at 5169.372 kb on - strand, within Pf1N1B4_4682 at 5169.372 kb on - strand, within Pf1N1B4_4682 at 5169.513 kb on - strand, within Pf1N1B4_4682 at 5169.741 kb on - strand at 5170.214 kb on + strand at 5170.215 kb on - strand, within Pf1N1B4_4682 at 5170.215 kb on - strand at 5170.215 kb on - strand, within Pf1N1B4_4682 at 5170.215 kb on - strand, within Pf1N1B4_4682 at 5170.247 kb on + strand, within Pf1N1B4_4682 at 5170.316 kb on + strand, within Pf1N1B4_4682 at 5170.898 kb on + strand, within Pf1N1B4_4682 at 5170.913 kb on + strand, within Pf1N1B4_4682 at 5170.943 kb on + strand, within Pf1N1B4_4682 at 5170.967 kb on + strand at 5171.169 kb on + strand at 5171.169 kb on + strand at 5171.752 kb on - strand at 5171.752 kb on - strand at 5171.887 kb on - strand at 5171.889 kb on + strand at 5171.889 kb on + strand at 5171.974 kb on + strand, within Pf1N1B4_4684 at 5171.975 kb on - strand at 5171.975 kb on - strand, within Pf1N1B4_4684 at 5171.975 kb on - strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.122 kb on - strand, within Pf1N1B4_4684
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 5,168,022 - +0.6 5,168,124 - Pf1N1B4_4681 0.14 +1.2 5,168,130 - Pf1N1B4_4681 0.14 -0.2 5,168,130 - Pf1N1B4_4681 0.14 +0.0 5,168,145 + Pf1N1B4_4681 0.16 -1.2 5,168,500 + +0.6 5,168,500 + Pf1N1B4_4681 0.63 +0.6 5,168,501 - Pf1N1B4_4681 0.63 +0.6 5,168,547 - Pf1N1B4_4681 0.69 -2.6 5,168,547 - Pf1N1B4_4681 0.69 -0.3 5,168,547 - Pf1N1B4_4681 0.69 +0.6 5,168,550 + Pf1N1B4_4681 0.70 -0.4 5,168,865 - -0.0 5,168,869 - -0.1 5,169,270 + Pf1N1B4_4682 0.12 +1.6 5,169,271 - Pf1N1B4_4682 0.12 +1.4 5,169,271 - Pf1N1B4_4682 0.12 +2.9 5,169,372 - -0.4 5,169,372 - Pf1N1B4_4682 0.17 +1.3 5,169,372 - Pf1N1B4_4682 0.17 +0.2 5,169,513 - Pf1N1B4_4682 0.23 +0.6 5,169,741 - -0.4 5,170,214 + +0.6 5,170,215 - Pf1N1B4_4682 0.56 -0.7 5,170,215 - -0.8 5,170,215 - Pf1N1B4_4682 0.56 +0.1 5,170,215 - Pf1N1B4_4682 0.56 +1.1 5,170,247 + Pf1N1B4_4682 0.57 -0.2 5,170,316 + Pf1N1B4_4682 0.60 +1.8 5,170,898 + Pf1N1B4_4682 0.87 +1.4 5,170,913 + Pf1N1B4_4682 0.88 +2.6 5,170,943 + Pf1N1B4_4682 0.89 +0.6 5,170,967 + +1.6 5,171,169 + +0.5 5,171,169 + +1.3 5,171,752 - -1.0 5,171,752 - -0.4 5,171,887 - +0.2 5,171,889 + +2.9 5,171,889 + -0.2 5,171,974 + Pf1N1B4_4684 0.11 -2.4 5,171,975 - +0.6 5,171,975 - Pf1N1B4_4684 0.11 -0.4 5,171,975 - Pf1N1B4_4684 0.11 +0.2 5,172,093 + Pf1N1B4_4684 0.15 +0.0 5,172,093 + Pf1N1B4_4684 0.15 +1.6 5,172,093 + Pf1N1B4_4684 0.15 -0.0 5,172,093 + Pf1N1B4_4684 0.15 +1.2 5,172,122 - Pf1N1B4_4684 0.16 -0.8
Or see this region's nucleotide sequence