Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4515

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4513 and Pf1N1B4_4514 are separated by 252 nucleotidesPf1N1B4_4514 and Pf1N1B4_4515 are separated by 112 nucleotidesPf1N1B4_4515 and Pf1N1B4_4516 are separated by 0 nucleotides Pf1N1B4_4513 - hypothetical protein, at 4,975,700 to 4,975,846 _4513 Pf1N1B4_4514 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 4,976,099 to 4,976,719 _4514 Pf1N1B4_4515 - Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2), at 4,976,832 to 4,978,106 _4515 Pf1N1B4_4516 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 4,978,107 to 4,979,474 _4516 Position (kb) 4976 4977 4978 4979Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4976.020 kb on + strandat 4976.246 kb on + strand, within Pf1N1B4_4514at 4976.249 kb on + strand, within Pf1N1B4_4514at 4976.249 kb on + strand, within Pf1N1B4_4514at 4976.250 kb on - strand, within Pf1N1B4_4514at 4976.250 kb on - strand, within Pf1N1B4_4514at 4976.274 kb on - strand, within Pf1N1B4_4514at 4976.274 kb on - strand, within Pf1N1B4_4514at 4976.565 kb on + strandat 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.567 kb on + strand, within Pf1N1B4_4514at 4976.568 kb on - strandat 4976.568 kb on - strand, within Pf1N1B4_4514at 4976.568 kb on - strand, within Pf1N1B4_4514at 4976.620 kb on + strand, within Pf1N1B4_4514at 4976.621 kb on - strand, within Pf1N1B4_4514at 4976.984 kb on - strandat 4976.984 kb on - strand, within Pf1N1B4_4515at 4976.984 kb on - strand, within Pf1N1B4_4515at 4976.988 kb on - strand, within Pf1N1B4_4515at 4976.988 kb on - strand, within Pf1N1B4_4515at 4977.036 kb on - strand, within Pf1N1B4_4515at 4977.106 kb on + strand, within Pf1N1B4_4515at 4977.107 kb on - strand, within Pf1N1B4_4515at 4977.122 kb on - strandat 4977.262 kb on - strand, within Pf1N1B4_4515at 4977.607 kb on + strandat 4977.607 kb on + strand, within Pf1N1B4_4515at 4977.608 kb on - strandat 4977.608 kb on - strand, within Pf1N1B4_4515at 4977.641 kb on - strand, within Pf1N1B4_4515at 4977.898 kb on - strand, within Pf1N1B4_4515at 4978.022 kb on - strandat 4978.108 kb on + strandat 4978.109 kb on - strandat 4978.109 kb on - strandat 4978.225 kb on + strandat 4978.512 kb on + strand, within Pf1N1B4_4516at 4978.512 kb on + strand, within Pf1N1B4_4516at 4978.512 kb on + strand, within Pf1N1B4_4516at 4978.512 kb on + strand, within Pf1N1B4_4516at 4978.513 kb on - strand, within Pf1N1B4_4516at 4978.702 kb on + strand, within Pf1N1B4_4516at 4978.703 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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4,976,020 + +0.4
4,976,246 + Pf1N1B4_4514 0.24 +2.2
4,976,249 + Pf1N1B4_4514 0.24 +0.9
4,976,249 + Pf1N1B4_4514 0.24 -0.7
4,976,250 - Pf1N1B4_4514 0.24 -1.7
4,976,250 - Pf1N1B4_4514 0.24 -0.4
4,976,274 - Pf1N1B4_4514 0.28 +0.1
4,976,274 - Pf1N1B4_4514 0.28 -0.9
4,976,565 + -0.9
4,976,567 + Pf1N1B4_4514 0.75 -0.5
4,976,567 + Pf1N1B4_4514 0.75 +0.1
4,976,567 + Pf1N1B4_4514 0.75 +0.1
4,976,567 + Pf1N1B4_4514 0.75 -0.2
4,976,567 + Pf1N1B4_4514 0.75 -0.4
4,976,567 + Pf1N1B4_4514 0.75 +0.6
4,976,568 - -0.4
4,976,568 - Pf1N1B4_4514 0.76 +0.3
4,976,568 - Pf1N1B4_4514 0.76 -1.1
4,976,620 + Pf1N1B4_4514 0.84 -0.5
4,976,621 - Pf1N1B4_4514 0.84 -1.2
4,976,984 - +1.6
4,976,984 - Pf1N1B4_4515 0.12 -0.7
4,976,984 - Pf1N1B4_4515 0.12 -0.7
4,976,988 - Pf1N1B4_4515 0.12 -0.4
4,976,988 - Pf1N1B4_4515 0.12 -0.1
4,977,036 - Pf1N1B4_4515 0.16 +0.6
4,977,106 + Pf1N1B4_4515 0.21 -1.1
4,977,107 - Pf1N1B4_4515 0.22 -0.7
4,977,122 - +1.6
4,977,262 - Pf1N1B4_4515 0.34 -1.4
4,977,607 + +0.6
4,977,607 + Pf1N1B4_4515 0.61 -1.4
4,977,608 - +0.6
4,977,608 - Pf1N1B4_4515 0.61 -2.2
4,977,641 - Pf1N1B4_4515 0.63 +0.6
4,977,898 - Pf1N1B4_4515 0.84 -0.4
4,978,022 - +0.8
4,978,108 + -1.7
4,978,109 - +2.2
4,978,109 - -1.4
4,978,225 + -0.5
4,978,512 + Pf1N1B4_4516 0.30 -0.1
4,978,512 + Pf1N1B4_4516 0.30 +0.5
4,978,512 + Pf1N1B4_4516 0.30 +0.3
4,978,512 + Pf1N1B4_4516 0.30 +0.6
4,978,513 - Pf1N1B4_4516 0.30 -0.8
4,978,702 + Pf1N1B4_4516 0.43 -2.8
4,978,703 - -1.4

Or see this region's nucleotide sequence