Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4528

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4527 and Pf1N1B4_4528 are separated by 24 nucleotidesPf1N1B4_4528 and Pf1N1B4_4529 are separated by 190 nucleotidesPf1N1B4_4529 and Pf1N1B4_4530 are separated by 336 nucleotides Pf1N1B4_4527 - Short-chain dehydrogenase/reductase SDR, at 4,991,158 to 4,991,925 _4527 Pf1N1B4_4528 - Predicted aminoglycoside phosphotransferase, at 4,991,950 to 4,993,017 _4528 Pf1N1B4_4529 - FIG00966904: hypothetical protein, at 4,993,208 to 4,993,603 _4529 Pf1N1B4_4530 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 4,993,940 to 4,994,482 _4530 Position (kb) 4991 4992 4993 4994Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4990.963 kb on + strandat 4990.964 kb on - strandat 4990.978 kb on - strandat 4990.978 kb on - strandat 4991.105 kb on - strandat 4991.105 kb on - strandat 4991.204 kb on + strandat 4991.205 kb on - strandat 4991.228 kb on + strandat 4991.228 kb on + strandat 4991.228 kb on + strandat 4991.228 kb on + strandat 4991.229 kb on - strandat 4991.318 kb on - strand, within Pf1N1B4_4527at 4991.444 kb on + strand, within Pf1N1B4_4527at 4991.444 kb on + strand, within Pf1N1B4_4527at 4991.444 kb on + strand, within Pf1N1B4_4527at 4991.445 kb on - strandat 4991.445 kb on - strandat 4991.445 kb on - strandat 4991.445 kb on - strand, within Pf1N1B4_4527at 4991.472 kb on + strand, within Pf1N1B4_4527at 4991.472 kb on + strandat 4991.473 kb on - strand, within Pf1N1B4_4527at 4992.062 kb on + strand, within Pf1N1B4_4528at 4992.062 kb on + strand, within Pf1N1B4_4528at 4992.063 kb on - strand, within Pf1N1B4_4528at 4992.063 kb on - strand, within Pf1N1B4_4528at 4992.101 kb on + strandat 4992.107 kb on + strand, within Pf1N1B4_4528at 4992.108 kb on - strand, within Pf1N1B4_4528at 4992.110 kb on + strand, within Pf1N1B4_4528at 4992.111 kb on - strandat 4992.152 kb on + strand, within Pf1N1B4_4528at 4992.447 kb on + strandat 4992.512 kb on + strand, within Pf1N1B4_4528at 4992.555 kb on - strand, within Pf1N1B4_4528at 4992.686 kb on + strand, within Pf1N1B4_4528at 4992.728 kb on + strand, within Pf1N1B4_4528at 4992.728 kb on + strand, within Pf1N1B4_4528at 4992.729 kb on - strand, within Pf1N1B4_4528at 4992.729 kb on - strand, within Pf1N1B4_4528at 4993.008 kb on + strandat 4993.030 kb on + strandat 4993.065 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.107 kb on - strandat 4993.107 kb on - strandat 4993.107 kb on - strandat 4993.167 kb on - strandat 4993.273 kb on - strand, within Pf1N1B4_4529at 4993.739 kb on + strandat 4993.868 kb on - strandat 4993.868 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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4,990,963 + +0.0
4,990,964 - +0.6
4,990,978 - -0.6
4,990,978 - +0.3
4,991,105 - -1.7
4,991,105 - +1.6
4,991,204 + -0.5
4,991,205 - -0.7
4,991,228 + +1.1
4,991,228 + -0.9
4,991,228 + -0.9
4,991,228 + +0.8
4,991,229 - +0.3
4,991,318 - Pf1N1B4_4527 0.21 +0.6
4,991,444 + Pf1N1B4_4527 0.37 -1.7
4,991,444 + Pf1N1B4_4527 0.37 +0.4
4,991,444 + Pf1N1B4_4527 0.37 +1.5
4,991,445 - +0.6
4,991,445 - +1.6
4,991,445 - -1.7
4,991,445 - Pf1N1B4_4527 0.37 +0.1
4,991,472 + Pf1N1B4_4527 0.41 +0.6
4,991,472 + -0.4
4,991,473 - Pf1N1B4_4527 0.41 +0.4
4,992,062 + Pf1N1B4_4528 0.10 -0.1
4,992,062 + Pf1N1B4_4528 0.10 +0.9
4,992,063 - Pf1N1B4_4528 0.11 +1.1
4,992,063 - Pf1N1B4_4528 0.11 +0.9
4,992,101 + +0.6
4,992,107 + Pf1N1B4_4528 0.15 +0.6
4,992,108 - Pf1N1B4_4528 0.15 -1.7
4,992,110 + Pf1N1B4_4528 0.15 +0.6
4,992,111 - +1.6
4,992,152 + Pf1N1B4_4528 0.19 -0.1
4,992,447 + +3.2
4,992,512 + Pf1N1B4_4528 0.53 -1.7
4,992,555 - Pf1N1B4_4528 0.57 -0.4
4,992,686 + Pf1N1B4_4528 0.69 +1.6
4,992,728 + Pf1N1B4_4528 0.73 -0.4
4,992,728 + Pf1N1B4_4528 0.73 -1.4
4,992,729 - Pf1N1B4_4528 0.73 +0.8
4,992,729 - Pf1N1B4_4528 0.73 +1.2
4,993,008 + -0.4
4,993,030 + -1.2
4,993,065 + -0.4
4,993,106 + -0.4
4,993,106 + -0.9
4,993,106 + -1.4
4,993,106 + +1.1
4,993,106 + -0.1
4,993,107 - -1.9
4,993,107 - +1.1
4,993,107 - +1.1
4,993,167 - -0.1
4,993,273 - Pf1N1B4_4529 0.16 -1.5
4,993,739 + -0.8
4,993,868 - +1.1
4,993,868 - -0.9

Or see this region's nucleotide sequence