Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4455 and Pf1N1B4_4456 overlap by 4 nucleotides Pf1N1B4_4456 and Pf1N1B4_4457 overlap by 14 nucleotides Pf1N1B4_4457 and Pf1N1B4_4458 overlap by 4 nucleotides
Pf1N1B4_4455 - FIG00953528: hypothetical protein, at 4,915,941 to 4,916,585
_4455
Pf1N1B4_4456 - Short-chain dehydrogenase/reductase SDR, at 4,916,582 to 4,917,391
_4456
Pf1N1B4_4457 - long-chain acyl-CoA synthetase, at 4,917,378 to 4,918,052
_4457
Pf1N1B4_4458 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,918,049 to 4,919,530
_4458
Position (kb)
4917
4918
4919 Strain fitness (log2 ratio)
-2
-1
0
1 at 4916.719 kb on - strand, within Pf1N1B4_4456 at 4916.862 kb on + strand, within Pf1N1B4_4456 at 4916.976 kb on + strand at 4916.976 kb on + strand, within Pf1N1B4_4456 at 4917.185 kb on + strand at 4917.436 kb on + strand at 4917.437 kb on - strand at 4917.446 kb on - strand, within Pf1N1B4_4457 at 4917.562 kb on + strand, within Pf1N1B4_4457 at 4917.563 kb on - strand, within Pf1N1B4_4457 at 4917.812 kb on - strand, within Pf1N1B4_4457 at 4917.826 kb on + strand, within Pf1N1B4_4457 at 4917.826 kb on + strand, within Pf1N1B4_4457 at 4917.827 kb on - strand, within Pf1N1B4_4457 at 4917.919 kb on + strand, within Pf1N1B4_4457 at 4917.920 kb on - strand, within Pf1N1B4_4457 at 4917.920 kb on - strand, within Pf1N1B4_4457 at 4918.429 kb on - strand, within Pf1N1B4_4458 at 4918.662 kb on + strand, within Pf1N1B4_4458 at 4918.662 kb on + strand at 4918.663 kb on - strand, within Pf1N1B4_4458
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,916,719 - Pf1N1B4_4456 0.17 -2.7 4,916,862 + Pf1N1B4_4456 0.35 +0.7 4,916,976 + -1.7 4,916,976 + Pf1N1B4_4456 0.49 -0.3 4,917,185 + +0.1 4,917,436 + +0.7 4,917,437 - -0.6 4,917,446 - Pf1N1B4_4457 0.10 -0.3 4,917,562 + Pf1N1B4_4457 0.27 +1.2 4,917,563 - Pf1N1B4_4457 0.27 -0.3 4,917,812 - Pf1N1B4_4457 0.64 -1.7 4,917,826 + Pf1N1B4_4457 0.66 -0.5 4,917,826 + Pf1N1B4_4457 0.66 +1.7 4,917,827 - Pf1N1B4_4457 0.67 -1.5 4,917,919 + Pf1N1B4_4457 0.80 -1.3 4,917,920 - Pf1N1B4_4457 0.80 -0.8 4,917,920 - Pf1N1B4_4457 0.80 -0.7 4,918,429 - Pf1N1B4_4458 0.26 -0.1 4,918,662 + Pf1N1B4_4458 0.41 +0.6 4,918,662 + +1.4 4,918,663 - Pf1N1B4_4458 0.41 -2.2
Or see this region's nucleotide sequence