Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4513 and Pf1N1B4_4514 are separated by 252 nucleotides Pf1N1B4_4514 and Pf1N1B4_4515 are separated by 112 nucleotides Pf1N1B4_4515 and Pf1N1B4_4516 are separated by 0 nucleotides
Pf1N1B4_4513 - hypothetical protein, at 4,975,700 to 4,975,846
_4513
Pf1N1B4_4514 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 4,976,099 to 4,976,719
_4514
Pf1N1B4_4515 - Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2), at 4,976,832 to 4,978,106
_4515
Pf1N1B4_4516 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 4,978,107 to 4,979,474
_4516
Position (kb)
4976
4977
4978
4979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4976.020 kb on + strand at 4976.246 kb on + strand, within Pf1N1B4_4514 at 4976.249 kb on + strand, within Pf1N1B4_4514 at 4976.249 kb on + strand, within Pf1N1B4_4514 at 4976.250 kb on - strand, within Pf1N1B4_4514 at 4976.250 kb on - strand, within Pf1N1B4_4514 at 4976.274 kb on - strand, within Pf1N1B4_4514 at 4976.274 kb on - strand, within Pf1N1B4_4514 at 4976.565 kb on + strand at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.568 kb on - strand at 4976.568 kb on - strand, within Pf1N1B4_4514 at 4976.568 kb on - strand, within Pf1N1B4_4514 at 4976.620 kb on + strand, within Pf1N1B4_4514 at 4976.621 kb on - strand, within Pf1N1B4_4514 at 4976.984 kb on - strand at 4976.984 kb on - strand, within Pf1N1B4_4515 at 4976.984 kb on - strand, within Pf1N1B4_4515 at 4976.988 kb on - strand, within Pf1N1B4_4515 at 4976.988 kb on - strand, within Pf1N1B4_4515 at 4977.036 kb on - strand, within Pf1N1B4_4515 at 4977.106 kb on + strand, within Pf1N1B4_4515 at 4977.107 kb on - strand, within Pf1N1B4_4515 at 4977.122 kb on - strand at 4977.262 kb on - strand, within Pf1N1B4_4515 at 4977.607 kb on + strand at 4977.607 kb on + strand, within Pf1N1B4_4515 at 4977.608 kb on - strand at 4977.608 kb on - strand, within Pf1N1B4_4515 at 4977.641 kb on - strand, within Pf1N1B4_4515 at 4977.898 kb on - strand, within Pf1N1B4_4515 at 4978.022 kb on - strand at 4978.108 kb on + strand at 4978.109 kb on - strand at 4978.109 kb on - strand at 4978.225 kb on + strand at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.513 kb on - strand, within Pf1N1B4_4516 at 4978.702 kb on + strand, within Pf1N1B4_4516 at 4978.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,976,020 + +0.4 4,976,246 + Pf1N1B4_4514 0.24 +2.2 4,976,249 + Pf1N1B4_4514 0.24 +0.9 4,976,249 + Pf1N1B4_4514 0.24 -0.7 4,976,250 - Pf1N1B4_4514 0.24 -1.7 4,976,250 - Pf1N1B4_4514 0.24 -0.4 4,976,274 - Pf1N1B4_4514 0.28 +0.1 4,976,274 - Pf1N1B4_4514 0.28 -0.9 4,976,565 + -0.9 4,976,567 + Pf1N1B4_4514 0.75 -0.5 4,976,567 + Pf1N1B4_4514 0.75 +0.1 4,976,567 + Pf1N1B4_4514 0.75 +0.1 4,976,567 + Pf1N1B4_4514 0.75 -0.2 4,976,567 + Pf1N1B4_4514 0.75 -0.4 4,976,567 + Pf1N1B4_4514 0.75 +0.6 4,976,568 - -0.4 4,976,568 - Pf1N1B4_4514 0.76 +0.3 4,976,568 - Pf1N1B4_4514 0.76 -1.1 4,976,620 + Pf1N1B4_4514 0.84 -0.5 4,976,621 - Pf1N1B4_4514 0.84 -1.2 4,976,984 - +1.6 4,976,984 - Pf1N1B4_4515 0.12 -0.7 4,976,984 - Pf1N1B4_4515 0.12 -0.7 4,976,988 - Pf1N1B4_4515 0.12 -0.4 4,976,988 - Pf1N1B4_4515 0.12 -0.1 4,977,036 - Pf1N1B4_4515 0.16 +0.6 4,977,106 + Pf1N1B4_4515 0.21 -1.1 4,977,107 - Pf1N1B4_4515 0.22 -0.7 4,977,122 - +1.6 4,977,262 - Pf1N1B4_4515 0.34 -1.4 4,977,607 + +0.6 4,977,607 + Pf1N1B4_4515 0.61 -1.4 4,977,608 - +0.6 4,977,608 - Pf1N1B4_4515 0.61 -2.2 4,977,641 - Pf1N1B4_4515 0.63 +0.6 4,977,898 - Pf1N1B4_4515 0.84 -0.4 4,978,022 - +0.8 4,978,108 + -1.7 4,978,109 - +2.2 4,978,109 - -1.4 4,978,225 + -0.5 4,978,512 + Pf1N1B4_4516 0.30 -0.1 4,978,512 + Pf1N1B4_4516 0.30 +0.5 4,978,512 + Pf1N1B4_4516 0.30 +0.3 4,978,512 + Pf1N1B4_4516 0.30 +0.6 4,978,513 - Pf1N1B4_4516 0.30 -0.8 4,978,702 + Pf1N1B4_4516 0.43 -2.8 4,978,703 - -1.4
Or see this region's nucleotide sequence